Global changes in the hippocampal proteome following exposure to an enriched environment

K. McNair, J. Broad, G. Riedel, C. H. Davies, S. R. Cobb

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Exposure to an enriched environment promotes neurochemical, structural and neurophysiological changes in the brain and is associated with enhanced synaptic plasticity and improved hippocampal-dependent learning. Using a global proteomics-based approach we have now been able to reveal the altered expression of a diverse range of hippocampal proteins following exposure to an enriched environment. Male Hooded Lister rats (8 weeks) were subjected to a 6-week regimen in which they were housed in either non-enriched (open field) or enriched conditions (toys, wheels etc.). Whole protein extracts from stratum pyramidale and stratum radiatum of area CA1 were then isolated and subjected to differential gel electrophoresis [McNair K, Davies CH, Cobb SR (2006) Plasticity-related regulation of the hippocampal proteome. Eur J Neurosci 23(2):575-580]. Of the 2469 resolvable protein spots detected in this study, 42 spots (1.7% of the detectable proteome) derived from predominantly somatic fractions and 32 proteins spots from dendritic fractions (1.3% of detectable proteome) were significantly altered in abundance following exposure to an enriched environment (somatic: 14 increased/28 decreased abundance, range -1.5 to +1.4-fold change; dendritic: 16 increased, 16 decreased abundance, range -1.6 to +3.0-fold change). Following in-gel tryptic digestion and Maidi-Tof/Q-star mass spectrometry, database searching revealed the identity of 50 protein spots displaying environmental enrichment-related modulation of expression. Identified proteins belonged to a variety of functional classes with gene ontology analysis revealing the majority (> 70%) of regulated proteins to be part of the energy metabolism, cytoplasmic organization/biogenesis and signal transduction processes. (c) 2006 IBRO. Published by Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)413-422
Number of pages10
JournalNeuroscience
Volume145
Issue number2
Early online date29 Jan 2007
DOIs
Publication statusPublished - 16 Mar 2007

Keywords

  • hippocampus
  • environmental enrichment
  • proteomics
  • glutamate
  • plasticity
  • proteome
  • long-term potentiation
  • difference gel-electrophoresis
  • rat hippocampus
  • in-vitro
  • memory
  • expression
  • kinase
  • mice
  • CA1
  • immunoreactivity

Cite this

Global changes in the hippocampal proteome following exposure to an enriched environment. / McNair, K.; Broad, J.; Riedel, G.; Davies, C. H.; Cobb, S. R.

In: Neuroscience, Vol. 145, No. 2, 16.03.2007, p. 413-422.

Research output: Contribution to journalArticle

McNair, K. ; Broad, J. ; Riedel, G. ; Davies, C. H. ; Cobb, S. R. / Global changes in the hippocampal proteome following exposure to an enriched environment. In: Neuroscience. 2007 ; Vol. 145, No. 2. pp. 413-422.
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AB - Exposure to an enriched environment promotes neurochemical, structural and neurophysiological changes in the brain and is associated with enhanced synaptic plasticity and improved hippocampal-dependent learning. Using a global proteomics-based approach we have now been able to reveal the altered expression of a diverse range of hippocampal proteins following exposure to an enriched environment. Male Hooded Lister rats (8 weeks) were subjected to a 6-week regimen in which they were housed in either non-enriched (open field) or enriched conditions (toys, wheels etc.). Whole protein extracts from stratum pyramidale and stratum radiatum of area CA1 were then isolated and subjected to differential gel electrophoresis [McNair K, Davies CH, Cobb SR (2006) Plasticity-related regulation of the hippocampal proteome. Eur J Neurosci 23(2):575-580]. Of the 2469 resolvable protein spots detected in this study, 42 spots (1.7% of the detectable proteome) derived from predominantly somatic fractions and 32 proteins spots from dendritic fractions (1.3% of detectable proteome) were significantly altered in abundance following exposure to an enriched environment (somatic: 14 increased/28 decreased abundance, range -1.5 to +1.4-fold change; dendritic: 16 increased, 16 decreased abundance, range -1.6 to +3.0-fold change). Following in-gel tryptic digestion and Maidi-Tof/Q-star mass spectrometry, database searching revealed the identity of 50 protein spots displaying environmental enrichment-related modulation of expression. Identified proteins belonged to a variety of functional classes with gene ontology analysis revealing the majority (> 70%) of regulated proteins to be part of the energy metabolism, cytoplasmic organization/biogenesis and signal transduction processes. (c) 2006 IBRO. Published by Elsevier Ltd. All rights reserved.

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