Gonadotropin surge-attenuating factor bioactivity is present in follicular-fluid from naturally cycling women

Paul Alfred Francois Fowler, U. Fahy, M. D. Culler, P. G. Knight, P. G. Wardle, E. A. McLaughlin, P. Cunningham, M. Fraser, M. G. R. Hull, A. Templeton

Research output: Contribution to journalArticle

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Abstract

Rat pituitary monolayer bioassays were used to compare gonadotrophin surge-attenuating factor (GnSAF) bioactivity in follicular fluid from 12 follicles in 10 spontaneously cycling women with that in pooled follicular fluid from women undergoing ovulation induction. Expressed as ED(50)s (mu l follicular fluid/well producing 50% of maximal effect), GnSAF bioactivity was detectable in all spontaneous follicular fluid samples (1.4-33.3 mu l/well) and in follicular fluid from women undergoing ovulation induction (6.8 mu l/well). This GnSAF bioactivity was unaffected by pre-incubation with an inhibin antibody. When the data were grouped according to whether the recovered oocytes fertilized in vitro or not, the fertilized group contained significantly greater GnSAF bioactivity than the unfertilized group (5.3 +/- 1.1 and 14.1 +/- 2.6 mu l/well respectively, P < 0.05). While both inhibin bioactivity (9.7 +/- 1.4 and 28.9 +/- 12.1 mu l/well) and immunoreactivity (36.8 +/- 2.2 and 21.0 +/- 3.0 and ng/ml) were also greater (P < 0.01) in the fertilized compared with the unfertilized groups respectively, there were no other significant differences between the two groups. We conclude that GnSAF is found in follicular fluid from spontaneously cycling women, supporting in-vivo evidence for the involvement of GnSAF in feedback control of the ovary-pituitary axis.

Original languageEnglish
Pages (from-to)68-74
Number of pages7
JournalHuman Reproduction
Volume10
Issue number1
DOIs
Publication statusPublished - Jan 1995

Keywords

  • FOLLICULAR FLUID
  • GNRH
  • GNSAF
  • SPONTANEOUS CYCLES
  • WOMEN
  • SELF-PRIMING ACTION
  • LUTEINIZING-HORMONE SECRETION
  • FACTOR GNSAF
  • STIMULATING-HORMONE
  • INHIBITING FACTOR
  • INVITRO
  • RATS
  • LHRH
  • IMMUNONEUTRALIZATION
  • MONKEYS

Cite this

Gonadotropin surge-attenuating factor bioactivity is present in follicular-fluid from naturally cycling women. / Fowler, Paul Alfred Francois; Fahy, U.; Culler, M. D.; Knight, P. G.; Wardle, P. G.; McLaughlin, E. A.; Cunningham, P.; Fraser, M.; Hull, M. G. R.; Templeton, A.

In: Human Reproduction, Vol. 10, No. 1, 01.1995, p. 68-74.

Research output: Contribution to journalArticle

Fowler, PAF, Fahy, U, Culler, MD, Knight, PG, Wardle, PG, McLaughlin, EA, Cunningham, P, Fraser, M, Hull, MGR & Templeton, A 1995, 'Gonadotropin surge-attenuating factor bioactivity is present in follicular-fluid from naturally cycling women', Human Reproduction, vol. 10, no. 1, pp. 68-74. https://doi.org/10.1093/humrep/10.1.68
Fowler, Paul Alfred Francois ; Fahy, U. ; Culler, M. D. ; Knight, P. G. ; Wardle, P. G. ; McLaughlin, E. A. ; Cunningham, P. ; Fraser, M. ; Hull, M. G. R. ; Templeton, A. / Gonadotropin surge-attenuating factor bioactivity is present in follicular-fluid from naturally cycling women. In: Human Reproduction. 1995 ; Vol. 10, No. 1. pp. 68-74.
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abstract = "Rat pituitary monolayer bioassays were used to compare gonadotrophin surge-attenuating factor (GnSAF) bioactivity in follicular fluid from 12 follicles in 10 spontaneously cycling women with that in pooled follicular fluid from women undergoing ovulation induction. Expressed as ED(50)s (mu l follicular fluid/well producing 50{\%} of maximal effect), GnSAF bioactivity was detectable in all spontaneous follicular fluid samples (1.4-33.3 mu l/well) and in follicular fluid from women undergoing ovulation induction (6.8 mu l/well). This GnSAF bioactivity was unaffected by pre-incubation with an inhibin antibody. When the data were grouped according to whether the recovered oocytes fertilized in vitro or not, the fertilized group contained significantly greater GnSAF bioactivity than the unfertilized group (5.3 +/- 1.1 and 14.1 +/- 2.6 mu l/well respectively, P < 0.05). While both inhibin bioactivity (9.7 +/- 1.4 and 28.9 +/- 12.1 mu l/well) and immunoreactivity (36.8 +/- 2.2 and 21.0 +/- 3.0 and ng/ml) were also greater (P < 0.01) in the fertilized compared with the unfertilized groups respectively, there were no other significant differences between the two groups. We conclude that GnSAF is found in follicular fluid from spontaneously cycling women, supporting in-vivo evidence for the involvement of GnSAF in feedback control of the ovary-pituitary axis.",
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author = "Fowler, {Paul Alfred Francois} and U. Fahy and Culler, {M. D.} and Knight, {P. G.} and Wardle, {P. G.} and McLaughlin, {E. A.} and P. Cunningham and M. Fraser and Hull, {M. G. R.} and A. Templeton",
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T1 - Gonadotropin surge-attenuating factor bioactivity is present in follicular-fluid from naturally cycling women

AU - Fowler, Paul Alfred Francois

AU - Fahy, U.

AU - Culler, M. D.

AU - Knight, P. G.

AU - Wardle, P. G.

AU - McLaughlin, E. A.

AU - Cunningham, P.

AU - Fraser, M.

AU - Hull, M. G. R.

AU - Templeton, A.

PY - 1995/1

Y1 - 1995/1

N2 - Rat pituitary monolayer bioassays were used to compare gonadotrophin surge-attenuating factor (GnSAF) bioactivity in follicular fluid from 12 follicles in 10 spontaneously cycling women with that in pooled follicular fluid from women undergoing ovulation induction. Expressed as ED(50)s (mu l follicular fluid/well producing 50% of maximal effect), GnSAF bioactivity was detectable in all spontaneous follicular fluid samples (1.4-33.3 mu l/well) and in follicular fluid from women undergoing ovulation induction (6.8 mu l/well). This GnSAF bioactivity was unaffected by pre-incubation with an inhibin antibody. When the data were grouped according to whether the recovered oocytes fertilized in vitro or not, the fertilized group contained significantly greater GnSAF bioactivity than the unfertilized group (5.3 +/- 1.1 and 14.1 +/- 2.6 mu l/well respectively, P < 0.05). While both inhibin bioactivity (9.7 +/- 1.4 and 28.9 +/- 12.1 mu l/well) and immunoreactivity (36.8 +/- 2.2 and 21.0 +/- 3.0 and ng/ml) were also greater (P < 0.01) in the fertilized compared with the unfertilized groups respectively, there were no other significant differences between the two groups. We conclude that GnSAF is found in follicular fluid from spontaneously cycling women, supporting in-vivo evidence for the involvement of GnSAF in feedback control of the ovary-pituitary axis.

AB - Rat pituitary monolayer bioassays were used to compare gonadotrophin surge-attenuating factor (GnSAF) bioactivity in follicular fluid from 12 follicles in 10 spontaneously cycling women with that in pooled follicular fluid from women undergoing ovulation induction. Expressed as ED(50)s (mu l follicular fluid/well producing 50% of maximal effect), GnSAF bioactivity was detectable in all spontaneous follicular fluid samples (1.4-33.3 mu l/well) and in follicular fluid from women undergoing ovulation induction (6.8 mu l/well). This GnSAF bioactivity was unaffected by pre-incubation with an inhibin antibody. When the data were grouped according to whether the recovered oocytes fertilized in vitro or not, the fertilized group contained significantly greater GnSAF bioactivity than the unfertilized group (5.3 +/- 1.1 and 14.1 +/- 2.6 mu l/well respectively, P < 0.05). While both inhibin bioactivity (9.7 +/- 1.4 and 28.9 +/- 12.1 mu l/well) and immunoreactivity (36.8 +/- 2.2 and 21.0 +/- 3.0 and ng/ml) were also greater (P < 0.01) in the fertilized compared with the unfertilized groups respectively, there were no other significant differences between the two groups. We conclude that GnSAF is found in follicular fluid from spontaneously cycling women, supporting in-vivo evidence for the involvement of GnSAF in feedback control of the ovary-pituitary axis.

KW - FOLLICULAR FLUID

KW - GNRH

KW - GNSAF

KW - SPONTANEOUS CYCLES

KW - WOMEN

KW - SELF-PRIMING ACTION

KW - LUTEINIZING-HORMONE SECRETION

KW - FACTOR GNSAF

KW - STIMULATING-HORMONE

KW - INHIBITING FACTOR

KW - INVITRO

KW - RATS

KW - LHRH

KW - IMMUNONEUTRALIZATION

KW - MONKEYS

U2 - 10.1093/humrep/10.1.68

DO - 10.1093/humrep/10.1.68

M3 - Article

VL - 10

SP - 68

EP - 74

JO - Human Reproduction

JF - Human Reproduction

SN - 0268-1161

IS - 1

ER -