Golgi-dependent transport of cholesterol to the Chlamydia trachomatis inclusion

Reynaldo A Carabeo, David J Mead, Ted Hackstadt

Research output: Contribution to journalArticle

192 Citations (Scopus)

Abstract

Cholesterol, a lipid not normally found in prokaryotes, was identified in purified Chlamydia trachomatis elementary bodies and in the chlamydial parasitophorous vacuole (inclusion) membrane of infected HeLa cells. Chlamydiae obtained eukaryotic host cell cholesterol both from de novo synthesis or low-density lipoprotein. Acquisition of either de novo-synthesized cholesterol or low-density lipoprotein-derived cholesterol was microtubule-dependent and brefeldin A-sensitive, indicating a requirement for the Golgi apparatus. Transport also required chlamydial protein synthesis, indicative of a pathogen-directed process. The cholesterol trafficking pathway appears to coincide with a previously characterized delivery of sphingomyelin to the inclusion in that similar pharmacological treatments inhibited transport of both sphingomyelin and cholesterol. These results support the hypothesis that sphingomyelin and cholesterol may be cotransported via a Golgi-dependent pathway and that the chlamydial inclusion receives cholesterol preferentially from a brefeldin A-sensitive pathway of cholesterol trafficking from the Golgi apparatus to the plasma membrane.
Original languageEnglish
Pages (from-to)6771-6
Number of pages6
JournalPNAS
Volume100
Issue number11
DOIs
Publication statusPublished - 27 May 2003

Fingerprint

Chlamydia trachomatis
Cholesterol
Sphingomyelins
Brefeldin A
Golgi Apparatus
Chlamydia
Eukaryotic Cells
Vacuoles
HeLa Cells
LDL Lipoproteins
Microtubules
LDL Cholesterol
Cell Membrane
Pharmacology
Lipids
Membranes

Keywords

  • Microscopy, Fluorescence
  • Animals
  • HeLa Cells
  • Humans
  • Biological Transport
  • Chlamydia trachomatis
  • CHO Cells
  • Golgi Apparatus
  • Cholesterol
  • Sphingomyelins
  • Cell Line
  • Cricetinae

Cite this

Golgi-dependent transport of cholesterol to the Chlamydia trachomatis inclusion. / Carabeo, Reynaldo A; Mead, David J; Hackstadt, Ted.

In: PNAS, Vol. 100, No. 11, 27.05.2003, p. 6771-6.

Research output: Contribution to journalArticle

Carabeo, Reynaldo A ; Mead, David J ; Hackstadt, Ted. / Golgi-dependent transport of cholesterol to the Chlamydia trachomatis inclusion. In: PNAS. 2003 ; Vol. 100, No. 11. pp. 6771-6.
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AB - Cholesterol, a lipid not normally found in prokaryotes, was identified in purified Chlamydia trachomatis elementary bodies and in the chlamydial parasitophorous vacuole (inclusion) membrane of infected HeLa cells. Chlamydiae obtained eukaryotic host cell cholesterol both from de novo synthesis or low-density lipoprotein. Acquisition of either de novo-synthesized cholesterol or low-density lipoprotein-derived cholesterol was microtubule-dependent and brefeldin A-sensitive, indicating a requirement for the Golgi apparatus. Transport also required chlamydial protein synthesis, indicative of a pathogen-directed process. The cholesterol trafficking pathway appears to coincide with a previously characterized delivery of sphingomyelin to the inclusion in that similar pharmacological treatments inhibited transport of both sphingomyelin and cholesterol. These results support the hypothesis that sphingomyelin and cholesterol may be cotransported via a Golgi-dependent pathway and that the chlamydial inclusion receives cholesterol preferentially from a brefeldin A-sensitive pathway of cholesterol trafficking from the Golgi apparatus to the plasma membrane.

KW - Microscopy, Fluorescence

KW - Animals

KW - HeLa Cells

KW - Humans

KW - Biological Transport

KW - Chlamydia trachomatis

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KW - Golgi Apparatus

KW - Cholesterol

KW - Sphingomyelins

KW - Cell Line

KW - Cricetinae

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