Host carbon sources modulate cell wall architecture, drug resistance and virulence in a fungal pathogen

Iuliana Veronica Ene, Ashok Adya, Silvia Wehmeier, Alex Brand, Donna Margaret MacCallum, Neil Andrew Robert Gow, Alistair James Petersen Brown

Research output: Contribution to journalArticle

118 Citations (Scopus)
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Abstract

The survival of all microbes depends upon their ability to respond to environmental challenges. To establish infection, pathogens such as Candida albicans must mount effective stress responses to counter host defences while adapting to dynamic changes in nutrient status within host niches. Studies of C.¿albicans stress adaptation have generally been performed on glucose-grown cells, leaving the effects of alternative carbon sources upon stress resistance largely unexplored. We have shown that growth on alternative carbon sources, such as lactate, strongly influence the resistance of C.¿albicans to antifungal drugs, osmotic and cell wall stresses. Similar trends were observed in clinical isolates and other pathogenic Candida species. The increased stress resistance of C.¿albicans was not dependent on key stress (Hog1) and cell integrity (Mkc1) signalling pathways. Instead, increased stress resistance was promoted by major changes in the architecture and biophysical properties of the cell wall. Glucose- and lactate-grown cells displayed significant differences in cell wall mass, ultrastructure, elasticity and adhesion. Changes in carbon source also altered the virulence of C.¿albicans in models of systemic candidiasis and vaginitis, confirming the importance of alternative carbon sources within host niches during C.¿albicans infections.
Original languageEnglish
Pages (from-to)1319-1335
Number of pages17
JournalCellular Microbiology
Volume14
Issue number9
Early online date5 Jun 2012
DOIs
Publication statusPublished - Sep 2012

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Drug Resistance
Cell Wall
Virulence
Carbon
Lactic Acid
Vaginitis
Glucose
Elasticity
Infection
Candida albicans
Candida
Food
Growth
Pharmaceutical Preparations

Cite this

Host carbon sources modulate cell wall architecture, drug resistance and virulence in a fungal pathogen. / Ene, Iuliana Veronica; Adya, Ashok; Wehmeier, Silvia; Brand, Alex; MacCallum, Donna Margaret; Gow, Neil Andrew Robert; Brown, Alistair James Petersen.

In: Cellular Microbiology, Vol. 14, No. 9, 09.2012, p. 1319-1335.

Research output: Contribution to journalArticle

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