Hypothalamic gene expression in sheep for cocaine- and amphetamine-regulated transcript, pro-opiomelanocortin, neuropeptide Y, agouti-related peptide and leptin receptor and responses to negative energy balance

Clare Lesley Adam, Z A Archer, Patricia Findlay, L Thomas, M Marie

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

Hypothalamic pathways involved in the regulation of energy balance have not been widely studied in ruminants to date. Here, we used in situ hybridisation to study the gene expression of a number of leptin-sensitive receptors and neuropeptides in the ovine hypothalamus. Gene expression was first localised for cocaine- and amphetamine-regulated transcript (CART) and agouti-related peptide (AGRP). We then examined in adult male castrated sheep the effects of acute negative energy balance induced by a 4-day fast on the amounts of these mRNAs and those for leptin receptor (OB-Rb), neuropeptide Y (NPY) and pro-opiomelanocortin (POMC). CART mRNA was localised in the arcuate nucleus (ARC), para-ventricular nucleus, median eminence and ventromedial hypothalamic nucleus, and extensive co-localisation with POMC mRNA was demonstrated in the ARC. AGRP mRNA was localised in the ARC. Fasting up-regulated gene expression for OB-Rb and for the orexigenic neuropeptides NPY and AGRP in the ARC. There was a trend towards down-regulation of gene expression for the anorexigenic neuropeptide CART and no effect on POMC in the ARC, although these results are inconclusive. The presence or absence of oestradiol-containing subcutaneous implants did not influence gene expression or the effects of fasting. The hypothalamic changes were consistent with responses to the observed reduction in circulation leptin and suggest that the peripheral feedback and central mechanisms for restoring the energy balance may be largely conserved across monogastric and ruminant species.

Original languageEnglish
Pages (from-to)250-256
Number of pages7
JournalNeuroendocrinology
Volume75
Issue number4
DOIs
Publication statusPublished - Apr 2002

Keywords

  • neuropeptide Y
  • cocaine and amphetamine-regulated transcript proopiomelanocortin
  • agouti-related peptide
  • food restriction
  • leptin
  • orexins
  • gonadal steroids
  • arcuate nucleus
  • paraventricular nucleus
  • ventromedial nucleus
  • ovine
  • LUTEINIZING-HORMONE SECRETION
  • MESSENGER-RNA
  • MOUSE HYPOTHALAMUS
  • FOOD RESTRICTION
  • CART NEURONS
  • LONG FORM
  • BRAIN
  • PROOPIOMELANOCORTIN
  • PROTEIN
  • PHOTOPERIOD
  • Neuropeptide Y
  • Cocaine and amphetamine-regulated transcript
  • Proopiomelanocortin
  • Agouti-related peptide
  • Food restriction
  • Leptin
  • Orexins
  • Gonadal steroids
  • Arcuate nucleus
  • Paraventricular nucleus
  • Ventromedial nucleus
  • Ovine

Cite this

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title = "Hypothalamic gene expression in sheep for cocaine- and amphetamine-regulated transcript, pro-opiomelanocortin, neuropeptide Y, agouti-related peptide and leptin receptor and responses to negative energy balance",
abstract = "Hypothalamic pathways involved in the regulation of energy balance have not been widely studied in ruminants to date. Here, we used in situ hybridisation to study the gene expression of a number of leptin-sensitive receptors and neuropeptides in the ovine hypothalamus. Gene expression was first localised for cocaine- and amphetamine-regulated transcript (CART) and agouti-related peptide (AGRP). We then examined in adult male castrated sheep the effects of acute negative energy balance induced by a 4-day fast on the amounts of these mRNAs and those for leptin receptor (OB-Rb), neuropeptide Y (NPY) and pro-opiomelanocortin (POMC). CART mRNA was localised in the arcuate nucleus (ARC), para-ventricular nucleus, median eminence and ventromedial hypothalamic nucleus, and extensive co-localisation with POMC mRNA was demonstrated in the ARC. AGRP mRNA was localised in the ARC. Fasting up-regulated gene expression for OB-Rb and for the orexigenic neuropeptides NPY and AGRP in the ARC. There was a trend towards down-regulation of gene expression for the anorexigenic neuropeptide CART and no effect on POMC in the ARC, although these results are inconclusive. The presence or absence of oestradiol-containing subcutaneous implants did not influence gene expression or the effects of fasting. The hypothalamic changes were consistent with responses to the observed reduction in circulation leptin and suggest that the peripheral feedback and central mechanisms for restoring the energy balance may be largely conserved across monogastric and ruminant species.",
keywords = "neuropeptide Y, cocaine and amphetamine-regulated transcript proopiomelanocortin, agouti-related peptide, food restriction, leptin, orexins, gonadal steroids, arcuate nucleus, paraventricular nucleus, ventromedial nucleus, ovine, LUTEINIZING-HORMONE SECRETION, MESSENGER-RNA, MOUSE HYPOTHALAMUS, FOOD RESTRICTION, CART NEURONS, LONG FORM, BRAIN, PROOPIOMELANOCORTIN, PROTEIN, PHOTOPERIOD, Neuropeptide Y, Cocaine and amphetamine-regulated transcript, Proopiomelanocortin, Agouti-related peptide, Food restriction, Leptin, Orexins, Gonadal steroids, Arcuate nucleus, Paraventricular nucleus, Ventromedial nucleus, Ovine",
author = "Adam, {Clare Lesley} and Archer, {Z A} and Patricia Findlay and L Thomas and M Marie",
year = "2002",
month = "4",
doi = "10.1159/000054716",
language = "English",
volume = "75",
pages = "250--256",
journal = "Neuroendocrinology",
issn = "0028-3835",
publisher = "S. Karger AG",
number = "4",

}

TY - JOUR

T1 - Hypothalamic gene expression in sheep for cocaine- and amphetamine-regulated transcript, pro-opiomelanocortin, neuropeptide Y, agouti-related peptide and leptin receptor and responses to negative energy balance

AU - Adam, Clare Lesley

AU - Archer, Z A

AU - Findlay, Patricia

AU - Thomas, L

AU - Marie, M

PY - 2002/4

Y1 - 2002/4

N2 - Hypothalamic pathways involved in the regulation of energy balance have not been widely studied in ruminants to date. Here, we used in situ hybridisation to study the gene expression of a number of leptin-sensitive receptors and neuropeptides in the ovine hypothalamus. Gene expression was first localised for cocaine- and amphetamine-regulated transcript (CART) and agouti-related peptide (AGRP). We then examined in adult male castrated sheep the effects of acute negative energy balance induced by a 4-day fast on the amounts of these mRNAs and those for leptin receptor (OB-Rb), neuropeptide Y (NPY) and pro-opiomelanocortin (POMC). CART mRNA was localised in the arcuate nucleus (ARC), para-ventricular nucleus, median eminence and ventromedial hypothalamic nucleus, and extensive co-localisation with POMC mRNA was demonstrated in the ARC. AGRP mRNA was localised in the ARC. Fasting up-regulated gene expression for OB-Rb and for the orexigenic neuropeptides NPY and AGRP in the ARC. There was a trend towards down-regulation of gene expression for the anorexigenic neuropeptide CART and no effect on POMC in the ARC, although these results are inconclusive. The presence or absence of oestradiol-containing subcutaneous implants did not influence gene expression or the effects of fasting. The hypothalamic changes were consistent with responses to the observed reduction in circulation leptin and suggest that the peripheral feedback and central mechanisms for restoring the energy balance may be largely conserved across monogastric and ruminant species.

AB - Hypothalamic pathways involved in the regulation of energy balance have not been widely studied in ruminants to date. Here, we used in situ hybridisation to study the gene expression of a number of leptin-sensitive receptors and neuropeptides in the ovine hypothalamus. Gene expression was first localised for cocaine- and amphetamine-regulated transcript (CART) and agouti-related peptide (AGRP). We then examined in adult male castrated sheep the effects of acute negative energy balance induced by a 4-day fast on the amounts of these mRNAs and those for leptin receptor (OB-Rb), neuropeptide Y (NPY) and pro-opiomelanocortin (POMC). CART mRNA was localised in the arcuate nucleus (ARC), para-ventricular nucleus, median eminence and ventromedial hypothalamic nucleus, and extensive co-localisation with POMC mRNA was demonstrated in the ARC. AGRP mRNA was localised in the ARC. Fasting up-regulated gene expression for OB-Rb and for the orexigenic neuropeptides NPY and AGRP in the ARC. There was a trend towards down-regulation of gene expression for the anorexigenic neuropeptide CART and no effect on POMC in the ARC, although these results are inconclusive. The presence or absence of oestradiol-containing subcutaneous implants did not influence gene expression or the effects of fasting. The hypothalamic changes were consistent with responses to the observed reduction in circulation leptin and suggest that the peripheral feedback and central mechanisms for restoring the energy balance may be largely conserved across monogastric and ruminant species.

KW - neuropeptide Y

KW - cocaine and amphetamine-regulated transcript proopiomelanocortin

KW - agouti-related peptide

KW - food restriction

KW - leptin

KW - orexins

KW - gonadal steroids

KW - arcuate nucleus

KW - paraventricular nucleus

KW - ventromedial nucleus

KW - ovine

KW - LUTEINIZING-HORMONE SECRETION

KW - MESSENGER-RNA

KW - MOUSE HYPOTHALAMUS

KW - FOOD RESTRICTION

KW - CART NEURONS

KW - LONG FORM

KW - BRAIN

KW - PROOPIOMELANOCORTIN

KW - PROTEIN

KW - PHOTOPERIOD

KW - Neuropeptide Y

KW - Cocaine and amphetamine-regulated transcript

KW - Proopiomelanocortin

KW - Agouti-related peptide

KW - Food restriction

KW - Leptin

KW - Orexins

KW - Gonadal steroids

KW - Arcuate nucleus

KW - Paraventricular nucleus

KW - Ventromedial nucleus

KW - Ovine

U2 - 10.1159/000054716

DO - 10.1159/000054716

M3 - Article

VL - 75

SP - 250

EP - 256

JO - Neuroendocrinology

JF - Neuroendocrinology

SN - 0028-3835

IS - 4

ER -