Abstract
The eukaryotic oomycetes, or water molds, contain several species that are devastating pathogens of plants and animals. During infection, oomycetes translocate effector proteins into host cells, where they interfere with host-defense responses. For several oomycete effectors (i.e., the RxLR-effectors) it has been shown that their N-terminal polypeptides are important for the delivery into the host. Here we demonstrate that the putative RxLR-like effector, host-targeting protein 1 (SpHtp1), from the fish pathogen Saprolegnia parasitica translocates specifically inside host cells. We further demonstrate that cell-surface binding and uptake of this effector protein is mediated by an interaction with tyrosine-O-sulfate-modified cell-surface molecules and not via phospholipids, as has been reported for RxLR-effectors from plant pathogenic oomycetes. These results reveal an effector translocation route based on tyrosine-O-sulfate binding, which could be highly relevant for a wide range of host-microbe interactions.
Original language | English |
---|---|
Pages (from-to) | 2096-2101 |
Number of pages | 6 |
Journal | PNAS |
Volume | 109 |
Issue number | 6 |
Early online date | 20 Jan 2012 |
DOIs | |
Publication status | Published - 7 Feb 2012 |
Bibliographical note
A paid open access option is available for this journal.Applies to PNAS only
On author's personal website, institutional repository or preprint servers only
Publisher copyright must be acknowledged
Must link to publisher version
Publisher's version/PDF cannot be used in repository, but may be used on authors personal website or authors institutional profile website
Authors may have deposit in funding body archive or designated repository for public release 6 months after publication or immediately upon payment of fee
All PNAS-formatted versions are deposited in PubMed Central for release after 6 months, regardless of funding
Keywords
- protein translocation
- phytophthora
- plasmodium
Fingerprint
Dive into the research topics of 'Host-targeting protein 1 (SpHtp1) from the oomycete Saprolegnia parasitica translocates specifically into fish cells in a tyrosine-O-sulphate-dependent manner'. Together they form a unique fingerprint.Press/Media
-
Route parasite takes to infect fish uncovered
Pieter van West & Stephan Werner Wawra
24/01/12
1 item of Media coverage
Press/Media: Research