Glycosylation of Candida albicans cell wall proteins is critical for induction of innate immune responses and apoptosis of epithelial cells

Jeanette Wagener, Günther Weindl, Piet W J de Groot, Albert D de Boer, Susanne Kaesler, Selvam Thavaraj, Oliver Bader, Daniela Mailänder-Sanchez, Claudia Borelli, Michael Weig, Tilo Biedermann, Julian R Naglik, Hans Christian Korting, Martin Schaller

Research output: Contribution to journalArticle

15 Citations (Scopus)
3 Downloads (Pure)

Abstract

C. albicans is one of the most common fungal pathogen of humans, causing local and superficial mucosal infections in immunocompromised individuals. Given that the key structure mediating host-C. albicans interactions is the fungal cell wall, we aimed to identify features of the cell wall inducing epithelial responses and be associated with fungal pathogenesis. We demonstrate here the importance of cell wall protein glycosylation in epithelial immune activation with a predominant role for the highly branched N-glycosylation residues. Moreover, these glycan moieties induce growth arrest and apoptosis of epithelial cells. Using an in vitro model of oral candidosis we demonstrate, that apoptosis induction by C. albicans wild-type occurs in early stage of infection and strongly depends on intact cell wall protein glycosylation. These novel findings demonstrate that glycosylation of the C. albicans cell wall proteins appears essential for modulation of epithelial immunity and apoptosis induction, both of which may promote fungal pathogenesis in vivo.
Original languageEnglish
Article numbere50518
Number of pages13
JournalPloS ONE
Volume7
Issue number11
DOIs
Publication statusPublished - 30 Nov 2012

Fingerprint

Glycosylation
Candida
glycosylation
Candida albicans
Innate Immunity
Cell Wall
epithelial cells
apoptosis
Epithelial Cells
Cells
cell walls
Apoptosis
Proteins
proteins
pathogenesis
immunocompromised population
Oral Candidiasis
candidiasis
Pathogens
Infection

Cite this

Glycosylation of Candida albicans cell wall proteins is critical for induction of innate immune responses and apoptosis of epithelial cells. / Wagener, Jeanette; Weindl, Günther; de Groot, Piet W J; de Boer, Albert D; Kaesler, Susanne; Thavaraj, Selvam; Bader, Oliver; Mailänder-Sanchez, Daniela; Borelli, Claudia; Weig, Michael; Biedermann, Tilo; Naglik, Julian R; Korting, Hans Christian; Schaller, Martin.

In: PloS ONE, Vol. 7, No. 11, e50518, 30.11.2012.

Research output: Contribution to journalArticle

Wagener, J, Weindl, G, de Groot, PWJ, de Boer, AD, Kaesler, S, Thavaraj, S, Bader, O, Mailänder-Sanchez, D, Borelli, C, Weig, M, Biedermann, T, Naglik, JR, Korting, HC & Schaller, M 2012, 'Glycosylation of Candida albicans cell wall proteins is critical for induction of innate immune responses and apoptosis of epithelial cells', PloS ONE, vol. 7, no. 11, e50518. https://doi.org/10.1371/journal.pone.0050518
Wagener, Jeanette ; Weindl, Günther ; de Groot, Piet W J ; de Boer, Albert D ; Kaesler, Susanne ; Thavaraj, Selvam ; Bader, Oliver ; Mailänder-Sanchez, Daniela ; Borelli, Claudia ; Weig, Michael ; Biedermann, Tilo ; Naglik, Julian R ; Korting, Hans Christian ; Schaller, Martin. / Glycosylation of Candida albicans cell wall proteins is critical for induction of innate immune responses and apoptosis of epithelial cells. In: PloS ONE. 2012 ; Vol. 7, No. 11.
@article{ca04a387b51f466488daad5513d2900a,
title = "Glycosylation of Candida albicans cell wall proteins is critical for induction of innate immune responses and apoptosis of epithelial cells",
abstract = "C. albicans is one of the most common fungal pathogen of humans, causing local and superficial mucosal infections in immunocompromised individuals. Given that the key structure mediating host-C. albicans interactions is the fungal cell wall, we aimed to identify features of the cell wall inducing epithelial responses and be associated with fungal pathogenesis. We demonstrate here the importance of cell wall protein glycosylation in epithelial immune activation with a predominant role for the highly branched N-glycosylation residues. Moreover, these glycan moieties induce growth arrest and apoptosis of epithelial cells. Using an in vitro model of oral candidosis we demonstrate, that apoptosis induction by C. albicans wild-type occurs in early stage of infection and strongly depends on intact cell wall protein glycosylation. These novel findings demonstrate that glycosylation of the C. albicans cell wall proteins appears essential for modulation of epithelial immunity and apoptosis induction, both of which may promote fungal pathogenesis in vivo.",
author = "Jeanette Wagener and G{\"u}nther Weindl and {de Groot}, {Piet W J} and {de Boer}, {Albert D} and Susanne Kaesler and Selvam Thavaraj and Oliver Bader and Daniela Mail{\"a}nder-Sanchez and Claudia Borelli and Michael Weig and Tilo Biedermann and Naglik, {Julian R} and Korting, {Hans Christian} and Martin Schaller",
year = "2012",
month = "11",
day = "30",
doi = "10.1371/journal.pone.0050518",
language = "English",
volume = "7",
journal = "PloS ONE",
issn = "1932-6203",
publisher = "PUBLIC LIBRARY SCIENCE",
number = "11",

}

TY - JOUR

T1 - Glycosylation of Candida albicans cell wall proteins is critical for induction of innate immune responses and apoptosis of epithelial cells

AU - Wagener, Jeanette

AU - Weindl, Günther

AU - de Groot, Piet W J

AU - de Boer, Albert D

AU - Kaesler, Susanne

AU - Thavaraj, Selvam

AU - Bader, Oliver

AU - Mailänder-Sanchez, Daniela

AU - Borelli, Claudia

AU - Weig, Michael

AU - Biedermann, Tilo

AU - Naglik, Julian R

AU - Korting, Hans Christian

AU - Schaller, Martin

PY - 2012/11/30

Y1 - 2012/11/30

N2 - C. albicans is one of the most common fungal pathogen of humans, causing local and superficial mucosal infections in immunocompromised individuals. Given that the key structure mediating host-C. albicans interactions is the fungal cell wall, we aimed to identify features of the cell wall inducing epithelial responses and be associated with fungal pathogenesis. We demonstrate here the importance of cell wall protein glycosylation in epithelial immune activation with a predominant role for the highly branched N-glycosylation residues. Moreover, these glycan moieties induce growth arrest and apoptosis of epithelial cells. Using an in vitro model of oral candidosis we demonstrate, that apoptosis induction by C. albicans wild-type occurs in early stage of infection and strongly depends on intact cell wall protein glycosylation. These novel findings demonstrate that glycosylation of the C. albicans cell wall proteins appears essential for modulation of epithelial immunity and apoptosis induction, both of which may promote fungal pathogenesis in vivo.

AB - C. albicans is one of the most common fungal pathogen of humans, causing local and superficial mucosal infections in immunocompromised individuals. Given that the key structure mediating host-C. albicans interactions is the fungal cell wall, we aimed to identify features of the cell wall inducing epithelial responses and be associated with fungal pathogenesis. We demonstrate here the importance of cell wall protein glycosylation in epithelial immune activation with a predominant role for the highly branched N-glycosylation residues. Moreover, these glycan moieties induce growth arrest and apoptosis of epithelial cells. Using an in vitro model of oral candidosis we demonstrate, that apoptosis induction by C. albicans wild-type occurs in early stage of infection and strongly depends on intact cell wall protein glycosylation. These novel findings demonstrate that glycosylation of the C. albicans cell wall proteins appears essential for modulation of epithelial immunity and apoptosis induction, both of which may promote fungal pathogenesis in vivo.

U2 - 10.1371/journal.pone.0050518

DO - 10.1371/journal.pone.0050518

M3 - Article

VL - 7

JO - PloS ONE

JF - PloS ONE

SN - 1932-6203

IS - 11

M1 - e50518

ER -