Humoral immune response of the small-spotted catshark, Scyliorhinus canicula

Kathryn Crouch, Lauren E Smith, Rebecca Williams, Wei Cao, Mike Lee, Allan Jensen, Helen Dooley

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Cartilaginous fishes are the oldest group in which an adaptive immune system based on immunoglobulin-superfamily members is found. This manuscript compares humoral immune function in small-spotted catshark (Scyliorhinus canicula) with that described for spiny dogfish (Squalus acanthias), another member of the Squalomorphi superorder, and nurse shark, the model for humoral immunity in elasmobranchs and a member of the Galeomorphi superorder. Although small-spotted catshark and nurse shark are separated by over 200 million years we found that immunoglobulin isoforms are well conserved between the two species. However, the plasma protein profile of small-spotted catshark was most similar to that of spiny dogfish, with low levels of pentameric IgM, and IgNAR present as a multimer in plasma rather than a monomer. We show that an antigen-specific monomeric IgM response, with a profile similar to that described previously for nurse sharks, can be raised in small-spotted catshark. Lacking polyclonal or monoclonal antibody reagents for detecting catshark IgNAR we investigated phage-display and recombinant Fc-fusion protein expression as alternative methods to look for an antigen-specific response for this isotype. However, we could find no evidence of an antigen-specific IgNAR in the animals tested using either of these techniques. Thus, unlike nurse sharks where antigen-specific monomeric IgM and IgNAR appear together, it seems there may be a temporal or complete 'uncoupling' of these isotypes during a humoral response in the small-spotted catshark.
Original languageEnglish
Pages (from-to)1158-1169
Number of pages12
JournalFish & Shellfish Immunology
Volume34
Issue number5
Early online date23 Feb 2013
DOIs
Publication statusPublished - May 2013

Fingerprint

Scyliorhinus canicula
shark
Ginglymostoma cirratum
antigen
immune response
humoral immunity
Squalus acanthias
Immunoglobulin M
Antigens
antigens
Immunoglobulins
plasma
immunoglobulins
Bacteriophages
protein
Immune system
immune system
immunity
recombinant fusion proteins
Fish

Keywords

  • cartilaginous fish
  • shark
  • adaptive immunity
  • immunoglobin
  • IgM
  • IgNAR
  • phage-display

Cite this

Crouch, K., Smith, L. E., Williams, R., Cao, W., Lee, M., Jensen, A., & Dooley, H. (2013). Humoral immune response of the small-spotted catshark, Scyliorhinus canicula. Fish & Shellfish Immunology, 34(5), 1158-1169. https://doi.org/10.1016/j.fsi.2013.01.025

Humoral immune response of the small-spotted catshark, Scyliorhinus canicula. / Crouch, Kathryn; Smith, Lauren E; Williams, Rebecca; Cao, Wei; Lee, Mike; Jensen, Allan; Dooley, Helen.

In: Fish & Shellfish Immunology, Vol. 34, No. 5, 05.2013, p. 1158-1169.

Research output: Contribution to journalArticle

Crouch, K, Smith, LE, Williams, R, Cao, W, Lee, M, Jensen, A & Dooley, H 2013, 'Humoral immune response of the small-spotted catshark, Scyliorhinus canicula', Fish & Shellfish Immunology, vol. 34, no. 5, pp. 1158-1169. https://doi.org/10.1016/j.fsi.2013.01.025
Crouch, Kathryn ; Smith, Lauren E ; Williams, Rebecca ; Cao, Wei ; Lee, Mike ; Jensen, Allan ; Dooley, Helen. / Humoral immune response of the small-spotted catshark, Scyliorhinus canicula. In: Fish & Shellfish Immunology. 2013 ; Vol. 34, No. 5. pp. 1158-1169.
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