HISTOLOGICAL STAINING OF NUCLEAR MATERIAL IN HYPHAE OF STREPTOMYCES-COELICOLOR A3(2)

Derek Gray, G W GOODAY, James Ivor Prosser

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Staining methods involving Giemsa, thionin or azur A were all used to demonstrate the size and distribution of nucleoids within spores and young substrate hyphae of the actinomycete Streptomyces coelicolor A3(2). The significance of observations made upon nucleoids visualised using such histological strains is questionable, because fixation and other preparative steps alter their state in vivo. The appearance of nucleoids in unfixed preparations following fluorescent staining with DAPI, mithramycin, ethidium bromide, Hoechst-33258 or beberine sulphate was similar and more accurately represented the true disposition of nucleoids within hyphae. Flourescent staining of chemically fixed preparations gave results similar to those of histologically stained material of the same age, that were also subject to fixation procedures. Staining young germ tube hyphae with DAPI also revealed the presence of polyphosphate storage granules.

Original languageEnglish
Pages (from-to)163-172
Number of pages10
JournalJournal of Microbiological Methods
Volume12
Issue number3-4
Publication statusPublished - Dec 1990

Keywords

  • NUCLEAR STAINING
  • STREPTOMYCES-COELICOLOR
  • STREPTOMYCETE
  • DNA
  • POLYPHOSPHATES
  • FLUORESCENCE
  • LOCALIZATION
  • MICROSCOPY
  • DAPI

Cite this

HISTOLOGICAL STAINING OF NUCLEAR MATERIAL IN HYPHAE OF STREPTOMYCES-COELICOLOR A3(2). / Gray, Derek; GOODAY, G W ; Prosser, James Ivor.

In: Journal of Microbiological Methods, Vol. 12, No. 3-4, 12.1990, p. 163-172.

Research output: Contribution to journalArticle

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