Global changes in the hippocampal proteome following exposure to an enriched environment

K. McNair, J. Broad, G. Riedel, C. H. Davies, S. R. Cobb

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Abstract

Exposure to an enriched environment promotes neurochemical, structural and neurophysiological changes in the brain and is associated with enhanced synaptic plasticity and improved hippocampal-dependent learning. Using a global proteomics-based approach we have now been able to reveal the altered expression of a diverse range of hippocampal proteins following exposure to an enriched environment. Male Hooded Lister rats (8 weeks) were subjected to a 6-week regimen in which they were housed in either non-enriched (open field) or enriched conditions (toys, wheels etc.). Whole protein extracts from stratum pyramidale and stratum radiatum of area CA1 were then isolated and subjected to differential gel electrophoresis [McNair K, Davies CH, Cobb SR (2006) Plasticity-related regulation of the hippocampal proteome. Eur J Neurosci 23(2):575-580]. Of the 2469 resolvable protein spots detected in this study, 42 spots (1.7% of the detectable proteome) derived from predominantly somatic fractions and 32 proteins spots from dendritic fractions (1.3% of detectable proteome) were significantly altered in abundance following exposure to an enriched environment (somatic: 14 increased/28 decreased abundance, range -1.5 to +1.4-fold change; dendritic: 16 increased, 16 decreased abundance, range -1.6 to +3.0-fold change). Following in-gel tryptic digestion and Maidi-Tof/Q-star mass spectrometry, database searching revealed the identity of 50 protein spots displaying environmental enrichment-related modulation of expression. Identified proteins belonged to a variety of functional classes with gene ontology analysis revealing the majority (> 70%) of regulated proteins to be part of the energy metabolism, cytoplasmic organization/biogenesis and signal transduction processes. (c) 2006 IBRO. Published by Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)413-422
Number of pages10
JournalNeuroscience
Volume145
Issue number2
Early online date29 Jan 2007
DOIs
Publication statusPublished - 16 Mar 2007

Keywords

  • hippocampus
  • environmental enrichment
  • proteomics
  • glutamate
  • plasticity
  • proteome
  • long-term potentiation
  • difference gel-electrophoresis
  • rat hippocampus
  • in-vitro
  • memory
  • expression
  • kinase
  • mice
  • CA1
  • immunoreactivity

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