High-resolution synchrotron imaging shows that root hairs influence rhizosphere soil structure formation

N. Koebernik, K. R. Daly, S. D. Keyes, T. S. George, L .K. Brown, A. Raffan, L. J. Cooper, M. Naveed, A. G. Bengough, I. Sinclair, P. D. Hallett, T. Roose

Research output: Contribution to journalArticle

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Abstract

In this paper, we provide the first direct evidence of the importance of root hairs on pore structure development at the root-soil interface during the early stage of crop establishment. This was achieved by use of high resolution (~5 µm) synchrotron radiation computed tomography (SRCT) to visualise both the structure of root hairs and the soil pore structure in plant-soil microcosms. Two contrasting genotypes of barley (Hordeum vulgare L.), with and without root hairs, were grown for 8 days in microcosms packedwith sandy loam soil at 1.2 g cm-3 35 dry bulk density. Root hairs were visualised within air filled pore spaces, but not in the fine-textured soil regions.  We found that the genotype with root hairs significantly altered the porosity and connectivity of the detectable pore space (> 5 µm) in the rhizosphere, as compared with the no-hair mutants. Both genotypes showed decreasing pore-space between 0.8 mm and 0.1 mm from the root surface. Interestingly the root-hair-bearing genotype had a significantly greater soil pore volume-fraction at the root-soil interface. Effects of pore structure on diffusion and permeability were estimated to be functionally insignificant under saturated conditions when simulated using image based modelling.
Original languageEnglish
Pages (from-to)124-135
Number of pages11
JournalNew Phytologist
Volume216
Issue number1
Early online date31 Jul 2017
DOIs
Publication statusPublished - Oct 2017

Fingerprint

Rhizosphere
Synchrotrons
root hairs
soil structure
rhizosphere
Soil
image analysis
Genotype
soil pore system
genotype
Hordeum
saturated conditions
fine-textured soils
soil
plant establishment
Plant Structures
sandy loam soils
computed tomography
Porosity
trichomes

Keywords

  • image-based modelling
  • non-invasive imaging
  • rhizosphere
  • root hairs
  • soil structure
  • synchroton

Cite this

Koebernik, N., Daly, K. R., Keyes, S. D., George, T. S., Brown, L. . K., Raffan, A., ... Roose, T. (2017). High-resolution synchrotron imaging shows that root hairs influence rhizosphere soil structure formation. New Phytologist, 216(1), 124-135. https://doi.org/10.1111/nph.14705

High-resolution synchrotron imaging shows that root hairs influence rhizosphere soil structure formation. / Koebernik, N.; Daly, K. R.; Keyes, S. D.; George, T. S.; Brown, L .K.; Raffan, A.; Cooper, L. J.; Naveed, M.; Bengough, A. G.; Sinclair, I.; Hallett, P. D.; Roose, T.

In: New Phytologist, Vol. 216, No. 1, 10.2017, p. 124-135.

Research output: Contribution to journalArticle

Koebernik, N, Daly, KR, Keyes, SD, George, TS, Brown, LK, Raffan, A, Cooper, LJ, Naveed, M, Bengough, AG, Sinclair, I, Hallett, PD & Roose, T 2017, 'High-resolution synchrotron imaging shows that root hairs influence rhizosphere soil structure formation', New Phytologist, vol. 216, no. 1, pp. 124-135. https://doi.org/10.1111/nph.14705
Koebernik, N. ; Daly, K. R. ; Keyes, S. D. ; George, T. S. ; Brown, L .K. ; Raffan, A. ; Cooper, L. J. ; Naveed, M. ; Bengough, A. G. ; Sinclair, I. ; Hallett, P. D. ; Roose, T. / High-resolution synchrotron imaging shows that root hairs influence rhizosphere soil structure formation. In: New Phytologist. 2017 ; Vol. 216, No. 1. pp. 124-135.
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abstract = "In this paper, we provide the first direct evidence of the importance of root hairs on pore structure development at the root-soil interface during the early stage of crop establishment. This was achieved by use of high resolution (~5 µm) synchrotron radiation computed tomography (SRCT) to visualise both the structure of root hairs and the soil pore structure in plant-soil microcosms. Two contrasting genotypes of barley (Hordeum vulgare L.), with and without root hairs, were grown for 8 days in microcosms packedwith sandy loam soil at 1.2 g cm-3 35 dry bulk density. Root hairs were visualised within air filled pore spaces, but not in the fine-textured soil regions.  We found that the genotype with root hairs significantly altered the porosity and connectivity of the detectable pore space (> 5 µm) in the rhizosphere, as compared with the no-hair mutants. Both genotypes showed decreasing pore-space between 0.8 mm and 0.1 mm from the root surface. Interestingly the root-hair-bearing genotype had a significantly greater soil pore volume-fraction at the root-soil interface. Effects of pore structure on diffusion and permeability were estimated to be functionally insignificant under saturated conditions when simulated using image based modelling.",
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author = "N. Koebernik and Daly, {K. R.} and Keyes, {S. D.} and George, {T. S.} and Brown, {L .K.} and A. Raffan and Cooper, {L. J.} and M. Naveed and Bengough, {A. G.} and I. Sinclair and Hallett, {P. D.} and T. Roose",
note = "Acknowledgements L.J.C., N.K., I.S. and T.R. are funded by BBSRC SARISA BB/L025620/1. K.R.D. is funded by ERC 646809DIMR. L.K.B., P.D.H., T.S.G., M.N. and A.R. are funded by BBSRC BB/J00868/1 and A.G.B. is funded by BB/L025825/1. The James Hutton Institute receives financial support from the Rural & Environment Science & Analytical Services Division of the Scottish Government. I.S. and T.R. are also funded by EPSRC EP/M020355/1. T.R. is also funded by ERC 646809DIMR, BBSRC SARIC BB/P004180/1 and NERC NE/L00237/1. The authors acknowledge the use of the I13 beamline at Diamond Light Source, Oxfordshire, UK (session ID: MT9659). We would like to thank beamline scientist Dr Mirian Garcia Fernandez, who provided considerable help during our beamtime. Additionally, Diamond Light Source funded travel for three people. The authors acknowledge the use of the IRIDIS High Performance Computing Facility, and associated support services at the University of Southampton, in the completion of this work. All data supporting this study are available on request from the University of Southampton repository at https://doi.org/10.5258/soton/d0116.",
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TY - JOUR

T1 - High-resolution synchrotron imaging shows that root hairs influence rhizosphere soil structure formation

AU - Koebernik, N.

AU - Daly, K. R.

AU - Keyes, S. D.

AU - George, T. S.

AU - Brown, L .K.

AU - Raffan, A.

AU - Cooper, L. J.

AU - Naveed, M.

AU - Bengough, A. G.

AU - Sinclair, I.

AU - Hallett, P. D.

AU - Roose, T.

N1 - Acknowledgements L.J.C., N.K., I.S. and T.R. are funded by BBSRC SARISA BB/L025620/1. K.R.D. is funded by ERC 646809DIMR. L.K.B., P.D.H., T.S.G., M.N. and A.R. are funded by BBSRC BB/J00868/1 and A.G.B. is funded by BB/L025825/1. The James Hutton Institute receives financial support from the Rural & Environment Science & Analytical Services Division of the Scottish Government. I.S. and T.R. are also funded by EPSRC EP/M020355/1. T.R. is also funded by ERC 646809DIMR, BBSRC SARIC BB/P004180/1 and NERC NE/L00237/1. The authors acknowledge the use of the I13 beamline at Diamond Light Source, Oxfordshire, UK (session ID: MT9659). We would like to thank beamline scientist Dr Mirian Garcia Fernandez, who provided considerable help during our beamtime. Additionally, Diamond Light Source funded travel for three people. The authors acknowledge the use of the IRIDIS High Performance Computing Facility, and associated support services at the University of Southampton, in the completion of this work. All data supporting this study are available on request from the University of Southampton repository at https://doi.org/10.5258/soton/d0116.

PY - 2017/10

Y1 - 2017/10

N2 - In this paper, we provide the first direct evidence of the importance of root hairs on pore structure development at the root-soil interface during the early stage of crop establishment. This was achieved by use of high resolution (~5 µm) synchrotron radiation computed tomography (SRCT) to visualise both the structure of root hairs and the soil pore structure in plant-soil microcosms. Two contrasting genotypes of barley (Hordeum vulgare L.), with and without root hairs, were grown for 8 days in microcosms packedwith sandy loam soil at 1.2 g cm-3 35 dry bulk density. Root hairs were visualised within air filled pore spaces, but not in the fine-textured soil regions.  We found that the genotype with root hairs significantly altered the porosity and connectivity of the detectable pore space (> 5 µm) in the rhizosphere, as compared with the no-hair mutants. Both genotypes showed decreasing pore-space between 0.8 mm and 0.1 mm from the root surface. Interestingly the root-hair-bearing genotype had a significantly greater soil pore volume-fraction at the root-soil interface. Effects of pore structure on diffusion and permeability were estimated to be functionally insignificant under saturated conditions when simulated using image based modelling.

AB - In this paper, we provide the first direct evidence of the importance of root hairs on pore structure development at the root-soil interface during the early stage of crop establishment. This was achieved by use of high resolution (~5 µm) synchrotron radiation computed tomography (SRCT) to visualise both the structure of root hairs and the soil pore structure in plant-soil microcosms. Two contrasting genotypes of barley (Hordeum vulgare L.), with and without root hairs, were grown for 8 days in microcosms packedwith sandy loam soil at 1.2 g cm-3 35 dry bulk density. Root hairs were visualised within air filled pore spaces, but not in the fine-textured soil regions.  We found that the genotype with root hairs significantly altered the porosity and connectivity of the detectable pore space (> 5 µm) in the rhizosphere, as compared with the no-hair mutants. Both genotypes showed decreasing pore-space between 0.8 mm and 0.1 mm from the root surface. Interestingly the root-hair-bearing genotype had a significantly greater soil pore volume-fraction at the root-soil interface. Effects of pore structure on diffusion and permeability were estimated to be functionally insignificant under saturated conditions when simulated using image based modelling.

KW - image-based modelling

KW - non-invasive imaging

KW - rhizosphere

KW - root hairs

KW - soil structure

KW - synchroton

U2 - 10.1111/nph.14705

DO - 10.1111/nph.14705

M3 - Article

VL - 216

SP - 124

EP - 135

JO - New Phytologist

JF - New Phytologist

SN - 0028-646X

IS - 1

ER -