Hepatic metabolism of 2-hydroxy-4-methylthiobutyrate in growing lambs

T J Wester, M Vázquez-Añón, J Dibner, D S Parker, Alexander Graham Calder, Gerald Lobley

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

This study was undertaken to determine how, and where, 2-hydroxy-4-methylthiobutyrate (HMTBA) can augment Met metabolism in lambs. Four lambs (initial body weight of 50 kg, SE = 2, and 6 mo of age) prepared with catheters in the mesenteric, portal, hepatic, and jugular veins plus the aorta, were fed at 1.5x maintenance on a grass hay, barley, fish meal, molasses/pre-mix (5:3:1:1, as fed) diet, supplied as hourly meals. Lambs were infused for 10 h with [methyl-2H3]Met (0.11 mmol/h) in a jugular vein and p-aminohippurate into the mesenteric vein. From 1 h onwards, successive 3-h infusions of saline (control), 0.55 mg/min (3.67 micromol/min), and 4.44 mg/min (29.6 micromol/min) of HMTBA were also infused into the mesenteric vein. Plasma, sampled continuously, was collected every 20 min during the last 60 min of each infusion. All infused HMTBA was recovered at the portal vein with 25% extracted subsequently by the liver. Portal appearance of total Cys and Met was unaltered by HMTBA infusion, but net splanchnic appearance of Cys increased (0.04, 0.08, 0.23 mmol/h, SEM = 0.05), whereas Met decreased (0.14, -0.01, -0.21 mmol/h, SED = 0.05). Despite this, arterial Met increased (27.0, 30.7, 51.5 microM, SEM = 2.1) as did Met irreversible loss rate (27.6, 28.7, 40.1 micromol/h, SEM = 0.51), equivalent to 40% of the HMTBA reentering the plasma after conversion to Met. These data indicate that, in ruminants, HMTBA is probably converted to Met within peripheral tissues; that is, where the metabolic need for Met exists.
Original languageEnglish
Pages (from-to)1062-1071
Number of pages10
JournalJournal of Dairy Science
Volume89
Issue number3
DOIs
Publication statusPublished - 1 Mar 2006

Fingerprint

Mesenteric Veins
lambs
Jugular Veins
jugular vein
Portal Vein
liver
metabolism
Meals
Liver
p-Aminohippuric Acid
Molasses
portal vein
Hepatic Veins
Viscera
grass hay
Ruminants
molasses
Hordeum
aorta
Poaceae

Keywords

  • Animals
  • Carbon Isotopes
  • Cysteine
  • Hepatic Veins
  • Isotope Labeling
  • Jugular Veins
  • Liver
  • Mesenteric Veins
  • Methionine
  • Portal Vein
  • Sensitivity and Specificity
  • Sheep
  • p-Aminohippuric Acid

Cite this

Wester, T. J., Vázquez-Añón, M., Dibner, J., Parker, D. S., Calder, A. G., & Lobley, G. (2006). Hepatic metabolism of 2-hydroxy-4-methylthiobutyrate in growing lambs. Journal of Dairy Science, 89(3), 1062-1071. https://doi.org/10.3168/jds.S0022-0302(06)72174-9

Hepatic metabolism of 2-hydroxy-4-methylthiobutyrate in growing lambs. / Wester, T J; Vázquez-Añón, M; Dibner, J; Parker, D S; Calder, Alexander Graham; Lobley, Gerald.

In: Journal of Dairy Science, Vol. 89, No. 3, 01.03.2006, p. 1062-1071.

Research output: Contribution to journalArticle

Wester, TJ, Vázquez-Añón, M, Dibner, J, Parker, DS, Calder, AG & Lobley, G 2006, 'Hepatic metabolism of 2-hydroxy-4-methylthiobutyrate in growing lambs', Journal of Dairy Science, vol. 89, no. 3, pp. 1062-1071. https://doi.org/10.3168/jds.S0022-0302(06)72174-9
Wester, T J ; Vázquez-Añón, M ; Dibner, J ; Parker, D S ; Calder, Alexander Graham ; Lobley, Gerald. / Hepatic metabolism of 2-hydroxy-4-methylthiobutyrate in growing lambs. In: Journal of Dairy Science. 2006 ; Vol. 89, No. 3. pp. 1062-1071.
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abstract = "This study was undertaken to determine how, and where, 2-hydroxy-4-methylthiobutyrate (HMTBA) can augment Met metabolism in lambs. Four lambs (initial body weight of 50 kg, SE = 2, and 6 mo of age) prepared with catheters in the mesenteric, portal, hepatic, and jugular veins plus the aorta, were fed at 1.5x maintenance on a grass hay, barley, fish meal, molasses/pre-mix (5:3:1:1, as fed) diet, supplied as hourly meals. Lambs were infused for 10 h with [methyl-2H3]Met (0.11 mmol/h) in a jugular vein and p-aminohippurate into the mesenteric vein. From 1 h onwards, successive 3-h infusions of saline (control), 0.55 mg/min (3.67 micromol/min), and 4.44 mg/min (29.6 micromol/min) of HMTBA were also infused into the mesenteric vein. Plasma, sampled continuously, was collected every 20 min during the last 60 min of each infusion. All infused HMTBA was recovered at the portal vein with 25{\%} extracted subsequently by the liver. Portal appearance of total Cys and Met was unaltered by HMTBA infusion, but net splanchnic appearance of Cys increased (0.04, 0.08, 0.23 mmol/h, SEM = 0.05), whereas Met decreased (0.14, -0.01, -0.21 mmol/h, SED = 0.05). Despite this, arterial Met increased (27.0, 30.7, 51.5 microM, SEM = 2.1) as did Met irreversible loss rate (27.6, 28.7, 40.1 micromol/h, SEM = 0.51), equivalent to 40{\%} of the HMTBA reentering the plasma after conversion to Met. These data indicate that, in ruminants, HMTBA is probably converted to Met within peripheral tissues; that is, where the metabolic need for Met exists.",
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AU - Wester, T J

AU - Vázquez-Añón, M

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AU - Parker, D S

AU - Calder, Alexander Graham

AU - Lobley, Gerald

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N2 - This study was undertaken to determine how, and where, 2-hydroxy-4-methylthiobutyrate (HMTBA) can augment Met metabolism in lambs. Four lambs (initial body weight of 50 kg, SE = 2, and 6 mo of age) prepared with catheters in the mesenteric, portal, hepatic, and jugular veins plus the aorta, were fed at 1.5x maintenance on a grass hay, barley, fish meal, molasses/pre-mix (5:3:1:1, as fed) diet, supplied as hourly meals. Lambs were infused for 10 h with [methyl-2H3]Met (0.11 mmol/h) in a jugular vein and p-aminohippurate into the mesenteric vein. From 1 h onwards, successive 3-h infusions of saline (control), 0.55 mg/min (3.67 micromol/min), and 4.44 mg/min (29.6 micromol/min) of HMTBA were also infused into the mesenteric vein. Plasma, sampled continuously, was collected every 20 min during the last 60 min of each infusion. All infused HMTBA was recovered at the portal vein with 25% extracted subsequently by the liver. Portal appearance of total Cys and Met was unaltered by HMTBA infusion, but net splanchnic appearance of Cys increased (0.04, 0.08, 0.23 mmol/h, SEM = 0.05), whereas Met decreased (0.14, -0.01, -0.21 mmol/h, SED = 0.05). Despite this, arterial Met increased (27.0, 30.7, 51.5 microM, SEM = 2.1) as did Met irreversible loss rate (27.6, 28.7, 40.1 micromol/h, SEM = 0.51), equivalent to 40% of the HMTBA reentering the plasma after conversion to Met. These data indicate that, in ruminants, HMTBA is probably converted to Met within peripheral tissues; that is, where the metabolic need for Met exists.

AB - This study was undertaken to determine how, and where, 2-hydroxy-4-methylthiobutyrate (HMTBA) can augment Met metabolism in lambs. Four lambs (initial body weight of 50 kg, SE = 2, and 6 mo of age) prepared with catheters in the mesenteric, portal, hepatic, and jugular veins plus the aorta, were fed at 1.5x maintenance on a grass hay, barley, fish meal, molasses/pre-mix (5:3:1:1, as fed) diet, supplied as hourly meals. Lambs were infused for 10 h with [methyl-2H3]Met (0.11 mmol/h) in a jugular vein and p-aminohippurate into the mesenteric vein. From 1 h onwards, successive 3-h infusions of saline (control), 0.55 mg/min (3.67 micromol/min), and 4.44 mg/min (29.6 micromol/min) of HMTBA were also infused into the mesenteric vein. Plasma, sampled continuously, was collected every 20 min during the last 60 min of each infusion. All infused HMTBA was recovered at the portal vein with 25% extracted subsequently by the liver. Portal appearance of total Cys and Met was unaltered by HMTBA infusion, but net splanchnic appearance of Cys increased (0.04, 0.08, 0.23 mmol/h, SEM = 0.05), whereas Met decreased (0.14, -0.01, -0.21 mmol/h, SED = 0.05). Despite this, arterial Met increased (27.0, 30.7, 51.5 microM, SEM = 2.1) as did Met irreversible loss rate (27.6, 28.7, 40.1 micromol/h, SEM = 0.51), equivalent to 40% of the HMTBA reentering the plasma after conversion to Met. These data indicate that, in ruminants, HMTBA is probably converted to Met within peripheral tissues; that is, where the metabolic need for Met exists.

KW - Animals

KW - Carbon Isotopes

KW - Cysteine

KW - Hepatic Veins

KW - Isotope Labeling

KW - Jugular Veins

KW - Liver

KW - Mesenteric Veins

KW - Methionine

KW - Portal Vein

KW - Sensitivity and Specificity

KW - Sheep

KW - p-Aminohippuric Acid

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DO - 10.3168/jds.S0022-0302(06)72174-9

M3 - Article

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EP - 1071

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JF - Journal of Dairy Science

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