Human colonic microbiota associated with diet, obesity and weight loss

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620 Citations (Scopus)

Abstract

Background: It has been proposed that the development of obesity in humans is influenced by the relative proportions of the two major phyla of bacteria (Bacteroidetes and Firmicutes) present in the large intestine.
Objective: To examine the relationships between body mass index, weight loss and the major bacterial groups detected in fecal samples.
Design: Major groups of fecal bacteria were monitored using fluorescent in situ hybridization (FISH) in obese and non-obese subjects under conditions of weight maintenance, and in obese male volunteers undergoing weight loss on two different reduced carbohydrate weight-loss diets given successively for 4 weeks each.
Results: We detected no difference between obese and non-obese individuals in the proportion of Bacteroidetes measured in fecal samples, and no significant change in the percentage of Bacteroidetes in feces from obese subjects on weight loss diets. Significant diet-dependent reductions in a group of butyrate-producing Firmicutes were, however, detected in fecal samples from obese subjects on weight loss diets.
Conclusions: Diets designed to achieve weight loss in obese subjects can significantly alter the species composition of the gut microbiota, but we find no evidence that the proportions of Bacteroidetes and Firmicutes among fecal bacteria have a function in human obesity.

Original languageEnglish
Pages (from-to)1720-1724
Number of pages5
JournalInternational Journal of Obesity
Volume32
Issue number11
Early online date9 Sep 2008
DOIs
Publication statusPublished - Nov 2008

Keywords

  • weight loss
  • dietary carbohydrate
  • gut bacteria
  • bacteroidetes
  • firmicutes
  • 16S ribosomal-RNA
  • in-situ hybridization
  • human gut
  • oligonucleotide probes
  • diversity
  • bacteria
  • feces
  • communities
  • bacteroides
  • microflora

Cite this

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title = "Human colonic microbiota associated with diet, obesity and weight loss",
abstract = "Background: It has been proposed that the development of obesity in humans is influenced by the relative proportions of the two major phyla of bacteria (Bacteroidetes and Firmicutes) present in the large intestine. Objective: To examine the relationships between body mass index, weight loss and the major bacterial groups detected in fecal samples. Design: Major groups of fecal bacteria were monitored using fluorescent in situ hybridization (FISH) in obese and non-obese subjects under conditions of weight maintenance, and in obese male volunteers undergoing weight loss on two different reduced carbohydrate weight-loss diets given successively for 4 weeks each. Results: We detected no difference between obese and non-obese individuals in the proportion of Bacteroidetes measured in fecal samples, and no significant change in the percentage of Bacteroidetes in feces from obese subjects on weight loss diets. Significant diet-dependent reductions in a group of butyrate-producing Firmicutes were, however, detected in fecal samples from obese subjects on weight loss diets. Conclusions: Diets designed to achieve weight loss in obese subjects can significantly alter the species composition of the gut microbiota, but we find no evidence that the proportions of Bacteroidetes and Firmicutes among fecal bacteria have a function in human obesity.",
keywords = "weight loss, dietary carbohydrate, gut bacteria, bacteroidetes, firmicutes, 16S ribosomal-RNA, in-situ hybridization, human gut, oligonucleotide probes, diversity, bacteria, feces, communities, bacteroides, microflora",
author = "Duncan, {Sylvia Helen} and Gerald Lobley and Grietje Holtrop and J. Ince and Alexandra Johnstone and Petra Louis and Flint, {Harry James}",
year = "2008",
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T1 - Human colonic microbiota associated with diet, obesity and weight loss

AU - Duncan, Sylvia Helen

AU - Lobley, Gerald

AU - Holtrop, Grietje

AU - Ince, J.

AU - Johnstone, Alexandra

AU - Louis, Petra

AU - Flint, Harry James

PY - 2008/11

Y1 - 2008/11

N2 - Background: It has been proposed that the development of obesity in humans is influenced by the relative proportions of the two major phyla of bacteria (Bacteroidetes and Firmicutes) present in the large intestine. Objective: To examine the relationships between body mass index, weight loss and the major bacterial groups detected in fecal samples. Design: Major groups of fecal bacteria were monitored using fluorescent in situ hybridization (FISH) in obese and non-obese subjects under conditions of weight maintenance, and in obese male volunteers undergoing weight loss on two different reduced carbohydrate weight-loss diets given successively for 4 weeks each. Results: We detected no difference between obese and non-obese individuals in the proportion of Bacteroidetes measured in fecal samples, and no significant change in the percentage of Bacteroidetes in feces from obese subjects on weight loss diets. Significant diet-dependent reductions in a group of butyrate-producing Firmicutes were, however, detected in fecal samples from obese subjects on weight loss diets. Conclusions: Diets designed to achieve weight loss in obese subjects can significantly alter the species composition of the gut microbiota, but we find no evidence that the proportions of Bacteroidetes and Firmicutes among fecal bacteria have a function in human obesity.

AB - Background: It has been proposed that the development of obesity in humans is influenced by the relative proportions of the two major phyla of bacteria (Bacteroidetes and Firmicutes) present in the large intestine. Objective: To examine the relationships between body mass index, weight loss and the major bacterial groups detected in fecal samples. Design: Major groups of fecal bacteria were monitored using fluorescent in situ hybridization (FISH) in obese and non-obese subjects under conditions of weight maintenance, and in obese male volunteers undergoing weight loss on two different reduced carbohydrate weight-loss diets given successively for 4 weeks each. Results: We detected no difference between obese and non-obese individuals in the proportion of Bacteroidetes measured in fecal samples, and no significant change in the percentage of Bacteroidetes in feces from obese subjects on weight loss diets. Significant diet-dependent reductions in a group of butyrate-producing Firmicutes were, however, detected in fecal samples from obese subjects on weight loss diets. Conclusions: Diets designed to achieve weight loss in obese subjects can significantly alter the species composition of the gut microbiota, but we find no evidence that the proportions of Bacteroidetes and Firmicutes among fecal bacteria have a function in human obesity.

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KW - in-situ hybridization

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KW - oligonucleotide probes

KW - diversity

KW - bacteria

KW - feces

KW - communities

KW - bacteroides

KW - microflora

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