Giant fluctuations and structural effects in a flocking epithelium

Fabio Giavazzi, Chiara Malinverno, Salvatore Corallino, Francesco Ginelli, Giorgio Scita, Roberto Cerbino

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Abstract

Epithelial cells cultured in a monolayer are very motile in isolation but reach a near-jammed state when mitotic division increases their number above a critical threshold. We have recently shown that a monolayer can be reawakened by over-expression of a single protein, RAB5A, a master regulator of endocytosis. This reawakening of motility was explained in term of a flocking transition that promotes the emergence of a large-scale collective migratory pattern. Here we focus on the impact of this reawakening on the structural properties of the monolayer. We find that the unjammed monolayer is characterised by a fluidisation at the single cell level and by enhanced non-equilibrium large-scale number fluctuations at a larger length scale. Also with the help of numerical simulations, we trace back the origin of these fluctuations to the 
 self-propelled active nature of the constituents and to the existence of a local alignment mechanism, leading to the spontaneous breaking of the orientational symmetry.
Original languageEnglish
Article number384003
Pages (from-to)1-11
Number of pages11
JournalJournal of Physics D: Applied Physics
Volume50
Early online date13 Jul 2017
DOIs
Publication statusPublished - 30 Aug 2017

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epithelium
Monolayers
locomotion
regulators
cultured cells
division
isolation
Fluidization
alignment
proteins
Structural properties
thresholds
symmetry
cells
Proteins
Computer simulation
simulation

Keywords

  • active matter
  • jamming transition
  • collective cell migration
  • non-equilibrium fluctuations

Cite this

Giavazzi, F., Malinverno, C., Corallino, S., Ginelli, F., Scita, G., & Cerbino, R. (2017). Giant fluctuations and structural effects in a flocking epithelium. Journal of Physics D: Applied Physics, 50, 1-11. [384003]. https://doi.org/10.1088/1361-6463/aa7f8e

Giant fluctuations and structural effects in a flocking epithelium. / Giavazzi, Fabio; Malinverno, Chiara; Corallino, Salvatore; Ginelli, Francesco; Scita, Giorgio; Cerbino, Roberto.

In: Journal of Physics D: Applied Physics, Vol. 50, 384003, 30.08.2017, p. 1-11.

Research output: Contribution to journalArticle

Giavazzi, F, Malinverno, C, Corallino, S, Ginelli, F, Scita, G & Cerbino, R 2017, 'Giant fluctuations and structural effects in a flocking epithelium', Journal of Physics D: Applied Physics, vol. 50, 384003, pp. 1-11. https://doi.org/10.1088/1361-6463/aa7f8e
Giavazzi F, Malinverno C, Corallino S, Ginelli F, Scita G, Cerbino R. Giant fluctuations and structural effects in a flocking epithelium. Journal of Physics D: Applied Physics. 2017 Aug 30;50:1-11. 384003. https://doi.org/10.1088/1361-6463/aa7f8e
Giavazzi, Fabio ; Malinverno, Chiara ; Corallino, Salvatore ; Ginelli, Francesco ; Scita, Giorgio ; Cerbino, Roberto. / Giant fluctuations and structural effects in a flocking epithelium. In: Journal of Physics D: Applied Physics. 2017 ; Vol. 50. pp. 1-11.
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abstract = "Epithelial cells cultured in a monolayer are very motile in isolation but reach a near-jammed state when mitotic division increases their number above a critical threshold. We have recently shown that a monolayer can be reawakened by over-expression of a single protein, RAB5A, a master regulator of endocytosis. This reawakening of motility was explained in term of a flocking transition that promotes the emergence of a large-scale collective migratory pattern. Here we focus on the impact of this reawakening on the structural properties of the monolayer. We find that the unjammed monolayer is characterised by a fluidisation at the single cell level and by enhanced non-equilibrium large-scale number fluctuations at a larger length scale. Also with the help of numerical simulations, we trace back the origin of these fluctuations to the self-propelled active nature of the constituents and to the existence of a local alignment mechanism, leading to the spontaneous breaking of the orientational symmetry.",
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note = "We thank S Henkes for useful discussions. FGia and RC acknowledge funding from the Italian Ministry of University and Scientific Research (MIUR) under the program Futuro in Ricerca—Project ANISOFT (RBFR125H0M) and from Regione Lombardia and CARIPLO foundation under the joint action Avviso congiunto per l'incremento dell'attrattivit{\'a} del sistema ricerca lombardo e della competitivit{\'a} dei ricercatori candidati su strumenti ERC—Project 2016-0998. CM, SC and GS acknowledge funding from Associazione Italiana per la Ricerca sul Cancro (AIRC 10168 and 18621), MIUR, the Italian Ministry of Health, Ricerca Finalizzata (RF0235844), Worldwide Cancer Research (AICR-14-0335), and the European Research Council (Advanced-ERC-268836). CM was also supported by Fondazione Umberto Veronesi and SC by an AIRC fellowship. FGin acknowledges support from the Marie Curie Career Integration Grant (CIG) PCIG13-GA-2013-618399, and wish to thank the University of Milan and LibrOsteria for their hospitality while this work was underway.",
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N1 - We thank S Henkes for useful discussions. FGia and RC acknowledge funding from the Italian Ministry of University and Scientific Research (MIUR) under the program Futuro in Ricerca—Project ANISOFT (RBFR125H0M) and from Regione Lombardia and CARIPLO foundation under the joint action Avviso congiunto per l'incremento dell'attrattivitá del sistema ricerca lombardo e della competitivitá dei ricercatori candidati su strumenti ERC—Project 2016-0998. CM, SC and GS acknowledge funding from Associazione Italiana per la Ricerca sul Cancro (AIRC 10168 and 18621), MIUR, the Italian Ministry of Health, Ricerca Finalizzata (RF0235844), Worldwide Cancer Research (AICR-14-0335), and the European Research Council (Advanced-ERC-268836). CM was also supported by Fondazione Umberto Veronesi and SC by an AIRC fellowship. FGin acknowledges support from the Marie Curie Career Integration Grant (CIG) PCIG13-GA-2013-618399, and wish to thank the University of Milan and LibrOsteria for their hospitality while this work was underway.

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