Hsp21potentiates antifungal drug tolerance in Candida albicans

François L Mayer, Duncan Wilson, Bernhard Hube

Research output: Contribution to journalArticle

8 Citations (Scopus)
4 Downloads (Pure)

Abstract

Systemic infections of humans with the fungal pathogen Candida albicans are associated with a high mortality rate. Currently, efficient treatment of these infections is hampered by the relatively low number of available antifungal drugs. We recently identified the small heat shock protein Hsp21 in C. albicans and demonstrated its fundamental role for environmental stress adaptation and fungal virulence. Hsp21 was found in several pathogenic Candida species but not in humans. This prompted us to investigate the effects of a broad range of different antifungal drugs on an Hsp21-null C. albicans mutant strain. Our results indicate that combinatorial therapy targeting Hsp21, together with specific antifungal drug targets, has strong synergistic potential. In addition, we demonstrate that Hsp21 is required for tolerance to ethanol-induced stress and induction of filamentation in response to pharmacological inhibition of Hsp90. These findings might pave the way for the development of new treatment strategies against Candida infections.
Original languageEnglish
Article numbere60417
Number of pages8
JournalPloS ONE
Volume8
Issue number3
DOIs
Publication statusPublished - 22 Mar 2013

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Drug Tolerance
antifungal agents
Candida
drug resistance
Candida albicans
Infection
Pharmaceutical Preparations
Small Heat-Shock Proteins
candidiasis
Pathogens
heat shock proteins
infection
Virulence
Ethanol
virulence
ethanol
Pharmacology
mutants
therapeutics
Mortality

Keywords

  • amphotericin B
  • antifungal agents
  • Candida albicans
  • drug resistance, fungal
  • fungal proteins
  • HSP90 heat-shock proteins
  • heat-shock proteins
  • humans
  • phylogeny

Cite this

Hsp21potentiates antifungal drug tolerance in Candida albicans. / Mayer, François L; Wilson, Duncan; Hube, Bernhard.

In: PloS ONE, Vol. 8, No. 3, e60417, 22.03.2013.

Research output: Contribution to journalArticle

Mayer, François L ; Wilson, Duncan ; Hube, Bernhard. / Hsp21potentiates antifungal drug tolerance in Candida albicans. In: PloS ONE. 2013 ; Vol. 8, No. 3.
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