How to measure mitochondrial function in birds using red blood cells

a case study in the king penguin and perspectives in ecology and evolution

Antoine Stier, Caroline Romestaing, Quentin Schull, Emilie Lefol, Jean-Patrice Robin, Damien Roussel, Pierre Bize

Research output: Contribution to journalArticle

9 Citations (Scopus)
7 Downloads (Pure)

Abstract

Mitochondria are the powerhouse of animal cells. They produce through oxidative phosphorylation more than 90% of the cellular energy (ATP) required for organism's growth, reproduction, and maintenance. Hence, information on mitochondrial function is expected to bring important insights in animal ecology and evolution. Unfortunately, the invasiveness of the procedures required to measure mitochondrial function (e.g. sampling of liver or muscles) has limited its study in wild vertebrate populations so far. Here, we capitalize on the fact that bird red blood cells (RBCs) possess functional mitochondria to describe a minimally invasive approach to study mitochondrial function, using blood samples.

In the king penguin, we present a protocol, using a high-resolution respirometry system and specific agonists and antagonists enabling the assessment of mitochondrial function in RBCs. We evaluated the inter-assay repeatability of our measures of mitochondrial function, and tested the influence of sample storage and bird handling time on these measures. We also compared measures of mitochondrial function in RBCs and in the pectoral muscle obtained from the same individuals.

Mitochondria from RBCs showed the expected responses to mitochondrial agonists and antagonists, and therefore the protocol presented allows computing effective measures of mitochondrial function. The different measures of RBC mitochondrial function were significantly repeatable, were not affected by the handling time of the bird prior to blood sampling (i.e. stress response), and only minimally affected by the storage time of the sample at 4 °C up to 24 h. Most notably, we showed that mitochondrial parameters measured in RBCs moderately correlated to those measured in the pectoral muscle.

This study sheds light on the use of RBCs in birds as a valuable and minimally invasive source of information on mitochondrial function. This approach opens new opportunities to study mitochondrial function in free-living animals and could bring knowledge gains in ecology and evolution. Fish, amphibians and reptiles also possess mitochondria in their RBCs, and the approach presented here could also be applicable to these taxa.
Original languageEnglish
Pages (from-to)1172-1182
Number of pages11
JournalMethods in Ecology and Evolution
Volume8
Issue number10
Early online date13 Feb 2017
DOIs
Publication statusPublished - Oct 2017

Fingerprint

Spheniscidae
Ecology
Birds
erythrocytes
Erythrocytes
blood
case studies
ecology
bird
birds
mitochondrion
Mitochondria
mitochondria
Pectoralis Muscles
handling time
muscle
muscles
agonists
antagonists
sampling

Keywords

  • mitochondria
  • erythrocyte
  • non-invasive methodology
  • high-resolution respirometry
  • metabolism

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Environmental Science(all)

Cite this

How to measure mitochondrial function in birds using red blood cells : a case study in the king penguin and perspectives in ecology and evolution. / Stier, Antoine; Romestaing, Caroline ; Schull, Quentin; Lefol, Emilie ; Robin, Jean-Patrice; Roussel, Damien; Bize, Pierre.

In: Methods in Ecology and Evolution, Vol. 8, No. 10, 10.2017, p. 1172-1182.

Research output: Contribution to journalArticle

Stier, Antoine ; Romestaing, Caroline ; Schull, Quentin ; Lefol, Emilie ; Robin, Jean-Patrice ; Roussel, Damien ; Bize, Pierre. / How to measure mitochondrial function in birds using red blood cells : a case study in the king penguin and perspectives in ecology and evolution. In: Methods in Ecology and Evolution. 2017 ; Vol. 8, No. 10. pp. 1172-1182.
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