Heat and lime-treatment as effective control methods for E. coli O157:H7 in organic wastes

Lisa M Avery, A Prysor Williams, Ken Killham, David L Jones

    Research output: Contribution to journalArticle

    20 Citations (Scopus)

    Abstract

    Land-application of abattoir wastes is economically appealing and may provide an effective means of closing the nutrient cycling loop. This practise is constrained, however, by legislation which necessitates pre-treatment to remove pathogenic micro-organisms prior to land-spreading. Here we investigated whether heat-treatment or lime addition could eliminate Escherichia coli O157:H7 from three contrasting abattoir wastes. We found that treatment at 60 °C for 10 min effectively eradicated the organism while treatment for the same length of time at 50 °C led to 2-4 log reductions, but not a complete kill. Temperatures of 72 °C induced waste solidification rendering its use impractical. The potential for re-growth in heat-treated and untreated wastes was also investigated. Survival was significantly greater in heat-treated wastes, although the difference was less than half a log unit in magnitude. This effect of heat-treatment on pathogen survival appeared to be ameliorated when wastes were mixed with soil. No viable E. coli O157:H7 cells were recovered from any waste after application of lime (CaO) at a rate of 10 g l , even after enrichment. Our results indicate that pasteurisation-style or liming treatments may provide a suitable alternative method for reducing pathogen loads in abattoir wastes, so that they can be applied to land with minimal biological risk.
    Original languageEnglish
    Pages (from-to)2692-2698
    Number of pages7
    JournalBioresource Technology
    Volume100
    Issue number10
    DOIs
    Publication statusPublished - May 2009

    Fingerprint

    Lime
    Escherichia coli
    lime
    Pathogens
    pathogen
    Heat treatment
    Pasteurization
    Waste heat
    liming
    regrowth
    solidification
    nutrient cycling
    Hot Temperature
    organic waste
    method
    Nutrients
    Solidification
    legislation
    Soils
    land

    Cite this

    Heat and lime-treatment as effective control methods for E. coli O157:H7 in organic wastes. / Avery, Lisa M; Williams, A Prysor; Killham, Ken; Jones, David L.

    In: Bioresource Technology, Vol. 100, No. 10, 05.2009, p. 2692-2698.

    Research output: Contribution to journalArticle

    Avery, Lisa M ; Williams, A Prysor ; Killham, Ken ; Jones, David L. / Heat and lime-treatment as effective control methods for E. coli O157:H7 in organic wastes. In: Bioresource Technology. 2009 ; Vol. 100, No. 10. pp. 2692-2698.
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