High omega-3:omega-6 fatty acid ratios in culture medium reduce endometrial-cell survival in combined endometrial gland and stromal cell cultures from women with and without endometriosis

M. R. Gazvani, Lorna Margaret Smith, Paul Haggarty, Paul Alfred Francois Fowler, Alexander Allan Templeton

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)

Abstract

Objective: To study the effects of omega -3 and omega -6 polyunsaturated fatty acid (PUFA) on in vitro proliferation of endometrial cells and their production of the cytokine interleukin-8 (IL-8).

Design: In vitro study.

Setting: Obstetrics and gynecology department, University of Aberdeen.

Patient(s): Women attending an infertility clinic.

Intervention(s): In vitro cell cultures using culture mediums supplemented with normal and high ratios of omega -3 PUFA and omega -6 PUFA.

Main Outcome Measure(s): In vitro survival and production of IL-8 by dispersed endometrial cells.

Result(s): In vitro survival of endometrial cells from women with and without endometriosis was significantly reduced in the presence of high omega -3:omega -6 PUFA ratios compared with cells incubated in the absence of fatty acids, in balanced w-3:w-6 PUFA ratios, and in high omega -6:omega -3 PUFA ratios. Endometrial cells from women with endometriosis secreted higher concentrations of IL-8, especially in the presence of high omega -3:omega -6 PUFA ratios.

Conclusion(s): omega -3 PUFA may have a suppressive effect on the in vitro survival of endometrial cells and omega -3 PUFA be useful in the management of endometriosis by reducing the inflammatory response and modulating cytokine function. (Fertil Steril(R) 2001,;76:717-22. (C) 2001 by American Society for Reproductive Medicine.).

Original languageEnglish
Pages (from-to)717-722
Number of pages5
JournalFertility and Sterility
Volume76
Issue number4
DOIs
Publication statusPublished - Oct 2001

Keywords

  • fatty acids
  • endometriosis
  • cytokines
  • prostaglandins
  • cell culture
  • tumor-necrosis-factor
  • peritoneal-fluid
  • arachidonic-acid
  • growth-factor
  • dietary N-3
  • interleukin-8
  • nutrition
  • mediators
  • adhesion

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