Grazers induce strong changes in the enzyme activity, size and composition of microbial functional groups involved in soil N dynamics.

A. K. Patra, L. Abbadie, A. Clays-Josserand, V. Degrange, S. J. Grayston, P. Loiseau, F. Louault, Shahid Mahmood, S. Nazaret, L. Philippot, F. Poly, James Ivor Prosser

Research output: Contribution to journalArticle

Abstract

Enhancement of soil nitrogen (N) cycling by grazing has been observed in many grassland ecosystems. However, whether grazing affects the activity only of the key microbial functional groups driving soil N dynamics or also affects the size (cell number) and/or composition of these groups remains largely unknown. We studied the enzyme activity, size, and composition of five soil microbial communities (total microbial and total bacterial communities, and three functional groups driving N dynamics: nitrifiers, denitrifiers, and free N-2 fixers) in grassland sites experiencing contrasting sheep grazing regimes (one light grazing [LG] site and one intensive grazing [IG] site) at two topographical locations. Enzyme activity was determined by potential carbon mineralization, nitrification, denitrification, and N-2 fixation assays. The size of each community (except N-2 fixers) was measured by the most-probable-number technique. The composition of the total soil microbial community was characterized by phospholipid fatty acid analysis (PLFA), and the genetic structure of the total bacterial community was assessed by ribosomal intergenic spacer analysis. The genetic structures of the ammonia-oxidizing, nitrate-reducing, and N-2- fixing communities were characterized by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) or by polymerase chain reaction and denaturing gradient gel electrophoresis (PCR-DGGE) targeting group-specific genes. Greater enzyme activities, particularly for nitrification, were observed in IG than in LG sites at both topographical locations. The numbers of heterotrophs, nitrifiers, and denitrifiers were higher in IG than in LG sites at both topographical locations. The amplitude of changes in community size was higher than that of community enzyme activity. Phospholipid and nucleic acid analyses showed that the composition/structure of all the communities, except nitrate reducers, differed between IG and LG sites at both locations. For each community, changes in activity were correlated with changes in the occurrence of a few individual PLFAs or DNA fragments. Our results thus indicate that grazing enhances the activity of soil microbial communities but also concurrently induces changes in the size and composition/structure of these communities on the sites studied. Although the generality of our conclusions should be tested in other systems, these results are of major importance for predicting the effects of future disturbances or changed grazing regimes on the functioning of grazed ecosystems.

Original languageEnglish
Pages (from-to)65-80
Number of pages15
JournalEcological Monographs
Volume75
Publication statusPublished - 2005

Keywords

  • carbon mineralization
  • denitrification
  • free N-2 fixation
  • grasslands
  • grazing
  • microbial-functional communities
  • microbial diversity
  • nitrification
  • nitrogen cycle
  • GRADIENT GEL-ELECTROPHORESIS
  • AMMONIA-OXIDIZING BACTERIA
  • 16S RIBOSOMAL-RNA
  • NITRATE-REDUCING COMMUNITY
  • ACETYLENE-REDUCTION ASSAY
  • LEGUME ROOT-NODULES
  • NITROGENASE ACTIVITY
  • CLASS PROTEOBACTERIA
  • MANAGEMENT REGIMENS
  • MOLECULAR ANALYSIS

Cite this

Grazers induce strong changes in the enzyme activity, size and composition of microbial functional groups involved in soil N dynamics. / Patra, A. K.; Abbadie, L.; Clays-Josserand, A.; Degrange, V.; Grayston, S. J.; Loiseau, P.; Louault, F.; Mahmood, Shahid; Nazaret, S.; Philippot, L.; Poly, F.; Prosser, James Ivor.

In: Ecological Monographs, Vol. 75, 2005, p. 65-80.

Research output: Contribution to journalArticle

Patra, AK, Abbadie, L, Clays-Josserand, A, Degrange, V, Grayston, SJ, Loiseau, P, Louault, F, Mahmood, S, Nazaret, S, Philippot, L, Poly, F & Prosser, JI 2005, 'Grazers induce strong changes in the enzyme activity, size and composition of microbial functional groups involved in soil N dynamics.', Ecological Monographs, vol. 75, pp. 65-80.
Patra, A. K. ; Abbadie, L. ; Clays-Josserand, A. ; Degrange, V. ; Grayston, S. J. ; Loiseau, P. ; Louault, F. ; Mahmood, Shahid ; Nazaret, S. ; Philippot, L. ; Poly, F. ; Prosser, James Ivor. / Grazers induce strong changes in the enzyme activity, size and composition of microbial functional groups involved in soil N dynamics. In: Ecological Monographs. 2005 ; Vol. 75. pp. 65-80.
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abstract = "Enhancement of soil nitrogen (N) cycling by grazing has been observed in many grassland ecosystems. However, whether grazing affects the activity only of the key microbial functional groups driving soil N dynamics or also affects the size (cell number) and/or composition of these groups remains largely unknown. We studied the enzyme activity, size, and composition of five soil microbial communities (total microbial and total bacterial communities, and three functional groups driving N dynamics: nitrifiers, denitrifiers, and free N-2 fixers) in grassland sites experiencing contrasting sheep grazing regimes (one light grazing [LG] site and one intensive grazing [IG] site) at two topographical locations. Enzyme activity was determined by potential carbon mineralization, nitrification, denitrification, and N-2 fixation assays. The size of each community (except N-2 fixers) was measured by the most-probable-number technique. The composition of the total soil microbial community was characterized by phospholipid fatty acid analysis (PLFA), and the genetic structure of the total bacterial community was assessed by ribosomal intergenic spacer analysis. The genetic structures of the ammonia-oxidizing, nitrate-reducing, and N-2- fixing communities were characterized by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) or by polymerase chain reaction and denaturing gradient gel electrophoresis (PCR-DGGE) targeting group-specific genes. Greater enzyme activities, particularly for nitrification, were observed in IG than in LG sites at both topographical locations. The numbers of heterotrophs, nitrifiers, and denitrifiers were higher in IG than in LG sites at both topographical locations. The amplitude of changes in community size was higher than that of community enzyme activity. Phospholipid and nucleic acid analyses showed that the composition/structure of all the communities, except nitrate reducers, differed between IG and LG sites at both locations. For each community, changes in activity were correlated with changes in the occurrence of a few individual PLFAs or DNA fragments. Our results thus indicate that grazing enhances the activity of soil microbial communities but also concurrently induces changes in the size and composition/structure of these communities on the sites studied. Although the generality of our conclusions should be tested in other systems, these results are of major importance for predicting the effects of future disturbances or changed grazing regimes on the functioning of grazed ecosystems.",
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TY - JOUR

T1 - Grazers induce strong changes in the enzyme activity, size and composition of microbial functional groups involved in soil N dynamics.

AU - Patra, A. K.

AU - Abbadie, L.

AU - Clays-Josserand, A.

AU - Degrange, V.

AU - Grayston, S. J.

AU - Loiseau, P.

AU - Louault, F.

AU - Mahmood, Shahid

AU - Nazaret, S.

AU - Philippot, L.

AU - Poly, F.

AU - Prosser, James Ivor

PY - 2005

Y1 - 2005

N2 - Enhancement of soil nitrogen (N) cycling by grazing has been observed in many grassland ecosystems. However, whether grazing affects the activity only of the key microbial functional groups driving soil N dynamics or also affects the size (cell number) and/or composition of these groups remains largely unknown. We studied the enzyme activity, size, and composition of five soil microbial communities (total microbial and total bacterial communities, and three functional groups driving N dynamics: nitrifiers, denitrifiers, and free N-2 fixers) in grassland sites experiencing contrasting sheep grazing regimes (one light grazing [LG] site and one intensive grazing [IG] site) at two topographical locations. Enzyme activity was determined by potential carbon mineralization, nitrification, denitrification, and N-2 fixation assays. The size of each community (except N-2 fixers) was measured by the most-probable-number technique. The composition of the total soil microbial community was characterized by phospholipid fatty acid analysis (PLFA), and the genetic structure of the total bacterial community was assessed by ribosomal intergenic spacer analysis. The genetic structures of the ammonia-oxidizing, nitrate-reducing, and N-2- fixing communities were characterized by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) or by polymerase chain reaction and denaturing gradient gel electrophoresis (PCR-DGGE) targeting group-specific genes. Greater enzyme activities, particularly for nitrification, were observed in IG than in LG sites at both topographical locations. The numbers of heterotrophs, nitrifiers, and denitrifiers were higher in IG than in LG sites at both topographical locations. The amplitude of changes in community size was higher than that of community enzyme activity. Phospholipid and nucleic acid analyses showed that the composition/structure of all the communities, except nitrate reducers, differed between IG and LG sites at both locations. For each community, changes in activity were correlated with changes in the occurrence of a few individual PLFAs or DNA fragments. Our results thus indicate that grazing enhances the activity of soil microbial communities but also concurrently induces changes in the size and composition/structure of these communities on the sites studied. Although the generality of our conclusions should be tested in other systems, these results are of major importance for predicting the effects of future disturbances or changed grazing regimes on the functioning of grazed ecosystems.

AB - Enhancement of soil nitrogen (N) cycling by grazing has been observed in many grassland ecosystems. However, whether grazing affects the activity only of the key microbial functional groups driving soil N dynamics or also affects the size (cell number) and/or composition of these groups remains largely unknown. We studied the enzyme activity, size, and composition of five soil microbial communities (total microbial and total bacterial communities, and three functional groups driving N dynamics: nitrifiers, denitrifiers, and free N-2 fixers) in grassland sites experiencing contrasting sheep grazing regimes (one light grazing [LG] site and one intensive grazing [IG] site) at two topographical locations. Enzyme activity was determined by potential carbon mineralization, nitrification, denitrification, and N-2 fixation assays. The size of each community (except N-2 fixers) was measured by the most-probable-number technique. The composition of the total soil microbial community was characterized by phospholipid fatty acid analysis (PLFA), and the genetic structure of the total bacterial community was assessed by ribosomal intergenic spacer analysis. The genetic structures of the ammonia-oxidizing, nitrate-reducing, and N-2- fixing communities were characterized by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) or by polymerase chain reaction and denaturing gradient gel electrophoresis (PCR-DGGE) targeting group-specific genes. Greater enzyme activities, particularly for nitrification, were observed in IG than in LG sites at both topographical locations. The numbers of heterotrophs, nitrifiers, and denitrifiers were higher in IG than in LG sites at both topographical locations. The amplitude of changes in community size was higher than that of community enzyme activity. Phospholipid and nucleic acid analyses showed that the composition/structure of all the communities, except nitrate reducers, differed between IG and LG sites at both locations. For each community, changes in activity were correlated with changes in the occurrence of a few individual PLFAs or DNA fragments. Our results thus indicate that grazing enhances the activity of soil microbial communities but also concurrently induces changes in the size and composition/structure of these communities on the sites studied. Although the generality of our conclusions should be tested in other systems, these results are of major importance for predicting the effects of future disturbances or changed grazing regimes on the functioning of grazed ecosystems.

KW - carbon mineralization

KW - denitrification

KW - free N-2 fixation

KW - grasslands

KW - grazing

KW - microbial-functional communities

KW - microbial diversity

KW - nitrification

KW - nitrogen cycle

KW - GRADIENT GEL-ELECTROPHORESIS

KW - AMMONIA-OXIDIZING BACTERIA

KW - 16S RIBOSOMAL-RNA

KW - NITRATE-REDUCING COMMUNITY

KW - ACETYLENE-REDUCTION ASSAY

KW - LEGUME ROOT-NODULES

KW - NITROGENASE ACTIVITY

KW - CLASS PROTEOBACTERIA

KW - MANAGEMENT REGIMENS

KW - MOLECULAR ANALYSIS

M3 - Article

VL - 75

SP - 65

EP - 80

JO - Ecological Monographs

JF - Ecological Monographs

SN - 0012-9615

ER -