TY - JOUR
T1 - Haplotype analysis of hemochromatosis gene polymorphisms in chronic hepatitis C virus infection
T2 - A case control study
AU - Gerayli, Sina
AU - Pasdar, Alireza
AU - Shakeri, Mohammad Taghi
AU - Sepahi, Samaneh
AU - Hoseini, Seyed Mousalreza
AU - Ahadi, Mitra
AU - Rostami, Sina
AU - Meshkat, Zahra
N1 - Funding/Support: This study was financially supported by the research council of Mashhad University of Medical Sciences, Mashhad, Iran (Grant No. 901012).
PY - 2016/6
Y1 - 2016/6
N2 - Background: Chronic hepatitis C virus (HCV) infection is frequently associated with elevated serum iron markers. Polymorphisms in the hemochromatosis (HFE) genes are responsible for iron accumulation in most cases of hemochromatosis, and may play a role in HCV infection. Objectives: We aimed to assess the prevalence of HFE gene polymorphisms in a group of Iranian HCV-infected patients, and to explore the association of these polymorphisms with HCV infection. Patients and Methods: HFE gene polymorphisms were examined in a total of 69 HCV patients and 69 healthy controls using polymerase chain reaction and restriction fragment length polymorphism techniques. Haplotype and diplotype analyses were performed using PHASE software. Results: In a recessive analysismodelof the His63Asp (H63D) locus(HHvs. HD+DD), theHHgenotypewasmorecommonin patients compared to controls (adjusted P = 0.012; OR = 6.42 [95% CI: 1.51 - 27.33]). Also, in a recessive analysis model of the Cys282Tyr (C282Y) locus (CC vs. CY + YY), the CC genotype was more frequent in patients compared to controls (adjusted P = 0.03; OR = 5.06 [95% CI: 1.13 - 22.06]). In addition, there was a significant association between the HC haplotype and the HCDC diplotype and HCV infection. Conclusions: Polymorphism in the hemochromatosis gene may confer some degree of risk for HCV infection, and individuals carrying the H and C alleles may be susceptible to this disease; however, a larger sample of HCV patients and healthy individuals may be necessary to further illustrate the role of these polymorphisms in HCV.
AB - Background: Chronic hepatitis C virus (HCV) infection is frequently associated with elevated serum iron markers. Polymorphisms in the hemochromatosis (HFE) genes are responsible for iron accumulation in most cases of hemochromatosis, and may play a role in HCV infection. Objectives: We aimed to assess the prevalence of HFE gene polymorphisms in a group of Iranian HCV-infected patients, and to explore the association of these polymorphisms with HCV infection. Patients and Methods: HFE gene polymorphisms were examined in a total of 69 HCV patients and 69 healthy controls using polymerase chain reaction and restriction fragment length polymorphism techniques. Haplotype and diplotype analyses were performed using PHASE software. Results: In a recessive analysismodelof the His63Asp (H63D) locus(HHvs. HD+DD), theHHgenotypewasmorecommonin patients compared to controls (adjusted P = 0.012; OR = 6.42 [95% CI: 1.51 - 27.33]). Also, in a recessive analysis model of the Cys282Tyr (C282Y) locus (CC vs. CY + YY), the CC genotype was more frequent in patients compared to controls (adjusted P = 0.03; OR = 5.06 [95% CI: 1.13 - 22.06]). In addition, there was a significant association between the HC haplotype and the HCDC diplotype and HCV infection. Conclusions: Polymorphism in the hemochromatosis gene may confer some degree of risk for HCV infection, and individuals carrying the H and C alleles may be susceptible to this disease; however, a larger sample of HCV patients and healthy individuals may be necessary to further illustrate the role of these polymorphisms in HCV.
KW - Hemochromatosis (HFE) Gene Polymorphisms
KW - Hepatitis C Virus
KW - Prognosis
UR - http://www.scopus.com/inward/record.url?scp=84978967767&partnerID=8YFLogxK
U2 - 10.5812/ircmj.24675
DO - 10.5812/ircmj.24675
M3 - Article
AN - SCOPUS:84978967767
VL - 18
JO - Iranian red crescent medical journal
JF - Iranian red crescent medical journal
SN - 2074-1804
IS - 6
M1 - e24675
ER -