Gst-4-dependent suppression of neurodegeneration in c. Elegans models of parkinson’s and machado-joseph disease by rapeseed pomace extract supplementation

Franziska Pohl, Andreia Teixeira-Castro, Marta Daniela Costa, Victoria Lindsay, Juliana Fiúza-Fernandes, Marie Goua, Giovanna Bermano, Wendy Russell, Patrícia Maciel*, Paul Kong Thoo Lin

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Genetic mutations and aging-associated oxidative damage underlie the onset and progression of neurodegenerative diseases, like Parkinson’s disease (PD) and Machado-Joseph disease (MJD). Natural products derived from plants have been regarded as important sources of novel bioactive compounds to counteract neurodegeneration. Here, we tested the neuroprotective effect of an ethanolic extract of rapeseed pomace (RSP), a rapeseed (canola) oil production by-product, in C. elegans models of MJD and PD. The extract, containing sinapine and other phenolics, restored motor function of mutant ataxin-3 (ATXN3) animals (MJD) and prevented degeneration of dopaminergic neurons in one toxin-induced and two genetic models of PD. Wholeorganism sensors of antioxidant and xenobiotic response activation revealed the induction of phase II detoxification enzymes, including glutathione S- transferase (GST-4) upon RSP extract supplementation. Furthermore in vivo pharmacogenetic studies confirmed gst-4 is required for the therapeutic effect of RSP extract in the two disease models. The results suggest that GST-4-mediated antioxidant pathways may constitute promising therapeutic co-targets for neurodegenerative diseases and confirm the utility of searching for bioactive compounds in novel sources, including food and agricultural waste/by-products, such as RSP.

Original languageEnglish
Article number1091
JournalFrontiers in Neuroscience
Volume13
DOIs
Publication statusPublished - 17 Oct 2019

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Machado-Joseph Disease
Brassica rapa
Parkinson Disease
Neurodegenerative Diseases
Phase II Metabolic Detoxication
Antioxidants
Waste Products
Dopaminergic Neurons
Genetic Models
Neuroprotective Agents
Therapeutic Uses
Xenobiotics
Glutathione Transferase
Biological Products
Food
Mutation
Enzymes

Keywords

  • Antioxidant
  • C. Elegans
  • Gst-4
  • Machado-Joseph disease
  • Parkinson’s disease
  • Rapeseed (canola) pomace
  • Sod-3
  • Spinocerebellar ataxia 3
  • C. elegans
  • antioxidant
  • OXIDATIVE STRESS
  • SINAPINE
  • Parkinson's disease
  • sod-3
  • ATAXIN-3
  • SYNUCLEIN
  • DOPAMINERGIC-NEURONS
  • CHONDRUS-CRISPUS
  • gst-4
  • PROTECTS
  • IN-VITRO
  • rapeseed (canola) pomace
  • ANTIOXIDANT
  • TRANSFERASE-ACTIVITY

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Gst-4-dependent suppression of neurodegeneration in c. Elegans models of parkinson’s and machado-joseph disease by rapeseed pomace extract supplementation. / Pohl, Franziska; Teixeira-Castro, Andreia; Costa, Marta Daniela; Lindsay, Victoria; Fiúza-Fernandes, Juliana; Goua, Marie; Bermano, Giovanna; Russell, Wendy; Maciel, Patrícia; Lin, Paul Kong Thoo.

In: Frontiers in Neuroscience, Vol. 13, 1091, 17.10.2019.

Research output: Contribution to journalArticle

Pohl, Franziska ; Teixeira-Castro, Andreia ; Costa, Marta Daniela ; Lindsay, Victoria ; Fiúza-Fernandes, Juliana ; Goua, Marie ; Bermano, Giovanna ; Russell, Wendy ; Maciel, Patrícia ; Lin, Paul Kong Thoo. / Gst-4-dependent suppression of neurodegeneration in c. Elegans models of parkinson’s and machado-joseph disease by rapeseed pomace extract supplementation. In: Frontiers in Neuroscience. 2019 ; Vol. 13.
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