Human platelet alloantigens (HPAs)

PCR-SSP genotyping of a UK population for 15 HPA alleles

D C Jones, M Bunce, S V Fuggle, Neil Thomas Young, S E Marshall

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Alloimmunization to human platelet alloantigens (HPAs) is responsible for neonatal alloimmune thrombocytopenia (NAIT), post-transfusional purpura (PTP) and platelet transfusion refractoriness. HPAs may also have a role as histocompatibility antigens in transplantation as well as associations with cardiac disease. We have developed a polymerase chain reaction-sequence-specific primer (PCR-SSP) assay capable of detecting 15 HPA allelic variants. As part of the validation of the assay, 134 UK renal donors were genotyped to determine HPA allele frequencies in the UK population. The HPA allele frequencies obtained are consistent with those of the other European studies: GP1A*1 (HPA-5a) and GP1A*2 (HPA-5b), 0.914 and 0.086, respectively; GP1BA*1 (HPA-2a) and GP1BA*2 (HPA-2b), 0.925 and 0.075; GP2B*1 (HPA-3a) and GP2B*2 (HPA-3b), 0.627 and 0.373; GP3A*1 (HPA-1a) and GP3A*2 (HPA-1b), 0.840 and 0.161. The rare alleles GP2B*3 (HPA-9bw) and GP3A*3 to *8 (HPA-4b, -6b, -7bw, -8bw, -10bw and -11bw, respectively) were all absent. This comprehensive HPA genotyping assay allows rapid, accurate and reproducible results at low cost.
Original languageEnglish
Pages (from-to)415-419
Number of pages5
JournalEuropean Journal of Immunogenetics
Volume30
Issue number6
DOIs
Publication statusPublished - 1 Dec 2003

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Human Platelet Antigens
Alleles
Polymerase Chain Reaction
Population
Gene Frequency
Neonatal Alloimmune Thrombocytopenia
Platelet Transfusion
Histocompatibility Antigens
Purpura

Keywords

  • Alleles
  • Antigens, Human Platelet
  • Gene Frequency
  • Genetics, Population
  • Great Britain
  • Humans
  • Polymerase Chain Reaction
  • Reproducibility of Results

Cite this

Human platelet alloantigens (HPAs) : PCR-SSP genotyping of a UK population for 15 HPA alleles. / Jones, D C; Bunce, M; Fuggle, S V; Young, Neil Thomas; Marshall, S E.

In: European Journal of Immunogenetics, Vol. 30, No. 6, 01.12.2003, p. 415-419.

Research output: Contribution to journalArticle

Jones, D C ; Bunce, M ; Fuggle, S V ; Young, Neil Thomas ; Marshall, S E. / Human platelet alloantigens (HPAs) : PCR-SSP genotyping of a UK population for 15 HPA alleles. In: European Journal of Immunogenetics. 2003 ; Vol. 30, No. 6. pp. 415-419.
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abstract = "Alloimmunization to human platelet alloantigens (HPAs) is responsible for neonatal alloimmune thrombocytopenia (NAIT), post-transfusional purpura (PTP) and platelet transfusion refractoriness. HPAs may also have a role as histocompatibility antigens in transplantation as well as associations with cardiac disease. We have developed a polymerase chain reaction-sequence-specific primer (PCR-SSP) assay capable of detecting 15 HPA allelic variants. As part of the validation of the assay, 134 UK renal donors were genotyped to determine HPA allele frequencies in the UK population. The HPA allele frequencies obtained are consistent with those of the other European studies: GP1A*1 (HPA-5a) and GP1A*2 (HPA-5b), 0.914 and 0.086, respectively; GP1BA*1 (HPA-2a) and GP1BA*2 (HPA-2b), 0.925 and 0.075; GP2B*1 (HPA-3a) and GP2B*2 (HPA-3b), 0.627 and 0.373; GP3A*1 (HPA-1a) and GP3A*2 (HPA-1b), 0.840 and 0.161. The rare alleles GP2B*3 (HPA-9bw) and GP3A*3 to *8 (HPA-4b, -6b, -7bw, -8bw, -10bw and -11bw, respectively) were all absent. This comprehensive HPA genotyping assay allows rapid, accurate and reproducible results at low cost.",
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KW - Alleles

KW - Antigens, Human Platelet

KW - Gene Frequency

KW - Genetics, Population

KW - Great Britain

KW - Humans

KW - Polymerase Chain Reaction

KW - Reproducibility of Results

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