High-energy phosphate metabolism during exercise and recovery in temperate and Antarctic scallops: An in vivo 31-P-NMR study

David Mark Bailey, L. S. Peck, C. Bock, H. O. Portner

    Research output: Contribution to journalArticle

    40 Citations (Scopus)

    Abstract

    In vivo P-31-nuclear magnetic resonance (NMR) spectroscopy was used to measure the levels of ATP, phospho-L-arginine (PLA), and inorganic phosphate in the adductor muscle of the Antarctic scallop Adamussium colbecki and two temperate species, Aequipecten opercularis and Pecten maximus. Graded exercise regimes from light (one to two contractions) to exhausting (failing to respond to further stimulation) were imposed on animals of each species at its habitat temperature (0degrees vs. 12degreesC, respectively). NMR spectroscopy allowed noninvasive measurement of metabolite levels and intracellular pH at high time resolution (30-120-s intervals) during exercise and throughout the recovery period. Significant differences were shown between the magnitude and form of the metabolic response with increasing levels of exercise in each species. After exhaustion, short-term (first 15 min) muscle alkalosis was followed by acidosis of up to 0.2 pH units during the recovery process. Aequipecten opercularis had similar resting muscle PLA levels compared with either P. maximus or A. colbecki but used a fivefold greater proportion of this store per contraction and was able to perform only half as many claps (maximum of 24) as the other species before exhaustion. All species regenerated their PLA store at a similar rate despite different environmental temperatures. These findings argue for some cold compensation of muscular performance and recovery capacities in the Ant-arctic scallop, albeit at levels of performance similar to scallops with low activity lifestyles from temperate latitudes.

    Original languageEnglish
    Pages (from-to)622-633
    Number of pages11
    JournalPhysiological and Biochemical Zoology
    Volume76
    DOIs
    Publication statusPublished - 2003

    Keywords

    • JUVENILE SEA SCALLOPS
    • PLACOPECTEN-MAGELLANICUS (GMELIN)
    • ASTERIAS-VULGARIS VERRILL
    • VALVE CLOSURE RESPONSES
    • CHLAMYS-OPERCULARIS L
    • HUMAN SKELETAL-MUSCLE
    • CANCER-IRRORATUS SAY
    • ESCAPE RESPONSES
    • ADDUCTOR MUSCLE
    • PECTEN-MAXIMUS

    Cite this

    High-energy phosphate metabolism during exercise and recovery in temperate and Antarctic scallops: An in vivo 31-P-NMR study. / Bailey, David Mark; Peck, L. S.; Bock, C.; Portner, H. O.

    In: Physiological and Biochemical Zoology, Vol. 76, 2003, p. 622-633.

    Research output: Contribution to journalArticle

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    T1 - High-energy phosphate metabolism during exercise and recovery in temperate and Antarctic scallops: An in vivo 31-P-NMR study

    AU - Bailey, David Mark

    AU - Peck, L. S.

    AU - Bock, C.

    AU - Portner, H. O.

    PY - 2003

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    N2 - In vivo P-31-nuclear magnetic resonance (NMR) spectroscopy was used to measure the levels of ATP, phospho-L-arginine (PLA), and inorganic phosphate in the adductor muscle of the Antarctic scallop Adamussium colbecki and two temperate species, Aequipecten opercularis and Pecten maximus. Graded exercise regimes from light (one to two contractions) to exhausting (failing to respond to further stimulation) were imposed on animals of each species at its habitat temperature (0degrees vs. 12degreesC, respectively). NMR spectroscopy allowed noninvasive measurement of metabolite levels and intracellular pH at high time resolution (30-120-s intervals) during exercise and throughout the recovery period. Significant differences were shown between the magnitude and form of the metabolic response with increasing levels of exercise in each species. After exhaustion, short-term (first 15 min) muscle alkalosis was followed by acidosis of up to 0.2 pH units during the recovery process. Aequipecten opercularis had similar resting muscle PLA levels compared with either P. maximus or A. colbecki but used a fivefold greater proportion of this store per contraction and was able to perform only half as many claps (maximum of 24) as the other species before exhaustion. All species regenerated their PLA store at a similar rate despite different environmental temperatures. These findings argue for some cold compensation of muscular performance and recovery capacities in the Ant-arctic scallop, albeit at levels of performance similar to scallops with low activity lifestyles from temperate latitudes.

    AB - In vivo P-31-nuclear magnetic resonance (NMR) spectroscopy was used to measure the levels of ATP, phospho-L-arginine (PLA), and inorganic phosphate in the adductor muscle of the Antarctic scallop Adamussium colbecki and two temperate species, Aequipecten opercularis and Pecten maximus. Graded exercise regimes from light (one to two contractions) to exhausting (failing to respond to further stimulation) were imposed on animals of each species at its habitat temperature (0degrees vs. 12degreesC, respectively). NMR spectroscopy allowed noninvasive measurement of metabolite levels and intracellular pH at high time resolution (30-120-s intervals) during exercise and throughout the recovery period. Significant differences were shown between the magnitude and form of the metabolic response with increasing levels of exercise in each species. After exhaustion, short-term (first 15 min) muscle alkalosis was followed by acidosis of up to 0.2 pH units during the recovery process. Aequipecten opercularis had similar resting muscle PLA levels compared with either P. maximus or A. colbecki but used a fivefold greater proportion of this store per contraction and was able to perform only half as many claps (maximum of 24) as the other species before exhaustion. All species regenerated their PLA store at a similar rate despite different environmental temperatures. These findings argue for some cold compensation of muscular performance and recovery capacities in the Ant-arctic scallop, albeit at levels of performance similar to scallops with low activity lifestyles from temperate latitudes.

    KW - JUVENILE SEA SCALLOPS

    KW - PLACOPECTEN-MAGELLANICUS (GMELIN)

    KW - ASTERIAS-VULGARIS VERRILL

    KW - VALVE CLOSURE RESPONSES

    KW - CHLAMYS-OPERCULARIS L

    KW - HUMAN SKELETAL-MUSCLE

    KW - CANCER-IRRORATUS SAY

    KW - ESCAPE RESPONSES

    KW - ADDUCTOR MUSCLE

    KW - PECTEN-MAXIMUS

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    DO - 10.1086/376920

    M3 - Article

    VL - 76

    SP - 622

    EP - 633

    JO - Physiological and Biochemical Zoology

    JF - Physiological and Biochemical Zoology

    SN - 1522-2152

    ER -