Highly conserved DNA sequence present in small plasmids from Selenomonas ruminantium

K  Fliegerova; S  Pazoutova; P  Pristas; H J  Flint

Highly conserved DNA sequence present in small plasmids from Selenomonas ruminantium

K Fliegerova, S Pazoutova, P Pristas, H J Flint

The Rowett Institute of Nutrition and Health

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Plasmid pJW1 from Selenomonas ruminantium subsp. lactilytica strain JW13 has been cloned in Escherichia coli vector pBluescriptSK(-) and completely sequenced. The plasmid is only 1410 bp with an overall GC content of 42.2%. Computer analysis of sequence data revealed a single open reading frame (ORF1, 146 amino acids, MW 16.525.5 Da) encoding a putative replication protein which is similar to the Rep protein of Ruminobacter, amylophilus plasmid pRAO1. ORF1 is followed by a long AT-rich (75%) region and a region abundant in direct and inverted repeats. Comparison of DNA sequences revealed the presence of a short (<250 bp) DNA segment which is highly conserved between several small S. ruminantium plasmids including pJDB21, (C) 2000 Academic Press.

Original language	English
Pages (from-to)	94-99
Number of pages	6
Journal	Plasmid
Volume	44
Issue number	1
Publication status	Published - Jul 2000

Keywords

COMPLETE NUCLEOTIDE-SEQUENCE
SMALL CRYPTIC PLASMIDS
ESCHERICHIA-COLI
REPLICATION
ALIGNMENT
REGION

Cite this

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title = "Highly conserved DNA sequence present in small plasmids from Selenomonas ruminantium",

abstract = "Plasmid pJW1 from Selenomonas ruminantium subsp. lactilytica strain JW13 has been cloned in Escherichia coli vector pBluescriptSK(-) and completely sequenced. The plasmid is only 1410 bp with an overall GC content of 42.2%. Computer analysis of sequence data revealed a single open reading frame (ORF1, 146 amino acids, MW 16.525.5 Da) encoding a putative replication protein which is similar to the Rep protein of Ruminobacter, amylophilus plasmid pRAO1. ORF1 is followed by a long AT-rich (75%) region and a region abundant in direct and inverted repeats. Comparison of DNA sequences revealed the presence of a short (<250 bp) DNA segment which is highly conserved between several small S. ruminantium plasmids including pJDB21, (C) 2000 Academic Press.",

keywords = "COMPLETE NUCLEOTIDE-SEQUENCE, SMALL CRYPTIC PLASMIDS, ESCHERICHIA-COLI, REPLICATION, ALIGNMENT, REGION",

author = "K Fliegerova and S Pazoutova and P Pristas and Flint, {H J}",

year = "2000",

month = jul,

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TY - JOUR

T1 - Highly conserved DNA sequence present in small plasmids from Selenomonas ruminantium

AU - Fliegerova, K

AU - Pazoutova, S

AU - Pristas, P

AU - Flint, H J

PY - 2000/7

Y1 - 2000/7

N2 - Plasmid pJW1 from Selenomonas ruminantium subsp. lactilytica strain JW13 has been cloned in Escherichia coli vector pBluescriptSK(-) and completely sequenced. The plasmid is only 1410 bp with an overall GC content of 42.2%. Computer analysis of sequence data revealed a single open reading frame (ORF1, 146 amino acids, MW 16.525.5 Da) encoding a putative replication protein which is similar to the Rep protein of Ruminobacter, amylophilus plasmid pRAO1. ORF1 is followed by a long AT-rich (75%) region and a region abundant in direct and inverted repeats. Comparison of DNA sequences revealed the presence of a short (<250 bp) DNA segment which is highly conserved between several small S. ruminantium plasmids including pJDB21, (C) 2000 Academic Press.

AB - Plasmid pJW1 from Selenomonas ruminantium subsp. lactilytica strain JW13 has been cloned in Escherichia coli vector pBluescriptSK(-) and completely sequenced. The plasmid is only 1410 bp with an overall GC content of 42.2%. Computer analysis of sequence data revealed a single open reading frame (ORF1, 146 amino acids, MW 16.525.5 Da) encoding a putative replication protein which is similar to the Rep protein of Ruminobacter, amylophilus plasmid pRAO1. ORF1 is followed by a long AT-rich (75%) region and a region abundant in direct and inverted repeats. Comparison of DNA sequences revealed the presence of a short (<250 bp) DNA segment which is highly conserved between several small S. ruminantium plasmids including pJDB21, (C) 2000 Academic Press.

KW - COMPLETE NUCLEOTIDE-SEQUENCE

KW - SMALL CRYPTIC PLASMIDS

KW - ESCHERICHIA-COLI

KW - REPLICATION

KW - ALIGNMENT

KW - REGION

M3 - Article

SN - 0147-619X

VL - 44

SP - 94

EP - 99

JO - Plasmid

JF - Plasmid

IS - 1

ER -

Highly conserved DNA sequence present in small plasmids from Selenomonas ruminantium

Abstract

Keywords

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