Host-targeting protein 1 (SpHtp1) from the oomycete Saprolegnia parasitica translocates specifically into fish cells in a tyrosine-O-sulphate-dependent manner

Stephan Werner Wawra, Judith Margaret Bain, E Durward, I de Bruijn, KL Minor, A Matena, L Lobach, SC Whisson, P Bayer, Andrew Justin Radcliffe Porter, Paul Birch, Christopher John Secombes, Pieter Van West

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The eukaryotic oomycetes, or water molds, contain several species that are devastating pathogens of plants and animals. During infection, oomycetes translocate effector proteins into host cells, where they interfere with host-defense responses. For several oomycete effectors (i.e., the RxLR-effectors) it has been shown that their N-terminal polypeptides are important for the delivery into the host. Here we demonstrate that the putative RxLR-like effector, host-targeting protein 1 (SpHtp1), from the fish pathogen Saprolegnia parasitica translocates specifically inside host cells. We further demonstrate that cell-surface binding and uptake of this effector protein is mediated by an interaction with tyrosine-O-sulfate-modified cell-surface molecules and not via phospholipids, as has been reported for RxLR-effectors from plant pathogenic oomycetes. These results reveal an effector translocation route based on tyrosine-O-sulfate binding, which could be highly relevant for a wide range of host-microbe interactions.
Original languageEnglish
Pages (from-to)2096-2101
Number of pages6
JournalPNAS
Volume109
Issue number6
Early online date20 Jan 2012
DOIs
Publication statusPublished - 7 Feb 2012

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Saprolegnia
Oomycetes
Protein Transport
Fishes
Phospholipids
Proteins
Fungi
Peptides
tyrosine O-sulfate
Water
Infection

Keywords

  • protein translocation
  • phytophthora
  • plasmodium

Cite this

Host-targeting protein 1 (SpHtp1) from the oomycete Saprolegnia parasitica translocates specifically into fish cells in a tyrosine-O-sulphate-dependent manner. / Wawra, Stephan Werner; Bain, Judith Margaret; Durward, E; de Bruijn, I; Minor, KL; Matena, A; Lobach, L; Whisson, SC; Bayer, P; Porter, Andrew Justin Radcliffe; Birch, Paul; Secombes, Christopher John; Van West, Pieter.

In: PNAS, Vol. 109, No. 6, 07.02.2012, p. 2096-2101.

Research output: Contribution to journalArticle

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