GLUCANOHYDROLASE ENZYME-ACTIVITY IN EMBRYOS OF SCOTS, CORSICAN AND LODGEPOLE PINES INFECTED IN-VITRO WITH HETEROBASIDION-ANNOSUM

V R NSOLOMO, Stephen Woodward

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Embryos of Scots pine (Pinus sylvestris), Corsican pine (P. nigra var. maritima) and Lodgepole pine (P. contorta) were infected in vitro with Heterobasidion annosum and the activities of 1,3-beta-glucanase, chitinase and beta-glucosidase were monitored. Both the infected and control embryos of the three pine species showed the presence of the enzymes. Statistical analysis showed no significant increase (P > 0.05) in the levels of 1,3-beta-glucanase in infected embryos for all the three pine species. However, there was a significant increase in levels of chitinase in infected embryos of P. contorta and P. nigra var. maritima and also in levels of beta-glucosidase in infected embryos of all three of the pine species. The study has therefore revealed that constitutive levels of glucanohydrolase enzymes exist in the embryos of the three pine species and that the induction of chitinase and beta-glucosidase was a factor in the response of the embryos to stimulation by H. annosum infection.

Original languageEnglish
Pages (from-to)144-153
Number of pages10
JournalEuropean Journal of Forest Pathology
Volume24
Issue number3
Publication statusPublished - Jun 1994

Keywords

  • PINES
  • HETEROBASIDION-ANNOSUM
  • 1,3-BETA-GLUCANASE
  • CHITINASE
  • BETA-GLUCOSIDASE
  • IN-VITRO ACTIVITY
  • VERTICILLIUM-ALBO-ATRUM
  • CHITINASE ACTIVITY
  • CULTURED-CELLS
  • PLANT-CELLS
  • PICEA-ABIES
  • RESISTANCE
  • TOMATO
  • INDUCTION
  • ROOTS
  • LYSIS

Cite this