Holmium as a faecal marker for copper absorption studies in adults

L J Harvey, G Majsak-Newman, J R Dainty, S G Wharf, Martin David Reid, John Hamilton Beattie, S J Fairweather-Tait

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    Abstract

    The objective of the present study was to investigate the validity of using holmiurn as a faecal marker in a copper absorption study using a highly enriched Cu-65 stable isotope label. Ten volunteers (nine female, one male) aged 24-55 years were recruited from the Norwich area to take part in a free-living study. The study was conducted in the Human Nutrition Unit at the Institute of Food Research, Norwich, U.K., and involved feeding breakfast test meals containing foods labelled intrinsically or extrinsically with a highly enriched Cu-65 stable isotope label. A 1 mg oral dose of the rare earth element holmiurn was administered to volunteers simultaneously with an oral dose of highly enriched Cu-65 stable isotope as a label in the breakfast test meal. Complete faecal collections were made for 10 days after dosing, and the mean percentage holmium recovery (+/-S.D.) was 105+/-15%. After correcting for re-excreted copper label, the labelled copper and holmium had similar excretory profiles. It was concluded that holmium is a valid faecal marker in adult human copper absorption studies using stable isotopes.

    Original languageEnglish
    Pages (from-to)233-240
    Number of pages8
    JournalClinical Science
    Volume102
    Issue number2
    Publication statusPublished - Feb 2002

    Keywords

    • adults
    • copper absorption
    • holmium
    • rare earth element
    • stable isotope
    • tracers
    • iron

    Cite this

    Harvey, L. J., Majsak-Newman, G., Dainty, J. R., Wharf, S. G., Reid, M. D., Beattie, J. H., & Fairweather-Tait, S. J. (2002). Holmium as a faecal marker for copper absorption studies in adults. Clinical Science, 102(2), 233-240.