@inbook{f902442a834e4e0096b63f6df0a3e8e7,
title = "High-Pressure Freezing and Transmission Electron Microscopy to Visualize the Ultrastructure of the C. auris Cell Wall",
abstract = "Transmission electron microscopy (TEM) is the main technique used to study the ultrastructure of biological samples. Chemical fixation was considered the main method for preserving samples for TEM; however, it is a relatively slow method of fixation and can result in morphological alterations. Cryofixation using high-pressure freezing (HPF) overcomes the limitations of chemical fixation by preserving samples instantly. Here, we describe our HPF methods optimized for visualizing Candida auris at the ultrastructural level.",
keywords = "Freeze substitution, Fungal cell wall, High-pressure freezing, Transmission electron microscopy",
author = "Gillian Milne and Walker, {Louise A.}",
note = "Funding Information: The authors acknowledge the Microscopy and Histology Core Facility at the University of Aberdeen for expert assistance. ",
year = "2022",
doi = "10.1007/978-1-0716-2417-3_15",
language = "English",
series = "Methods in Molecular Biology",
publisher = "HUMANA PRESS INC",
pages = "189--201",
booktitle = "Methods in Molecular Biology",
}