High omega-3:omega-6 fatty acid ratios in culture medium reduce endometrial-cell survival in combined endometrial gland and stromal cell cultures from women with and without endometriosis

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Abstract

Objective: To study the effects of omega -3 and omega -6 polyunsaturated fatty acid (PUFA) on in vitro proliferation of endometrial cells and their production of the cytokine interleukin-8 (IL-8).

Design: In vitro study.

Setting: Obstetrics and gynecology department, University of Aberdeen.

Patient(s): Women attending an infertility clinic.

Intervention(s): In vitro cell cultures using culture mediums supplemented with normal and high ratios of omega -3 PUFA and omega -6 PUFA.

Main Outcome Measure(s): In vitro survival and production of IL-8 by dispersed endometrial cells.

Result(s): In vitro survival of endometrial cells from women with and without endometriosis was significantly reduced in the presence of high omega -3:omega -6 PUFA ratios compared with cells incubated in the absence of fatty acids, in balanced w-3:w-6 PUFA ratios, and in high omega -6:omega -3 PUFA ratios. Endometrial cells from women with endometriosis secreted higher concentrations of IL-8, especially in the presence of high omega -3:omega -6 PUFA ratios.

Conclusion(s): omega -3 PUFA may have a suppressive effect on the in vitro survival of endometrial cells and omega -3 PUFA be useful in the management of endometriosis by reducing the inflammatory response and modulating cytokine function. (Fertil Steril(R) 2001,;76:717-22. (C) 2001 by American Society for Reproductive Medicine.).

Original languageEnglish
Pages (from-to)717-722
Number of pages5
JournalFertility and Sterility
Volume76
Issue number4
DOIs
Publication statusPublished - Oct 2001

Keywords

  • fatty acids
  • endometriosis
  • cytokines
  • prostaglandins
  • cell culture
  • tumor-necrosis-factor
  • peritoneal-fluid
  • arachidonic-acid
  • growth-factor
  • dietary N-3
  • interleukin-8
  • nutrition
  • mediators
  • adhesion

Cite this

@article{3af5168473dd46a18864e8bc23dde8b3,
title = "High omega-3:omega-6 fatty acid ratios in culture medium reduce endometrial-cell survival in combined endometrial gland and stromal cell cultures from women with and without endometriosis",
abstract = "Objective: To study the effects of omega -3 and omega -6 polyunsaturated fatty acid (PUFA) on in vitro proliferation of endometrial cells and their production of the cytokine interleukin-8 (IL-8).Design: In vitro study.Setting: Obstetrics and gynecology department, University of Aberdeen.Patient(s): Women attending an infertility clinic.Intervention(s): In vitro cell cultures using culture mediums supplemented with normal and high ratios of omega -3 PUFA and omega -6 PUFA.Main Outcome Measure(s): In vitro survival and production of IL-8 by dispersed endometrial cells.Result(s): In vitro survival of endometrial cells from women with and without endometriosis was significantly reduced in the presence of high omega -3:omega -6 PUFA ratios compared with cells incubated in the absence of fatty acids, in balanced w-3:w-6 PUFA ratios, and in high omega -6:omega -3 PUFA ratios. Endometrial cells from women with endometriosis secreted higher concentrations of IL-8, especially in the presence of high omega -3:omega -6 PUFA ratios.Conclusion(s): omega -3 PUFA may have a suppressive effect on the in vitro survival of endometrial cells and omega -3 PUFA be useful in the management of endometriosis by reducing the inflammatory response and modulating cytokine function. (Fertil Steril(R) 2001,;76:717-22. (C) 2001 by American Society for Reproductive Medicine.).",
keywords = "fatty acids, endometriosis, cytokines, prostaglandins, cell culture, tumor-necrosis-factor, peritoneal-fluid, arachidonic-acid, growth-factor, dietary N-3, interleukin-8, nutrition, mediators, adhesion",
author = "Gazvani, {M. R.} and Smith, {Lorna Margaret} and Paul Haggarty and Fowler, {Paul Alfred Francois} and Templeton, {Alexander Allan}",
year = "2001",
month = "10",
doi = "10.1016/S0015-0282(01)01991-4",
language = "English",
volume = "76",
pages = "717--722",
journal = "Fertility and Sterility",
issn = "0015-0282",
publisher = "ACADEMIC PRESS INC ELSEVIER SCIENCE",
number = "4",

}

TY - JOUR

T1 - High omega-3:omega-6 fatty acid ratios in culture medium reduce endometrial-cell survival in combined endometrial gland and stromal cell cultures from women with and without endometriosis

AU - Gazvani, M. R.

AU - Smith, Lorna Margaret

AU - Haggarty, Paul

AU - Fowler, Paul Alfred Francois

AU - Templeton, Alexander Allan

PY - 2001/10

Y1 - 2001/10

N2 - Objective: To study the effects of omega -3 and omega -6 polyunsaturated fatty acid (PUFA) on in vitro proliferation of endometrial cells and their production of the cytokine interleukin-8 (IL-8).Design: In vitro study.Setting: Obstetrics and gynecology department, University of Aberdeen.Patient(s): Women attending an infertility clinic.Intervention(s): In vitro cell cultures using culture mediums supplemented with normal and high ratios of omega -3 PUFA and omega -6 PUFA.Main Outcome Measure(s): In vitro survival and production of IL-8 by dispersed endometrial cells.Result(s): In vitro survival of endometrial cells from women with and without endometriosis was significantly reduced in the presence of high omega -3:omega -6 PUFA ratios compared with cells incubated in the absence of fatty acids, in balanced w-3:w-6 PUFA ratios, and in high omega -6:omega -3 PUFA ratios. Endometrial cells from women with endometriosis secreted higher concentrations of IL-8, especially in the presence of high omega -3:omega -6 PUFA ratios.Conclusion(s): omega -3 PUFA may have a suppressive effect on the in vitro survival of endometrial cells and omega -3 PUFA be useful in the management of endometriosis by reducing the inflammatory response and modulating cytokine function. (Fertil Steril(R) 2001,;76:717-22. (C) 2001 by American Society for Reproductive Medicine.).

AB - Objective: To study the effects of omega -3 and omega -6 polyunsaturated fatty acid (PUFA) on in vitro proliferation of endometrial cells and their production of the cytokine interleukin-8 (IL-8).Design: In vitro study.Setting: Obstetrics and gynecology department, University of Aberdeen.Patient(s): Women attending an infertility clinic.Intervention(s): In vitro cell cultures using culture mediums supplemented with normal and high ratios of omega -3 PUFA and omega -6 PUFA.Main Outcome Measure(s): In vitro survival and production of IL-8 by dispersed endometrial cells.Result(s): In vitro survival of endometrial cells from women with and without endometriosis was significantly reduced in the presence of high omega -3:omega -6 PUFA ratios compared with cells incubated in the absence of fatty acids, in balanced w-3:w-6 PUFA ratios, and in high omega -6:omega -3 PUFA ratios. Endometrial cells from women with endometriosis secreted higher concentrations of IL-8, especially in the presence of high omega -3:omega -6 PUFA ratios.Conclusion(s): omega -3 PUFA may have a suppressive effect on the in vitro survival of endometrial cells and omega -3 PUFA be useful in the management of endometriosis by reducing the inflammatory response and modulating cytokine function. (Fertil Steril(R) 2001,;76:717-22. (C) 2001 by American Society for Reproductive Medicine.).

KW - fatty acids

KW - endometriosis

KW - cytokines

KW - prostaglandins

KW - cell culture

KW - tumor-necrosis-factor

KW - peritoneal-fluid

KW - arachidonic-acid

KW - growth-factor

KW - dietary N-3

KW - interleukin-8

KW - nutrition

KW - mediators

KW - adhesion

U2 - 10.1016/S0015-0282(01)01991-4

DO - 10.1016/S0015-0282(01)01991-4

M3 - Article

VL - 76

SP - 717

EP - 722

JO - Fertility and Sterility

JF - Fertility and Sterility

SN - 0015-0282

IS - 4

ER -