Abstract
AIM: The aim of the study was to assess the function of human skeletal muscle myosin across a wide range of temperatures, including physiological. METHODS: We used a single fibre in vitro motility assay. The in vitro motility speed of actin filaments propelled by myosin extracted from fibres expressing type I myosin heavy chain (MyHC; n = 9), IIa MyHC (n = 6), IIax MyHC (n = 4) and I/IIa MyHC (n = 1) was measured at 15, 20, 25, 30 and 35 degrees C. RESULTS: The motility speed between groups of fibres expressing different MyHC differed significantly (P
Original language | English |
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Pages (from-to) | 151-158 |
Number of pages | 8 |
Journal | Acta Physiologica |
Volume | 186 |
Issue number | 2 |
Early online date | 22 Dec 2005 |
DOIs | |
Publication status | Published - 1 Feb 2006 |
Keywords
- Adult
- Animals
- Electrophoresis, Polyacrylamide Gel
- Humans
- Male
- Microfilaments
- Muscle Contraction
- Muscle Fibers, Skeletal
- Muscle, Skeletal
- Protein Isoforms
- Rabbits
- Skeletal Muscle Myosins
- Temperature