Human alveolar epithelial cells engulf apoptotic eosinophils by means of integrin- and phosphatidylserine receptor-dependent mechanisms

a process upregulated by dexamethasone

Darren W. Sexton, Morgan G. Blaylock, Garry M. Walsh

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Background: We previously demonstrated receptor-mediated apoptotic-eosinophil engulfment by human small airway epithelial cells, which may represent a potentially important mechanism in the resolution of allergic and asthmatic inflammation.

Objective: A549 cells were selected as being representative of alveolar epithelial cells, and their ability to ingest human apoptotic eosinophils was examined in terms of the effects of dexamethasone treatment and the receptor-mediated recognition mechanisms important in this process.

Methods: A549 epithelial-cell expression of alphav beta3, alphav beta5, CD36, and the phosphatidylserine receptor was established by using immunostaining and flow cytometry, and inhibition assays were examined by using the role of these receptors in apoptotic-eosinophil recognition by resting and dexamethasone-treated A549 epithelial cells. Electron microscopy confirmed engulfment of apoptotic eosinophils, and receptor clustering around apoptotic eosinophils was examined by using immunofluorescent labeling.

Results: A549 epithelial cells recognized and engulfed apoptotic eosinophils but not freshly isolated cells. Dexamethasone enhanced the number of A549 cells ingesting apoptotic eosinophils and dose dependently increased their capacity for ingestion. The tetrapeptide RGDS significantly inhibited apoptotic-eosinophil uptake by A549 cells, indicating a role for integrins in the recognition process. A549 cells expressed alphav beta3, alphav beta5, beta5, CD36, and the phosphatidylserine receptor, and expression of these receptors was not significantly increased after dexamethasone treatment. Engulfment of apoptotic eosinophils by A549 cells involved alphav beta3-, CD36-, alphav beta5-, and phosphatidylserine receptor-mediated recognition mechanisms and was associated with the formation of integrin focal adhesion complexes around apoptotic eosinophils.

Conclusions: These data further suggest a nonpassive role for airway epithelium in the resolution of eosinophilic inflammation in asthma.

Original languageEnglish
Pages (from-to)962-969
Number of pages7
JournalJournal of Allergy and Clinical Immunology
Volume108
Issue number6
DOIs
Publication statusPublished - Dec 2001

Keywords

  • epithelial cells
  • eosinophils
  • apoptosis
  • asthma
  • phagocytic recognition
  • corticosteroids
  • inflammatory cells
  • dendritic cells
  • T-lymphocytes
  • in-vitro
  • phagocytosis
  • recognition
  • alpha(V)beta(5)
  • granulocytes

Cite this

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title = "Human alveolar epithelial cells engulf apoptotic eosinophils by means of integrin- and phosphatidylserine receptor-dependent mechanisms: a process upregulated by dexamethasone",
abstract = "Background: We previously demonstrated receptor-mediated apoptotic-eosinophil engulfment by human small airway epithelial cells, which may represent a potentially important mechanism in the resolution of allergic and asthmatic inflammation.Objective: A549 cells were selected as being representative of alveolar epithelial cells, and their ability to ingest human apoptotic eosinophils was examined in terms of the effects of dexamethasone treatment and the receptor-mediated recognition mechanisms important in this process.Methods: A549 epithelial-cell expression of alphav beta3, alphav beta5, CD36, and the phosphatidylserine receptor was established by using immunostaining and flow cytometry, and inhibition assays were examined by using the role of these receptors in apoptotic-eosinophil recognition by resting and dexamethasone-treated A549 epithelial cells. Electron microscopy confirmed engulfment of apoptotic eosinophils, and receptor clustering around apoptotic eosinophils was examined by using immunofluorescent labeling.Results: A549 epithelial cells recognized and engulfed apoptotic eosinophils but not freshly isolated cells. Dexamethasone enhanced the number of A549 cells ingesting apoptotic eosinophils and dose dependently increased their capacity for ingestion. The tetrapeptide RGDS significantly inhibited apoptotic-eosinophil uptake by A549 cells, indicating a role for integrins in the recognition process. A549 cells expressed alphav beta3, alphav beta5, beta5, CD36, and the phosphatidylserine receptor, and expression of these receptors was not significantly increased after dexamethasone treatment. Engulfment of apoptotic eosinophils by A549 cells involved alphav beta3-, CD36-, alphav beta5-, and phosphatidylserine receptor-mediated recognition mechanisms and was associated with the formation of integrin focal adhesion complexes around apoptotic eosinophils.Conclusions: These data further suggest a nonpassive role for airway epithelium in the resolution of eosinophilic inflammation in asthma.",
keywords = "epithelial cells, eosinophils, apoptosis, asthma, phagocytic recognition, corticosteroids, inflammatory cells, dendritic cells, T-lymphocytes, in-vitro, phagocytosis, recognition, alpha(V)beta(5), granulocytes",
author = "Sexton, {Darren W.} and Blaylock, {Morgan G.} and Walsh, {Garry M.}",
year = "2001",
month = "12",
doi = "10.1067/mai.2001.119414",
language = "English",
volume = "108",
pages = "962--969",
journal = "Journal of Allergy and Clinical Immunology",
issn = "0091-6749",
publisher = "Mosby/Elsevier,",
number = "6",

}

TY - JOUR

T1 - Human alveolar epithelial cells engulf apoptotic eosinophils by means of integrin- and phosphatidylserine receptor-dependent mechanisms

T2 - a process upregulated by dexamethasone

AU - Sexton, Darren W.

AU - Blaylock, Morgan G.

AU - Walsh, Garry M.

PY - 2001/12

Y1 - 2001/12

N2 - Background: We previously demonstrated receptor-mediated apoptotic-eosinophil engulfment by human small airway epithelial cells, which may represent a potentially important mechanism in the resolution of allergic and asthmatic inflammation.Objective: A549 cells were selected as being representative of alveolar epithelial cells, and their ability to ingest human apoptotic eosinophils was examined in terms of the effects of dexamethasone treatment and the receptor-mediated recognition mechanisms important in this process.Methods: A549 epithelial-cell expression of alphav beta3, alphav beta5, CD36, and the phosphatidylserine receptor was established by using immunostaining and flow cytometry, and inhibition assays were examined by using the role of these receptors in apoptotic-eosinophil recognition by resting and dexamethasone-treated A549 epithelial cells. Electron microscopy confirmed engulfment of apoptotic eosinophils, and receptor clustering around apoptotic eosinophils was examined by using immunofluorescent labeling.Results: A549 epithelial cells recognized and engulfed apoptotic eosinophils but not freshly isolated cells. Dexamethasone enhanced the number of A549 cells ingesting apoptotic eosinophils and dose dependently increased their capacity for ingestion. The tetrapeptide RGDS significantly inhibited apoptotic-eosinophil uptake by A549 cells, indicating a role for integrins in the recognition process. A549 cells expressed alphav beta3, alphav beta5, beta5, CD36, and the phosphatidylserine receptor, and expression of these receptors was not significantly increased after dexamethasone treatment. Engulfment of apoptotic eosinophils by A549 cells involved alphav beta3-, CD36-, alphav beta5-, and phosphatidylserine receptor-mediated recognition mechanisms and was associated with the formation of integrin focal adhesion complexes around apoptotic eosinophils.Conclusions: These data further suggest a nonpassive role for airway epithelium in the resolution of eosinophilic inflammation in asthma.

AB - Background: We previously demonstrated receptor-mediated apoptotic-eosinophil engulfment by human small airway epithelial cells, which may represent a potentially important mechanism in the resolution of allergic and asthmatic inflammation.Objective: A549 cells were selected as being representative of alveolar epithelial cells, and their ability to ingest human apoptotic eosinophils was examined in terms of the effects of dexamethasone treatment and the receptor-mediated recognition mechanisms important in this process.Methods: A549 epithelial-cell expression of alphav beta3, alphav beta5, CD36, and the phosphatidylserine receptor was established by using immunostaining and flow cytometry, and inhibition assays were examined by using the role of these receptors in apoptotic-eosinophil recognition by resting and dexamethasone-treated A549 epithelial cells. Electron microscopy confirmed engulfment of apoptotic eosinophils, and receptor clustering around apoptotic eosinophils was examined by using immunofluorescent labeling.Results: A549 epithelial cells recognized and engulfed apoptotic eosinophils but not freshly isolated cells. Dexamethasone enhanced the number of A549 cells ingesting apoptotic eosinophils and dose dependently increased their capacity for ingestion. The tetrapeptide RGDS significantly inhibited apoptotic-eosinophil uptake by A549 cells, indicating a role for integrins in the recognition process. A549 cells expressed alphav beta3, alphav beta5, beta5, CD36, and the phosphatidylserine receptor, and expression of these receptors was not significantly increased after dexamethasone treatment. Engulfment of apoptotic eosinophils by A549 cells involved alphav beta3-, CD36-, alphav beta5-, and phosphatidylserine receptor-mediated recognition mechanisms and was associated with the formation of integrin focal adhesion complexes around apoptotic eosinophils.Conclusions: These data further suggest a nonpassive role for airway epithelium in the resolution of eosinophilic inflammation in asthma.

KW - epithelial cells

KW - eosinophils

KW - apoptosis

KW - asthma

KW - phagocytic recognition

KW - corticosteroids

KW - inflammatory cells

KW - dendritic cells

KW - T-lymphocytes

KW - in-vitro

KW - phagocytosis

KW - recognition

KW - alpha(V)beta(5)

KW - granulocytes

U2 - 10.1067/mai.2001.119414

DO - 10.1067/mai.2001.119414

M3 - Article

VL - 108

SP - 962

EP - 969

JO - Journal of Allergy and Clinical Immunology

JF - Journal of Allergy and Clinical Immunology

SN - 0091-6749

IS - 6

ER -