Human galanin (GAL) and galanin 1 receptor (GALR1) variations are not involved in fat intake and early onset obesity

Nadine Schäuble, Kathrin Reichwald, Wolfgang Grassl, Helen Bechstein, Hans-Christian Müller, André Scherag, Frank Geller, Michael Utting, Wolfgang Siegfried, Hanspeter Goldschmidt, John Blundell, Clare Lawton, Rahul Alam, Stephen Whybrow, James Stubbs, Matthias Platzer, Johannes Hebebrand, Anke Hinney

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The neuropeptide galanin (GAL) is involved in food intake and in fat ingestion. Presumably, these effects are conveyed via the galanin 1 receptor (GALR1). We screened the coding region of GAL (including 444 bp of its promoter region) and GALR1 for mutations using single-strand conformation polymorphism analysis and denaturing HPLC in up to 191 obese children and adolescents and 106 healthy underweight young adults (students). In GAL, we identified 3 novel single nucleotide polymorphisms (SNPs; silent: g.-419T-->C, g.-244G-->A; missense: g.47C-->T: Ala16Val) and one infrequent missense variation (c.253A-->G: Asn85Asp), and in GALR1 2 novel SNPs (silent: c.150C-->T, missense: c.793A-->T: Ile265Phe). To test for an association with obesity, we genotyped 7 SNPs (GAL: g.-244G-->A, g.47C-->T, rs7101947, rs1042577, rs3136540; GALR1: c.150C-->T, c.793A-->T) in up to 322 obese children and adolescents compared with up to 277 healthy underweight and normal weight young adults. Furthermore, we analyzed these SNPs with respect to potential effects on the percentage of energy consumed as fat in obese children and adolescents. Allele and genotype frequencies did not differ among the groups tested. In addition, we performed a pedigree transmission disequilibrium test (PDT) for one SNP (GAL: g.-244G-->A) in 610 (518 independent) obesity-trios (obese child or adolescent and both of its parents). However, the PDT for SNP GAL g.-244G-->A revealed no transmission disequilibrium. We conclude that the analyzed SNPs in GAL and GALR1 do not play a major role in early onset obesity or dietary fat intake in the obese children and adolescents of our study groups.
Original languageEnglish
Pages (from-to)1387-92
Number of pages6
JournalThe Journal of Nutrition
Volume135
Issue number6
Publication statusPublished - Jun 2005

Fingerprint

Receptor, Galanin, Type 1
Galanin
Single Nucleotide Polymorphism
Obesity
Fats
Thinness
Pedigree
Young Adult
Eating
Dietary Fats
Neuropeptides
Genetic Promoter Regions
Gene Frequency
Parents
Genotype
High Pressure Liquid Chromatography

Keywords

  • Adolescent
  • Adult
  • Case-Control Studies
  • Child
  • Dietary Fats
  • Eating
  • Female
  • Galanin
  • Genetic Variation
  • Humans
  • Male
  • Obesity
  • Polymorphism, Single Nucleotide
  • Receptor, Galanin, Type 1

Cite this

Schäuble, N., Reichwald, K., Grassl, W., Bechstein, H., Müller, H-C., Scherag, A., ... Hinney, A. (2005). Human galanin (GAL) and galanin 1 receptor (GALR1) variations are not involved in fat intake and early onset obesity. The Journal of Nutrition, 135(6), 1387-92.

Human galanin (GAL) and galanin 1 receptor (GALR1) variations are not involved in fat intake and early onset obesity. / Schäuble, Nadine; Reichwald, Kathrin; Grassl, Wolfgang; Bechstein, Helen; Müller, Hans-Christian; Scherag, André; Geller, Frank; Utting, Michael; Siegfried, Wolfgang; Goldschmidt, Hanspeter; Blundell, John; Lawton, Clare; Alam, Rahul; Whybrow, Stephen; Stubbs, James; Platzer, Matthias; Hebebrand, Johannes; Hinney, Anke.

In: The Journal of Nutrition, Vol. 135, No. 6, 06.2005, p. 1387-92.

Research output: Contribution to journalArticle

Schäuble, N, Reichwald, K, Grassl, W, Bechstein, H, Müller, H-C, Scherag, A, Geller, F, Utting, M, Siegfried, W, Goldschmidt, H, Blundell, J, Lawton, C, Alam, R, Whybrow, S, Stubbs, J, Platzer, M, Hebebrand, J & Hinney, A 2005, 'Human galanin (GAL) and galanin 1 receptor (GALR1) variations are not involved in fat intake and early onset obesity', The Journal of Nutrition, vol. 135, no. 6, pp. 1387-92.
Schäuble N, Reichwald K, Grassl W, Bechstein H, Müller H-C, Scherag A et al. Human galanin (GAL) and galanin 1 receptor (GALR1) variations are not involved in fat intake and early onset obesity. The Journal of Nutrition. 2005 Jun;135(6):1387-92.
Schäuble, Nadine ; Reichwald, Kathrin ; Grassl, Wolfgang ; Bechstein, Helen ; Müller, Hans-Christian ; Scherag, André ; Geller, Frank ; Utting, Michael ; Siegfried, Wolfgang ; Goldschmidt, Hanspeter ; Blundell, John ; Lawton, Clare ; Alam, Rahul ; Whybrow, Stephen ; Stubbs, James ; Platzer, Matthias ; Hebebrand, Johannes ; Hinney, Anke. / Human galanin (GAL) and galanin 1 receptor (GALR1) variations are not involved in fat intake and early onset obesity. In: The Journal of Nutrition. 2005 ; Vol. 135, No. 6. pp. 1387-92.
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T1 - Human galanin (GAL) and galanin 1 receptor (GALR1) variations are not involved in fat intake and early onset obesity

AU - Schäuble, Nadine

AU - Reichwald, Kathrin

AU - Grassl, Wolfgang

AU - Bechstein, Helen

AU - Müller, Hans-Christian

AU - Scherag, André

AU - Geller, Frank

AU - Utting, Michael

AU - Siegfried, Wolfgang

AU - Goldschmidt, Hanspeter

AU - Blundell, John

AU - Lawton, Clare

AU - Alam, Rahul

AU - Whybrow, Stephen

AU - Stubbs, James

AU - Platzer, Matthias

AU - Hebebrand, Johannes

AU - Hinney, Anke

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N2 - The neuropeptide galanin (GAL) is involved in food intake and in fat ingestion. Presumably, these effects are conveyed via the galanin 1 receptor (GALR1). We screened the coding region of GAL (including 444 bp of its promoter region) and GALR1 for mutations using single-strand conformation polymorphism analysis and denaturing HPLC in up to 191 obese children and adolescents and 106 healthy underweight young adults (students). In GAL, we identified 3 novel single nucleotide polymorphisms (SNPs; silent: g.-419T-->C, g.-244G-->A; missense: g.47C-->T: Ala16Val) and one infrequent missense variation (c.253A-->G: Asn85Asp), and in GALR1 2 novel SNPs (silent: c.150C-->T, missense: c.793A-->T: Ile265Phe). To test for an association with obesity, we genotyped 7 SNPs (GAL: g.-244G-->A, g.47C-->T, rs7101947, rs1042577, rs3136540; GALR1: c.150C-->T, c.793A-->T) in up to 322 obese children and adolescents compared with up to 277 healthy underweight and normal weight young adults. Furthermore, we analyzed these SNPs with respect to potential effects on the percentage of energy consumed as fat in obese children and adolescents. Allele and genotype frequencies did not differ among the groups tested. In addition, we performed a pedigree transmission disequilibrium test (PDT) for one SNP (GAL: g.-244G-->A) in 610 (518 independent) obesity-trios (obese child or adolescent and both of its parents). However, the PDT for SNP GAL g.-244G-->A revealed no transmission disequilibrium. We conclude that the analyzed SNPs in GAL and GALR1 do not play a major role in early onset obesity or dietary fat intake in the obese children and adolescents of our study groups.

AB - The neuropeptide galanin (GAL) is involved in food intake and in fat ingestion. Presumably, these effects are conveyed via the galanin 1 receptor (GALR1). We screened the coding region of GAL (including 444 bp of its promoter region) and GALR1 for mutations using single-strand conformation polymorphism analysis and denaturing HPLC in up to 191 obese children and adolescents and 106 healthy underweight young adults (students). In GAL, we identified 3 novel single nucleotide polymorphisms (SNPs; silent: g.-419T-->C, g.-244G-->A; missense: g.47C-->T: Ala16Val) and one infrequent missense variation (c.253A-->G: Asn85Asp), and in GALR1 2 novel SNPs (silent: c.150C-->T, missense: c.793A-->T: Ile265Phe). To test for an association with obesity, we genotyped 7 SNPs (GAL: g.-244G-->A, g.47C-->T, rs7101947, rs1042577, rs3136540; GALR1: c.150C-->T, c.793A-->T) in up to 322 obese children and adolescents compared with up to 277 healthy underweight and normal weight young adults. Furthermore, we analyzed these SNPs with respect to potential effects on the percentage of energy consumed as fat in obese children and adolescents. Allele and genotype frequencies did not differ among the groups tested. In addition, we performed a pedigree transmission disequilibrium test (PDT) for one SNP (GAL: g.-244G-->A) in 610 (518 independent) obesity-trios (obese child or adolescent and both of its parents). However, the PDT for SNP GAL g.-244G-->A revealed no transmission disequilibrium. We conclude that the analyzed SNPs in GAL and GALR1 do not play a major role in early onset obesity or dietary fat intake in the obese children and adolescents of our study groups.

KW - Adolescent

KW - Adult

KW - Case-Control Studies

KW - Child

KW - Dietary Fats

KW - Eating

KW - Female

KW - Galanin

KW - Genetic Variation

KW - Humans

KW - Male

KW - Obesity

KW - Polymorphism, Single Nucleotide

KW - Receptor, Galanin, Type 1

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VL - 135

SP - 1387

EP - 1392

JO - The Journal of Nutrition

JF - The Journal of Nutrition

SN - 0022-3166

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ER -