Human galanin (GAL) and galanin 1 receptor (GALR1) variations are not involved in fat intake and early onset obesity

Nadine Schäuble, Kathrin Reichwald, Wolfgang Grassl, Helen Bechstein, Hans-Christian Müller, André Scherag, Frank Geller, Michael Utting, Wolfgang Siegfried, Hanspeter Goldschmidt, John Blundell, Clare Lawton, Rahul Alam, Stephen Whybrow, James Stubbs, Matthias Platzer, Johannes Hebebrand, Anke Hinney

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18 Citations (Scopus)

Abstract

The neuropeptide galanin (GAL) is involved in food intake and in fat ingestion. Presumably, these effects are conveyed via the galanin 1 receptor (GALR1). We screened the coding region of GAL (including 444 bp of its promoter region) and GALR1 for mutations using single-strand conformation polymorphism analysis and denaturing HPLC in up to 191 obese children and adolescents and 106 healthy underweight young adults (students). In GAL, we identified 3 novel single nucleotide polymorphisms (SNPs; silent: g.-419T-->C, g.-244G-->A; missense: g.47C-->T: Ala16Val) and one infrequent missense variation (c.253A-->G: Asn85Asp), and in GALR1 2 novel SNPs (silent: c.150C-->T, missense: c.793A-->T: Ile265Phe). To test for an association with obesity, we genotyped 7 SNPs (GAL: g.-244G-->A, g.47C-->T, rs7101947, rs1042577, rs3136540; GALR1: c.150C-->T, c.793A-->T) in up to 322 obese children and adolescents compared with up to 277 healthy underweight and normal weight young adults. Furthermore, we analyzed these SNPs with respect to potential effects on the percentage of energy consumed as fat in obese children and adolescents. Allele and genotype frequencies did not differ among the groups tested. In addition, we performed a pedigree transmission disequilibrium test (PDT) for one SNP (GAL: g.-244G-->A) in 610 (518 independent) obesity-trios (obese child or adolescent and both of its parents). However, the PDT for SNP GAL g.-244G-->A revealed no transmission disequilibrium. We conclude that the analyzed SNPs in GAL and GALR1 do not play a major role in early onset obesity or dietary fat intake in the obese children and adolescents of our study groups.
Original languageEnglish
Pages (from-to)1387-92
Number of pages6
JournalThe Journal of Nutrition
Volume135
Issue number6
Publication statusPublished - Jun 2005

Keywords

  • Adolescent
  • Adult
  • Case-Control Studies
  • Child
  • Dietary Fats
  • Eating
  • Female
  • Galanin
  • Genetic Variation
  • Humans
  • Male
  • Obesity
  • Polymorphism, Single Nucleotide
  • Receptor, Galanin, Type 1

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    Schäuble, N., Reichwald, K., Grassl, W., Bechstein, H., Müller, H-C., Scherag, A., Geller, F., Utting, M., Siegfried, W., Goldschmidt, H., Blundell, J., Lawton, C., Alam, R., Whybrow, S., Stubbs, J., Platzer, M., Hebebrand, J., & Hinney, A. (2005). Human galanin (GAL) and galanin 1 receptor (GALR1) variations are not involved in fat intake and early onset obesity. The Journal of Nutrition, 135(6), 1387-92.