Previously, absorption of L-proline across the apical membrane of the intestinal enterocyte has been attributed to transport via the Na(+)-dependent Imino system. However, net (absorptive) transport of proline across intact Caco-2 cell monolayers was enhanced by acidification of the apical environment, under both Na(+)-containing and Na(+)-free conditions. This Na(+)-independent pH-dependent proline flux was associated with H+ flow across the apical membrane as determined by continuous measurement of intracellular pH. H+/proline symport was associated with an inward Isc in voltage-clamped Caco-2 epithelial layers demonstrating the electrogenic nature of this transport process. In conclusion Caco-2 cells possess an apically-localised, Na(+)-independent, electrogenic H+/imino acid transporter which may play an important role in intestinal proline absorption.
|Number of pages||5|
|Publication status||Published - 25 Oct 1993|
- Biological Transport
- Cell Line
- Epithelial Cells
- Hydrogen-Ion Concentration
- Intestinal Mucosa
Thwaites, D. T., McEwan, G. T., Cook, M. J., Hirst, B. H., & Simmons, N. L. (1993). H(+)-coupled (Na(+)-independent) proline transport in human intestinal (Caco-2) epithelial cell monolayers. FEBS Letters, 333(1-2), 78-82.