Hgc1 mediates dynamic Candida albicans-endothelium adhesion events during circulation

Duncan Wilson, Bernhard Hube

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Common iatrogenic procedures can result in translocation of the human pathogenic fungus Candida albicans from mucosal surfaces to the bloodstream. Subsequent disseminated candidiasis and infection of deep-seated organs may occur if the fungus is not eliminated by blood cells. In these cases, fungal cells adhere to the endothelial cells of blood vessels, penetrate through endothelial layers, and invade deeper tissue. In this scenario, endothelial adhesion events must occur during circulation under conditions of physiological blood pressure. To investigate the fungal and host factors which contribute to this essential step of disseminated candidiasis, we have developed an in vitro circulatory C. albicans-endothelium interaction model. We demonstrate that both C. albicans yeast and hyphae can adhere under flow at a pressure similar to capillary blood pressure. Serum factors significantly enhanced the adhesion potential of viable but not killed C. albicans cells to endothelial cells. During circulation, C. albicans cells produced hyphae and the adhesion potential first increased, then decreased with time. We provide evidence that a specific temporal event in the yeast-to-hyphal transition, regulated by the G(1) cyclin Hgc1, is critical for C. albicans-endothelium adhesion during circulation.
Original languageEnglish
Pages (from-to)278-287
Number of pages10
JournalEukaryotic Cell
Volume9
Issue number2
Early online date18 Dec 2009
DOIs
Publication statusPublished - Feb 2010

Fingerprint

Candida albicans
Endothelium
Hyphae
Candidiasis
Cyclin G
Fungi
Endothelial Cells
Yeasts
Blood Pressure
Blood Vessels
Blood Cells
Pressure
Infection
Serum

Keywords

  • Candida albicans
  • cell adhesion
  • cyclin G1
  • endothelial cells
  • endothelium, vascular
  • fungal proteins
  • humans

Cite this

Hgc1 mediates dynamic Candida albicans-endothelium adhesion events during circulation. / Wilson, Duncan; Hube, Bernhard.

In: Eukaryotic Cell, Vol. 9, No. 2, 02.2010, p. 278-287.

Research output: Contribution to journalArticle

Wilson, Duncan ; Hube, Bernhard. / Hgc1 mediates dynamic Candida albicans-endothelium adhesion events during circulation. In: Eukaryotic Cell. 2010 ; Vol. 9, No. 2. pp. 278-287.
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