Heterobasidion annosum 5.8s ribosomal DNA and internal transcribed spacer sequence: rapid identification of European intersterility groups by ribosomal DNA restriction polymorphism

T Kasuga, C Woods, S Woodward, K Mitchelson

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Using conserved fungal ribosomal gene sequences the internal transcribed spacer (ITS) regions one and two (ITSI, ITS2) and the 5.8s ribosomal RNA gene (rRNA) of Heterobasidion annosum were amplified by the polymerase chain reaction (PCR). The nucleotide sequence was determined in three European intersterility groups (ISG-S, -F and -P). Three sequence variants of the ITS were found in ISG-S isolates. The sequence of the ITS of ISG-F differed by two residues from the major ISG-S sequence variant. The ISG-P sequence differed from ISG-S and ISG-F at 15-16 and 16 residues, respectively. Amplified intergenic spacer elements were informative for ISG fingerprinting following digestion with various 4-cutter restriction endonucleases. All differences in the restriction fragments between the ISGs were because of sequence differences in the ITS regions. The fingerprint patterns of isolates from the same intersterility group but from different European localities were identical. These results show that ribosomal DNA finger-printing is a rapid technique to identify ISGs in Heterobasidion annosum.

Original languageEnglish
Pages (from-to)433-436
Number of pages4
JournalCurrent Genetics
Volume24
Issue number5
DOIs
Publication statusPublished - Nov 1993

Keywords

  • heterobasidion-annosum
  • 5.8S ribosomal-RNA
  • internal transcribed spacer
  • RFLP polymorphism
  • intersterility groups
  • RNA genes

Cite this

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title = "Heterobasidion annosum 5.8s ribosomal DNA and internal transcribed spacer sequence: rapid identification of European intersterility groups by ribosomal DNA restriction polymorphism",
abstract = "Using conserved fungal ribosomal gene sequences the internal transcribed spacer (ITS) regions one and two (ITSI, ITS2) and the 5.8s ribosomal RNA gene (rRNA) of Heterobasidion annosum were amplified by the polymerase chain reaction (PCR). The nucleotide sequence was determined in three European intersterility groups (ISG-S, -F and -P). Three sequence variants of the ITS were found in ISG-S isolates. The sequence of the ITS of ISG-F differed by two residues from the major ISG-S sequence variant. The ISG-P sequence differed from ISG-S and ISG-F at 15-16 and 16 residues, respectively. Amplified intergenic spacer elements were informative for ISG fingerprinting following digestion with various 4-cutter restriction endonucleases. All differences in the restriction fragments between the ISGs were because of sequence differences in the ITS regions. The fingerprint patterns of isolates from the same intersterility group but from different European localities were identical. These results show that ribosomal DNA finger-printing is a rapid technique to identify ISGs in Heterobasidion annosum.",
keywords = "heterobasidion-annosum, 5.8S ribosomal-RNA, internal transcribed spacer, RFLP polymorphism, intersterility groups, RNA genes",
author = "T Kasuga and C Woods and S Woodward and K Mitchelson",
year = "1993",
month = "11",
doi = "10.1007/BF00351853",
language = "English",
volume = "24",
pages = "433--436",
journal = "Current Genetics",
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TY - JOUR

T1 - Heterobasidion annosum 5.8s ribosomal DNA and internal transcribed spacer sequence: rapid identification of European intersterility groups by ribosomal DNA restriction polymorphism

AU - Kasuga, T

AU - Woods, C

AU - Woodward, S

AU - Mitchelson, K

PY - 1993/11

Y1 - 1993/11

N2 - Using conserved fungal ribosomal gene sequences the internal transcribed spacer (ITS) regions one and two (ITSI, ITS2) and the 5.8s ribosomal RNA gene (rRNA) of Heterobasidion annosum were amplified by the polymerase chain reaction (PCR). The nucleotide sequence was determined in three European intersterility groups (ISG-S, -F and -P). Three sequence variants of the ITS were found in ISG-S isolates. The sequence of the ITS of ISG-F differed by two residues from the major ISG-S sequence variant. The ISG-P sequence differed from ISG-S and ISG-F at 15-16 and 16 residues, respectively. Amplified intergenic spacer elements were informative for ISG fingerprinting following digestion with various 4-cutter restriction endonucleases. All differences in the restriction fragments between the ISGs were because of sequence differences in the ITS regions. The fingerprint patterns of isolates from the same intersterility group but from different European localities were identical. These results show that ribosomal DNA finger-printing is a rapid technique to identify ISGs in Heterobasidion annosum.

AB - Using conserved fungal ribosomal gene sequences the internal transcribed spacer (ITS) regions one and two (ITSI, ITS2) and the 5.8s ribosomal RNA gene (rRNA) of Heterobasidion annosum were amplified by the polymerase chain reaction (PCR). The nucleotide sequence was determined in three European intersterility groups (ISG-S, -F and -P). Three sequence variants of the ITS were found in ISG-S isolates. The sequence of the ITS of ISG-F differed by two residues from the major ISG-S sequence variant. The ISG-P sequence differed from ISG-S and ISG-F at 15-16 and 16 residues, respectively. Amplified intergenic spacer elements were informative for ISG fingerprinting following digestion with various 4-cutter restriction endonucleases. All differences in the restriction fragments between the ISGs were because of sequence differences in the ITS regions. The fingerprint patterns of isolates from the same intersterility group but from different European localities were identical. These results show that ribosomal DNA finger-printing is a rapid technique to identify ISGs in Heterobasidion annosum.

KW - heterobasidion-annosum

KW - 5.8S ribosomal-RNA

KW - internal transcribed spacer

KW - RFLP polymorphism

KW - intersterility groups

KW - RNA genes

U2 - 10.1007/BF00351853

DO - 10.1007/BF00351853

M3 - Article

VL - 24

SP - 433

EP - 436

JO - Current Genetics

JF - Current Genetics

SN - 0172-8083

IS - 5

ER -