@misc{751f879719cd4bcab06ba5c3a97804dd,
title = "Glutamate Receptor Probing with Rapid Application and Solution Exchange (RASE)",
abstract = "Probing of glutamate receptors at sub-millisecond time scale is a key element needed for understanding of their response kinetics, neural signal transduction, and, in a wider context, intercellular cross talk. One of the classical techniques used to obtain this type of data in electrophysiology is placing a recording pipette in front of a double-barrel solution application pipette, which provides a rapid switch between two solutions. Here we describe a modification of this classical technique, which utilizes a solution application pipette with several loading capillaries. Such a system is capable of replacing multiple application solutions within 7–12 s time intervals. This modified protocol enables ultrafast application of several solutions at the same set of receptors, thus allowing powerful paired-sample statistical approaches. In addition, the same experimental equipment fabricated for the ultra-resolution probing of receptor kinetics can also be applied in several other types of electrophysiological experiments.",
author = "Nathanael O{\textquoteright}Neill and Sergiy Sylantyev",
year = "2019",
doi = "10.1007/978-1-4939-9077-1_6",
language = "English",
isbn = "978-1-4939-9076-4",
volume = "1941",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
address = "United States",
type = "Other",
}