Growth and spectral emission of lux recombinant and bioluminescent marine bacteria

G. Thouand, P. Daniel, H. Habib, P. Picart, M. J. Durand, Kenneth Stuart Killham, O. G. G. Knox, M. S. Dubow, M. Rousseau

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    Abstract

    The purpose of the present paper was to study the influence of bacteria harbouring the luciferase-encoding Vibrio harveyi luxAB genes upon the spectral emission during growth in batch-culture conditions. In vivo bioluminescence spectra were compared from several bioluminescent strains, either naturally luminescent (Vibrio fischeri and Vibrio harveyi) or in recombinant strains (two Gram-negative Escherichia coli::luxAB strains and a Gram-positive Bacillus subtilis::luxAB strain). Spectral emission was recorded from 400 nm to 750 nm using a highly sensitive spectrometer initially devoted to Raman scattering. Two peaks were clearly identified, one at 491-500 nm (+/-5 nm) and a second peak at 585-595 (+/-5 nm) with the Raman CCD. The former peak was the only one detected with traditional spectrometers with a photomultiplier detector commonly used for spectral emission measurement, due to their lack of sensitivity and low resolution in the 550-650 nm window. When spectra were compared between all the studied bacteria, no difference was observed between natural or recombinant cells, between Gram-positive and Gram-negative strains, and growth conditions and growth medium were not found to modify the spectrum of light emission. Copyright (C) 2003 John Wiley Sons, Ltd.

    Original languageEnglish
    Pages (from-to)145-155
    Number of pages10
    JournalLuminescence
    Volume18
    DOIs
    Publication statusPublished - 2003

    Keywords

    • bioluminescence
    • recombinant bacteria
    • spectrum
    • ESCHERICHIA-COLI
    • LUCIFERASE GENES
    • VIBRIO-HARVEYI
    • EXPRESSION
    • BIOSENSOR
    • LIGHT
    • CONSTRUCTION
    • CHEMILUMINESCENCE
    • TOXICITY
    • SENSORS

    Cite this

    Thouand, G., Daniel, P., Habib, H., Picart, P., Durand, M. J., Killham, K. S., Knox, O. G. G., Dubow, M. S., & Rousseau, M. (2003). Growth and spectral emission of lux recombinant and bioluminescent marine bacteria. Luminescence, 18, 145-155. https://doi.org/10.1002/bio.716