Hyperpnea-induced bronchoconstriction and urinary CC16 levels in athletes

Claire Bolger, Ellen Tufvesson, Malcolm Sue-Chu, Graham Devereux, Jon G. Ayres, Leif Bjermer, Pascale Kippelen

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Purpose: Exercise-induced bronchoconstriction (EIB) is a common condition in both individuals with asthma and otherwise healthy elite athletes. Although excessive water loss by peripheral airways during hyperpnea is regarded as the initial trigger for EIB, the cascade of events that follows remains unclear. Our goal was to establish whether transient disruption of the airway epithelial barrier occurs after a short period of hyperpnea of dry air in athletes with EIB.

Methods: Urinary concentration of the pneumoprotein Clara cell (CC16) was used as an assumed biomarker of lung epithelial cell damage or dysfunction. Samples were collected at baseline and for 90 min after an 8-min eucapnic voluntary hyperpnea (EVH) test in 50 female individuals (28 athletes and 22 untrained).

Results: Nineteen subjects (10 athletes) demonstrated a sustained bronchoconstriction after EVH (mean ± SE forced expiratory volume in the first second (FEV1) fall from baseline = 23.4% ± 2.6%). The remaining subjects had a negative challenge result with an FEV1 fall of 5.9% ± 0.6%. An increase (P < 0.001) in urinary CC16 concentration was noticed after EVH in all but one subject, with no group difference (median CC16 increase before to after challenge: athletes EVH- 0.083 ng·µmol-1, athletes EVH+ 0.223 ng·µmol-1, untrained EVH- 0.074 ng·µmol-1, untrained EVH+ 0.571 ng·µmol-1; P > 0.05).

Conclusions: Urinary levels of CC16 are increased after EVH in all individuals (trained and untrained, with and without EIB) suggestive of dehydration-induced perturbation of the distal respiratory epithelium during episodes of hyperventilation.
Original languageEnglish
Pages (from-to)1207-1213
Number of pages7
JournalMedicine and Science in Sports and Exercise
Volume43
Issue number7
DOIs
Publication statusPublished - Jul 2011

Fingerprint

Bronchoconstriction
Athletes
Exercise
Respiratory Mucosa
Hyperventilation
Forced Expiratory Volume
Dehydration
Asthma
Biomarkers
Epithelial Cells
Air
Lung
Water

Keywords

  • respiratory function tests
  • young adult
  • humans
  • uteroglobin
  • asthma, exercise-induced
  • exercise test
  • adult
  • bronchoconstriction
  • hyperventilation
  • middle aged
  • adolescent
  • female
  • athletes

Cite this

Hyperpnea-induced bronchoconstriction and urinary CC16 levels in athletes. / Bolger, Claire; Tufvesson, Ellen; Sue-Chu, Malcolm; Devereux, Graham; Ayres, Jon G.; Bjermer, Leif; Kippelen, Pascale.

In: Medicine and Science in Sports and Exercise, Vol. 43, No. 7, 07.2011, p. 1207-1213.

Research output: Contribution to journalArticle

Bolger, C, Tufvesson, E, Sue-Chu, M, Devereux, G, Ayres, JG, Bjermer, L & Kippelen, P 2011, 'Hyperpnea-induced bronchoconstriction and urinary CC16 levels in athletes', Medicine and Science in Sports and Exercise, vol. 43, no. 7, pp. 1207-1213. https://doi.org/10.1249/MSS.0b013e31820750d8
Bolger, Claire ; Tufvesson, Ellen ; Sue-Chu, Malcolm ; Devereux, Graham ; Ayres, Jon G. ; Bjermer, Leif ; Kippelen, Pascale. / Hyperpnea-induced bronchoconstriction and urinary CC16 levels in athletes. In: Medicine and Science in Sports and Exercise. 2011 ; Vol. 43, No. 7. pp. 1207-1213.
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abstract = "Purpose: Exercise-induced bronchoconstriction (EIB) is a common condition in both individuals with asthma and otherwise healthy elite athletes. Although excessive water loss by peripheral airways during hyperpnea is regarded as the initial trigger for EIB, the cascade of events that follows remains unclear. Our goal was to establish whether transient disruption of the airway epithelial barrier occurs after a short period of hyperpnea of dry air in athletes with EIB. Methods: Urinary concentration of the pneumoprotein Clara cell (CC16) was used as an assumed biomarker of lung epithelial cell damage or dysfunction. Samples were collected at baseline and for 90 min after an 8-min eucapnic voluntary hyperpnea (EVH) test in 50 female individuals (28 athletes and 22 untrained). Results: Nineteen subjects (10 athletes) demonstrated a sustained bronchoconstriction after EVH (mean ± SE forced expiratory volume in the first second (FEV1) fall from baseline = 23.4{\%} ± 2.6{\%}). The remaining subjects had a negative challenge result with an FEV1 fall of 5.9{\%} ± 0.6{\%}. An increase (P < 0.001) in urinary CC16 concentration was noticed after EVH in all but one subject, with no group difference (median CC16 increase before to after challenge: athletes EVH- 0.083 ng·µmol-1, athletes EVH+ 0.223 ng·µmol-1, untrained EVH- 0.074 ng·µmol-1, untrained EVH+ 0.571 ng·µmol-1; P > 0.05). Conclusions: Urinary levels of CC16 are increased after EVH in all individuals (trained and untrained, with and without EIB) suggestive of dehydration-induced perturbation of the distal respiratory epithelium during episodes of hyperventilation.",
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AU - Tufvesson, Ellen

AU - Sue-Chu, Malcolm

AU - Devereux, Graham

AU - Ayres, Jon G.

AU - Bjermer, Leif

AU - Kippelen, Pascale

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N2 - Purpose: Exercise-induced bronchoconstriction (EIB) is a common condition in both individuals with asthma and otherwise healthy elite athletes. Although excessive water loss by peripheral airways during hyperpnea is regarded as the initial trigger for EIB, the cascade of events that follows remains unclear. Our goal was to establish whether transient disruption of the airway epithelial barrier occurs after a short period of hyperpnea of dry air in athletes with EIB. Methods: Urinary concentration of the pneumoprotein Clara cell (CC16) was used as an assumed biomarker of lung epithelial cell damage or dysfunction. Samples were collected at baseline and for 90 min after an 8-min eucapnic voluntary hyperpnea (EVH) test in 50 female individuals (28 athletes and 22 untrained). Results: Nineteen subjects (10 athletes) demonstrated a sustained bronchoconstriction after EVH (mean ± SE forced expiratory volume in the first second (FEV1) fall from baseline = 23.4% ± 2.6%). The remaining subjects had a negative challenge result with an FEV1 fall of 5.9% ± 0.6%. An increase (P < 0.001) in urinary CC16 concentration was noticed after EVH in all but one subject, with no group difference (median CC16 increase before to after challenge: athletes EVH- 0.083 ng·µmol-1, athletes EVH+ 0.223 ng·µmol-1, untrained EVH- 0.074 ng·µmol-1, untrained EVH+ 0.571 ng·µmol-1; P > 0.05). Conclusions: Urinary levels of CC16 are increased after EVH in all individuals (trained and untrained, with and without EIB) suggestive of dehydration-induced perturbation of the distal respiratory epithelium during episodes of hyperventilation.

AB - Purpose: Exercise-induced bronchoconstriction (EIB) is a common condition in both individuals with asthma and otherwise healthy elite athletes. Although excessive water loss by peripheral airways during hyperpnea is regarded as the initial trigger for EIB, the cascade of events that follows remains unclear. Our goal was to establish whether transient disruption of the airway epithelial barrier occurs after a short period of hyperpnea of dry air in athletes with EIB. Methods: Urinary concentration of the pneumoprotein Clara cell (CC16) was used as an assumed biomarker of lung epithelial cell damage or dysfunction. Samples were collected at baseline and for 90 min after an 8-min eucapnic voluntary hyperpnea (EVH) test in 50 female individuals (28 athletes and 22 untrained). Results: Nineteen subjects (10 athletes) demonstrated a sustained bronchoconstriction after EVH (mean ± SE forced expiratory volume in the first second (FEV1) fall from baseline = 23.4% ± 2.6%). The remaining subjects had a negative challenge result with an FEV1 fall of 5.9% ± 0.6%. An increase (P < 0.001) in urinary CC16 concentration was noticed after EVH in all but one subject, with no group difference (median CC16 increase before to after challenge: athletes EVH- 0.083 ng·µmol-1, athletes EVH+ 0.223 ng·µmol-1, untrained EVH- 0.074 ng·µmol-1, untrained EVH+ 0.571 ng·µmol-1; P > 0.05). Conclusions: Urinary levels of CC16 are increased after EVH in all individuals (trained and untrained, with and without EIB) suggestive of dehydration-induced perturbation of the distal respiratory epithelium during episodes of hyperventilation.

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KW - humans

KW - uteroglobin

KW - asthma, exercise-induced

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KW - bronchoconstriction

KW - hyperventilation

KW - middle aged

KW - adolescent

KW - female

KW - athletes

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