Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 1005-1014 |
| Number of pages | 11 |
| Journal | Mycopathologia |
| Volume | 182 |
| Issue number | 11-12 |
| Early online date | 6 Jul 2017 |
| DOIs | |
| Publication status | Published - Dec 2017 |
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Keywords
- Mucormycosis
- diabetes
- proliferation
- germination
- Rhizopus oryzae
Cite this
Higher In vitro Proliferation Rate of Rhizopus oryzae in Blood of Diabetic Individuals in Chronic Glycaemic Control Compared with Non-diabetic Individuals. / Salazar-Tamayo, Grace ; López-Jácome, Luis E. ; Resendiz-Sanchez, Jesús ; Franco-Cendejas, Rafael ; Rodriguez-Zulueta, Patricia; Corzo Leon, Dora E.
In: Mycopathologia, Vol. 182, No. 11-12, 12.2017, p. 1005-1014.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Higher In vitro Proliferation Rate of Rhizopus oryzae in Blood of Diabetic Individuals in Chronic Glycaemic Control Compared with Non-diabetic Individuals
AU - Salazar-Tamayo, Grace
AU - López-Jácome, Luis E.
AU - Resendiz-Sanchez, Jesús
AU - Franco-Cendejas, Rafael
AU - Rodriguez-Zulueta, Patricia
AU - Corzo Leon, Dora E.
N1 - We thank all members of the Laboratory of Clinical Microbiology in the Hospital General Dr. Manuel Gea González, Instituto Nacional de Rehabilitacion and Hospital General de México. Also, thanks to the Wellcome Trust Strategic Award, corresponding author’s scholarship sponsor. Financial Support This study did not have pharmaceutical or grant support, and resources were obtained from institutional budgets.
PY - 2017/12
Y1 - 2017/12
N2 - Metabolic control improves outcomes associated with mucormycosis. The aim of this study was to compare the in vitro proliferation of Rhizopus oryzae in blood of individuals with and without diabetes at different glycaemic levels. Ninety-five individuals were included. Blood samples from each participant were incubated with sporangiospores of R. oryzae. The germination, filamentation and growth of R. oryzae were compared at different time points. Four groups were defined, one without (group A, n = 30) and three with diabetes: group B (HbA1c ≤7%, N = 24), group C (HbA1c 7.1–9%, N = 20) and group D (HbA1c > 9%, N = 21). The percentage of germinated sporangiospores was higher in the group A after 6 h (group A 56% ± 3, group B 35% ± 4, group C 48% ± 4, group D 46% ± 1.4, p = 0.01), 12 h (group A 54% ± 1.4, group B 19% ± 4, group C 16% ± 1, group D 9.5% ± 5, p < 0.001) and 24 h (group A 29% ± 1, group B 12% ± 4, group C 13.5% ± 3.5, group D 12% ± 1, p < 0.01). The filamentation was higher in groups with diabetes. Group B showed higher filamentation grade than group A at 6 h (0.4 ± 0.04 vs 1 ± 0.09, p < 0.001) and 24 h (1.6 ± 0.05 vs 2.1 ± 0.1, p = 0.05). In conclusion, R. oryzae proliferation was higher among diabetic individuals, including good glycaemic control, than among non-diabetic individuals.
AB - Metabolic control improves outcomes associated with mucormycosis. The aim of this study was to compare the in vitro proliferation of Rhizopus oryzae in blood of individuals with and without diabetes at different glycaemic levels. Ninety-five individuals were included. Blood samples from each participant were incubated with sporangiospores of R. oryzae. The germination, filamentation and growth of R. oryzae were compared at different time points. Four groups were defined, one without (group A, n = 30) and three with diabetes: group B (HbA1c ≤7%, N = 24), group C (HbA1c 7.1–9%, N = 20) and group D (HbA1c > 9%, N = 21). The percentage of germinated sporangiospores was higher in the group A after 6 h (group A 56% ± 3, group B 35% ± 4, group C 48% ± 4, group D 46% ± 1.4, p = 0.01), 12 h (group A 54% ± 1.4, group B 19% ± 4, group C 16% ± 1, group D 9.5% ± 5, p < 0.001) and 24 h (group A 29% ± 1, group B 12% ± 4, group C 13.5% ± 3.5, group D 12% ± 1, p < 0.01). The filamentation was higher in groups with diabetes. Group B showed higher filamentation grade than group A at 6 h (0.4 ± 0.04 vs 1 ± 0.09, p < 0.001) and 24 h (1.6 ± 0.05 vs 2.1 ± 0.1, p = 0.05). In conclusion, R. oryzae proliferation was higher among diabetic individuals, including good glycaemic control, than among non-diabetic individuals.
KW - Mucormycosis
KW - diabetes
KW - proliferation
KW - germination
KW - Rhizopus oryzae
U2 - 10.1007/s11046-017-0174-0
DO - 10.1007/s11046-017-0174-0
M3 - Article
VL - 182
SP - 1005
EP - 1014
JO - Mycopathologia
JF - Mycopathologia
SN - 0301-486X
IS - 11-12
ER -