Human fetal testis: Second trimester proliferative and steroidogenic capacities

T J Murray, P A Fowler, D R Abramovich, N Haites, R G Lea

Research output: Contribution to journalArticle

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Abstract

The period of Leydig cell hyperplasia (14-18 weeks gestation) in human fetal testis is crucial for normal gonad development. We have studied the spatio-temporal distribution of key developmental and functional markers in human fetal testis between 13-19 weeks gestation. Proliferating cell nuclear antigen-positive cells were immunolocalized to both interstitium and tubules. Image analysis confirmed an increase in positive interstitial cells during Leydig cell hyperplasia (P < 0.05). c-Myc was localized to the interstitium with no gestational changes. The steroidogenic enzymes 3beta-hydroxysteroid dehydrogenase (protein) and cytochrome P450 17 alpha -hydroxylase/C17-20-lyase (P450c17; messenger ribonucleic acid and protein! were confined to the Leydig cells. The number of immunopositive cells increased between 13 and 19 weeks (P < 0.001). P450c17 mRNA (in situ hybridization) and protein were localized to the same population of interstitial Leydig cells. Androgen receptor and Bcl-2 protein (antiapoptotic) were gradually restricted to the peritubular myoid cells as gestation progressed. Conversely, Bax protein (pro-apoptotic) was predominantly localized to the tubule Sertoli cells, whereas the germ cells were Bax immunonegative.

In conclusion, human fetal Leydig cell hyperplasia is characterized by increasing numbers of proliferating cells and increased expression of steroidogenic enzymes. The Bcl-2-positive, Bax-negative status of the peritubular myoid cells may be a strategy for cell survival.

Original languageEnglish
Pages (from-to)4812-4817
Number of pages6
JournalJournal of Clinical Endocrinology and Metabolism
Volume85
Publication statusPublished - 2000

Keywords

  • PROGRAMMED CELL-DEATH
  • SEMEN QUALITY
  • RAT TESTIS
  • ANDROGEN RECEPTOR
  • SERTOLI CELLS
  • SPERM COUNTS
  • MEN
  • EXPRESSION
  • APOPTOSIS
  • ESTROGENS

Cite this

Human fetal testis: Second trimester proliferative and steroidogenic capacities. / Murray, T J ; Fowler, P A ; Abramovich, D R ; Haites, N ; Lea, R G .

In: Journal of Clinical Endocrinology and Metabolism, Vol. 85, 2000, p. 4812-4817.

Research output: Contribution to journalArticle

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N2 - The period of Leydig cell hyperplasia (14-18 weeks gestation) in human fetal testis is crucial for normal gonad development. We have studied the spatio-temporal distribution of key developmental and functional markers in human fetal testis between 13-19 weeks gestation. Proliferating cell nuclear antigen-positive cells were immunolocalized to both interstitium and tubules. Image analysis confirmed an increase in positive interstitial cells during Leydig cell hyperplasia (P < 0.05). c-Myc was localized to the interstitium with no gestational changes. The steroidogenic enzymes 3beta-hydroxysteroid dehydrogenase (protein) and cytochrome P450 17 alpha -hydroxylase/C17-20-lyase (P450c17; messenger ribonucleic acid and protein! were confined to the Leydig cells. The number of immunopositive cells increased between 13 and 19 weeks (P < 0.001). P450c17 mRNA (in situ hybridization) and protein were localized to the same population of interstitial Leydig cells. Androgen receptor and Bcl-2 protein (antiapoptotic) were gradually restricted to the peritubular myoid cells as gestation progressed. Conversely, Bax protein (pro-apoptotic) was predominantly localized to the tubule Sertoli cells, whereas the germ cells were Bax immunonegative.In conclusion, human fetal Leydig cell hyperplasia is characterized by increasing numbers of proliferating cells and increased expression of steroidogenic enzymes. The Bcl-2-positive, Bax-negative status of the peritubular myoid cells may be a strategy for cell survival.

AB - The period of Leydig cell hyperplasia (14-18 weeks gestation) in human fetal testis is crucial for normal gonad development. We have studied the spatio-temporal distribution of key developmental and functional markers in human fetal testis between 13-19 weeks gestation. Proliferating cell nuclear antigen-positive cells were immunolocalized to both interstitium and tubules. Image analysis confirmed an increase in positive interstitial cells during Leydig cell hyperplasia (P < 0.05). c-Myc was localized to the interstitium with no gestational changes. The steroidogenic enzymes 3beta-hydroxysteroid dehydrogenase (protein) and cytochrome P450 17 alpha -hydroxylase/C17-20-lyase (P450c17; messenger ribonucleic acid and protein! were confined to the Leydig cells. The number of immunopositive cells increased between 13 and 19 weeks (P < 0.001). P450c17 mRNA (in situ hybridization) and protein were localized to the same population of interstitial Leydig cells. Androgen receptor and Bcl-2 protein (antiapoptotic) were gradually restricted to the peritubular myoid cells as gestation progressed. Conversely, Bax protein (pro-apoptotic) was predominantly localized to the tubule Sertoli cells, whereas the germ cells were Bax immunonegative.In conclusion, human fetal Leydig cell hyperplasia is characterized by increasing numbers of proliferating cells and increased expression of steroidogenic enzymes. The Bcl-2-positive, Bax-negative status of the peritubular myoid cells may be a strategy for cell survival.

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KW - RAT TESTIS

KW - ANDROGEN RECEPTOR

KW - SERTOLI CELLS

KW - SPERM COUNTS

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KW - EXPRESSION

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JO - Journal of Clinical Endocrinology and Metabolism

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