Higher In vitro Proliferation Rate of Rhizopus oryzae in Blood of Diabetic Individuals in Chronic Glycaemic Control Compared with Non-diabetic Individuals

Grace Salazar-Tamayo, Luis E. López-Jácome, Jesús Resendiz-Sanchez, Rafael Franco-Cendejas, Patricia Rodriguez-Zulueta, Dora E. Corzo Leon

Research output: Contribution to journalArticle

1 Citation (Scopus)
10 Downloads (Pure)

Abstract

Metabolic control improves outcomes associated with mucormycosis. The aim of this study was to compare the in vitro proliferation of Rhizopus oryzae in blood of individuals with and without diabetes at different glycaemic levels. Ninety-five individuals were included. Blood samples from each participant were incubated with sporangiospores of R. oryzae. The germination, filamentation and growth of R. oryzae were compared at different time points. Four groups were defined, one without (group A, n = 30) and three with diabetes: group B (HbA1c ≤7%, N = 24), group C (HbA1c 7.1–9%, N = 20) and group D (HbA1c > 9%, N = 21). The percentage of germinated sporangiospores was higher in the group A after 6 h (group A 56% ± 3, group B 35% ± 4, group C 48% ± 4, group D 46% ± 1.4, p = 0.01), 12 h (group A 54% ± 1.4, group B 19% ± 4, group C 16% ± 1, group D 9.5% ± 5, p < 0.001) and 24 h (group A 29% ± 1, group B 12% ± 4, group C 13.5% ± 3.5, group D 12% ± 1, p < 0.01). The filamentation was higher in groups with diabetes. Group B showed higher filamentation grade than group A at 6 h (0.4 ± 0.04 vs 1 ± 0.09, p < 0.001) and 24 h (1.6 ± 0.05 vs 2.1 ± 0.1, p = 0.05). In conclusion, R. oryzae proliferation was higher among diabetic individuals, including good glycaemic control, than among non-diabetic individuals.
Original languageEnglish
Pages (from-to)1005-1014
Number of pages11
JournalMycopathologia
Volume182
Issue number11-12
Early online date6 Jul 2017
DOIs
Publication statusPublished - Dec 2017

Fingerprint

Rhizopus oryzae
Rhizopus
glycemic control
glycohemoglobin
diabetes
blood
Mucormycosis
N,N-dimethyl-3,3-diphenyl-1-methylallylamine
Germination
germination
Oryza
In Vitro Techniques
Growth
sampling

Keywords

  • Mucormycosis
  • diabetes
  • proliferation
  • germination
  • Rhizopus oryzae

Cite this

Salazar-Tamayo, G., López-Jácome, L. E., Resendiz-Sanchez, J., Franco-Cendejas, R., Rodriguez-Zulueta, P., & Corzo Leon, D. E. (2017). Higher In vitro Proliferation Rate of Rhizopus oryzae in Blood of Diabetic Individuals in Chronic Glycaemic Control Compared with Non-diabetic Individuals. Mycopathologia, 182(11-12), 1005-1014. https://doi.org/10.1007/s11046-017-0174-0

Higher In vitro Proliferation Rate of Rhizopus oryzae in Blood of Diabetic Individuals in Chronic Glycaemic Control Compared with Non-diabetic Individuals. / Salazar-Tamayo, Grace ; López-Jácome, Luis E. ; Resendiz-Sanchez, Jesús ; Franco-Cendejas, Rafael ; Rodriguez-Zulueta, Patricia; Corzo Leon, Dora E.

In: Mycopathologia, Vol. 182, No. 11-12, 12.2017, p. 1005-1014.

Research output: Contribution to journalArticle

Salazar-Tamayo, G, López-Jácome, LE, Resendiz-Sanchez, J, Franco-Cendejas, R, Rodriguez-Zulueta, P & Corzo Leon, DE 2017, 'Higher In vitro Proliferation Rate of Rhizopus oryzae in Blood of Diabetic Individuals in Chronic Glycaemic Control Compared with Non-diabetic Individuals', Mycopathologia, vol. 182, no. 11-12, pp. 1005-1014. https://doi.org/10.1007/s11046-017-0174-0
Salazar-Tamayo G, López-Jácome LE, Resendiz-Sanchez J, Franco-Cendejas R, Rodriguez-Zulueta P, Corzo Leon DE. Higher In vitro Proliferation Rate of Rhizopus oryzae in Blood of Diabetic Individuals in Chronic Glycaemic Control Compared with Non-diabetic Individuals. Mycopathologia. 2017 Dec;182(11-12):1005-1014. https://doi.org/10.1007/s11046-017-0174-0
Salazar-Tamayo, Grace ; López-Jácome, Luis E. ; Resendiz-Sanchez, Jesús ; Franco-Cendejas, Rafael ; Rodriguez-Zulueta, Patricia ; Corzo Leon, Dora E. / Higher In vitro Proliferation Rate of Rhizopus oryzae in Blood of Diabetic Individuals in Chronic Glycaemic Control Compared with Non-diabetic Individuals. In: Mycopathologia. 2017 ; Vol. 182, No. 11-12. pp. 1005-1014.
@article{16ea5653d9574ff6afbefa912eb627a9,
title = "Higher In vitro Proliferation Rate of Rhizopus oryzae in Blood of Diabetic Individuals in Chronic Glycaemic Control Compared with Non-diabetic Individuals",
abstract = "Metabolic control improves outcomes associated with mucormycosis. The aim of this study was to compare the in vitro proliferation of Rhizopus oryzae in blood of individuals with and without diabetes at different glycaemic levels. Ninety-five individuals were included. Blood samples from each participant were incubated with sporangiospores of R. oryzae. The germination, filamentation and growth of R. oryzae were compared at different time points. Four groups were defined, one without (group A, n = 30) and three with diabetes: group B (HbA1c ≤7{\%}, N = 24), group C (HbA1c 7.1–9{\%}, N = 20) and group D (HbA1c > 9{\%}, N = 21). The percentage of germinated sporangiospores was higher in the group A after 6 h (group A 56{\%} ± 3, group B 35{\%} ± 4, group C 48{\%} ± 4, group D 46{\%} ± 1.4, p = 0.01), 12 h (group A 54{\%} ± 1.4, group B 19{\%} ± 4, group C 16{\%} ± 1, group D 9.5{\%} ± 5, p < 0.001) and 24 h (group A 29{\%} ± 1, group B 12{\%} ± 4, group C 13.5{\%} ± 3.5, group D 12{\%} ± 1, p < 0.01). The filamentation was higher in groups with diabetes. Group B showed higher filamentation grade than group A at 6 h (0.4 ± 0.04 vs 1 ± 0.09, p < 0.001) and 24 h (1.6 ± 0.05 vs 2.1 ± 0.1, p = 0.05). In conclusion, R. oryzae proliferation was higher among diabetic individuals, including good glycaemic control, than among non-diabetic individuals.",
keywords = "Mucormycosis, diabetes, proliferation, germination, Rhizopus oryzae",
author = "Grace Salazar-Tamayo and L{\'o}pez-J{\'a}come, {Luis E.} and Jes{\'u}s Resendiz-Sanchez and Rafael Franco-Cendejas and Patricia Rodriguez-Zulueta and {Corzo Leon}, {Dora E.}",
note = "We thank all members of the Laboratory of Clinical Microbiology in the Hospital General Dr. Manuel Gea Gonz{\'a}lez, Instituto Nacional de Rehabilitacion and Hospital General de M{\'e}xico. Also, thanks to the Wellcome Trust Strategic Award, corresponding author’s scholarship sponsor. Financial Support This study did not have pharmaceutical or grant support, and resources were obtained from institutional budgets.",
year = "2017",
month = "12",
doi = "10.1007/s11046-017-0174-0",
language = "English",
volume = "182",
pages = "1005--1014",
journal = "Mycopathologia",
issn = "0301-486X",
publisher = "Springer Netherlands",
number = "11-12",

}

TY - JOUR

T1 - Higher In vitro Proliferation Rate of Rhizopus oryzae in Blood of Diabetic Individuals in Chronic Glycaemic Control Compared with Non-diabetic Individuals

AU - Salazar-Tamayo, Grace

AU - López-Jácome, Luis E.

AU - Resendiz-Sanchez, Jesús

AU - Franco-Cendejas, Rafael

AU - Rodriguez-Zulueta, Patricia

AU - Corzo Leon, Dora E.

N1 - We thank all members of the Laboratory of Clinical Microbiology in the Hospital General Dr. Manuel Gea González, Instituto Nacional de Rehabilitacion and Hospital General de México. Also, thanks to the Wellcome Trust Strategic Award, corresponding author’s scholarship sponsor. Financial Support This study did not have pharmaceutical or grant support, and resources were obtained from institutional budgets.

PY - 2017/12

Y1 - 2017/12

N2 - Metabolic control improves outcomes associated with mucormycosis. The aim of this study was to compare the in vitro proliferation of Rhizopus oryzae in blood of individuals with and without diabetes at different glycaemic levels. Ninety-five individuals were included. Blood samples from each participant were incubated with sporangiospores of R. oryzae. The germination, filamentation and growth of R. oryzae were compared at different time points. Four groups were defined, one without (group A, n = 30) and three with diabetes: group B (HbA1c ≤7%, N = 24), group C (HbA1c 7.1–9%, N = 20) and group D (HbA1c > 9%, N = 21). The percentage of germinated sporangiospores was higher in the group A after 6 h (group A 56% ± 3, group B 35% ± 4, group C 48% ± 4, group D 46% ± 1.4, p = 0.01), 12 h (group A 54% ± 1.4, group B 19% ± 4, group C 16% ± 1, group D 9.5% ± 5, p < 0.001) and 24 h (group A 29% ± 1, group B 12% ± 4, group C 13.5% ± 3.5, group D 12% ± 1, p < 0.01). The filamentation was higher in groups with diabetes. Group B showed higher filamentation grade than group A at 6 h (0.4 ± 0.04 vs 1 ± 0.09, p < 0.001) and 24 h (1.6 ± 0.05 vs 2.1 ± 0.1, p = 0.05). In conclusion, R. oryzae proliferation was higher among diabetic individuals, including good glycaemic control, than among non-diabetic individuals.

AB - Metabolic control improves outcomes associated with mucormycosis. The aim of this study was to compare the in vitro proliferation of Rhizopus oryzae in blood of individuals with and without diabetes at different glycaemic levels. Ninety-five individuals were included. Blood samples from each participant were incubated with sporangiospores of R. oryzae. The germination, filamentation and growth of R. oryzae were compared at different time points. Four groups were defined, one without (group A, n = 30) and three with diabetes: group B (HbA1c ≤7%, N = 24), group C (HbA1c 7.1–9%, N = 20) and group D (HbA1c > 9%, N = 21). The percentage of germinated sporangiospores was higher in the group A after 6 h (group A 56% ± 3, group B 35% ± 4, group C 48% ± 4, group D 46% ± 1.4, p = 0.01), 12 h (group A 54% ± 1.4, group B 19% ± 4, group C 16% ± 1, group D 9.5% ± 5, p < 0.001) and 24 h (group A 29% ± 1, group B 12% ± 4, group C 13.5% ± 3.5, group D 12% ± 1, p < 0.01). The filamentation was higher in groups with diabetes. Group B showed higher filamentation grade than group A at 6 h (0.4 ± 0.04 vs 1 ± 0.09, p < 0.001) and 24 h (1.6 ± 0.05 vs 2.1 ± 0.1, p = 0.05). In conclusion, R. oryzae proliferation was higher among diabetic individuals, including good glycaemic control, than among non-diabetic individuals.

KW - Mucormycosis

KW - diabetes

KW - proliferation

KW - germination

KW - Rhizopus oryzae

U2 - 10.1007/s11046-017-0174-0

DO - 10.1007/s11046-017-0174-0

M3 - Article

VL - 182

SP - 1005

EP - 1014

JO - Mycopathologia

JF - Mycopathologia

SN - 0301-486X

IS - 11-12

ER -