Abstract
Transmission electron microscopy (TEM) is the main technique used to study the ultrastructure of biological samples. Chemical fixation was considered the main method for preserving samples for TEM; however, it is a relatively slow method of fixation and can result in morphological alterations. Cryofixation using high-pressure freezing (HPF) overcomes the limitations of chemical fixation by preserving samples instantly. Here, we describe our HPF methods optimized for visualizing Candida auris at the ultrastructural level.
Original language | English |
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Title of host publication | Candida auris |
Subtitle of host publication | Methods and Protocols |
Editors | Alexander Lorenz |
Publisher | HUMANA PRESS INC |
Chapter | 15 |
Pages | 189-201 |
Number of pages | 13 |
Edition | 1st |
ISBN (Electronic) | 978-1-0716-2417-3 |
ISBN (Print) | 978-1-0716-2416-6 |
DOIs | |
Publication status | Published - 9 Jun 2022 |
Publication series
Name | Methods in Molecular Biology |
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Volume | 2517 |
ISSN (Print) | 1064-3745 |
ISSN (Electronic) | 1940-6029 |
Bibliographical note
Funding Information:The authors acknowledge the Microscopy and Histology Core Facility at the University of Aberdeen for expert assistance.
Keywords
- Freeze substitution
- Fungal cell wall
- High-pressure freezing
- Transmission electron microscopy
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Microscopy and Histology
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