Hypoxia stimulates 18F-Fluorodeoxyglucose uptake in breast cancer cells via hypoxia inducible factor-1 and AMP-activated protein kinase

Tim A. D. Smith, Matteo Zanda, Ian N. Fleming

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Introduction
Hypoxia can stimulate 18F-fluorodeoxyglucose (FDG) uptake in cultured cells. A better understanding of the underlying molecular mechanism is required to determine the value of FDG for studying tumour hypoxia.

Methods
The effect of hypoxia on FDG uptake, and key proteins involved in glucose transport and glycolysis, was studied in MCF7 and MDA231 breast cancer cell lines.

Results
Hypoxia induced a dose- and time-dependent increase in FDG uptake. The FDG increase was transient, suggesting that FDG uptake is only likely to be increased by acute hypoxia (< 24 h). Molecular analysis indicated that hypoxia upregulated glut1 and 6-phosphofructo-2-kinase, key proteins involved in regulating glucose transport and glycolysis, and that these changes were induced by Hypoxia-Inducible factor 1 (HIF1) upregulation and/or AMP-activated protein kinase activation.

Conclusions
FDG may provide useful information about the oxygenation status of cells in hypoxic regions where HIF1 upregulation is hypoxia-driven.
Original languageEnglish
Pages (from-to)858-864
Number of pages7
JournalNuclear Medicine and Biology
Volume40
Issue number6
DOIs
Publication statusPublished - Aug 2013

Fingerprint

Hypoxia-Inducible Factor 1
Cell Hypoxia
AMP-Activated Protein Kinases
Fluorodeoxyglucose F18
Breast Neoplasms
Glycolysis
Up-Regulation
Phosphofructokinase-2
Glucose
Cultured Cells
Proteins
Cell Line
Hypoxia

Keywords

  • AMP-activated protein kinase
  • fluorodeoxyglucose
  • hypoxia
  • hypoxia-inducible factor 1
  • oncology
  • positron emission tomography

Cite this

Hypoxia stimulates 18F-Fluorodeoxyglucose uptake in breast cancer cells via hypoxia inducible factor-1 and AMP-activated protein kinase. / Smith, Tim A. D.; Zanda, Matteo; Fleming, Ian N.

In: Nuclear Medicine and Biology, Vol. 40, No. 6, 08.2013, p. 858-864.

Research output: Contribution to journalArticle

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title = "Hypoxia stimulates 18F-Fluorodeoxyglucose uptake in breast cancer cells via hypoxia inducible factor-1 and AMP-activated protein kinase",
abstract = "IntroductionHypoxia can stimulate 18F-fluorodeoxyglucose (FDG) uptake in cultured cells. A better understanding of the underlying molecular mechanism is required to determine the value of FDG for studying tumour hypoxia.MethodsThe effect of hypoxia on FDG uptake, and key proteins involved in glucose transport and glycolysis, was studied in MCF7 and MDA231 breast cancer cell lines.ResultsHypoxia induced a dose- and time-dependent increase in FDG uptake. The FDG increase was transient, suggesting that FDG uptake is only likely to be increased by acute hypoxia (< 24 h). Molecular analysis indicated that hypoxia upregulated glut1 and 6-phosphofructo-2-kinase, key proteins involved in regulating glucose transport and glycolysis, and that these changes were induced by Hypoxia-Inducible factor 1 (HIF1) upregulation and/or AMP-activated protein kinase activation.ConclusionsFDG may provide useful information about the oxygenation status of cells in hypoxic regions where HIF1 upregulation is hypoxia-driven.",
keywords = "AMP-activated protein kinase, fluorodeoxyglucose, hypoxia, hypoxia-inducible factor 1, oncology, positron emission tomography",
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T1 - Hypoxia stimulates 18F-Fluorodeoxyglucose uptake in breast cancer cells via hypoxia inducible factor-1 and AMP-activated protein kinase

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AU - Zanda, Matteo

AU - Fleming, Ian N.

PY - 2013/8

Y1 - 2013/8

N2 - IntroductionHypoxia can stimulate 18F-fluorodeoxyglucose (FDG) uptake in cultured cells. A better understanding of the underlying molecular mechanism is required to determine the value of FDG for studying tumour hypoxia.MethodsThe effect of hypoxia on FDG uptake, and key proteins involved in glucose transport and glycolysis, was studied in MCF7 and MDA231 breast cancer cell lines.ResultsHypoxia induced a dose- and time-dependent increase in FDG uptake. The FDG increase was transient, suggesting that FDG uptake is only likely to be increased by acute hypoxia (< 24 h). Molecular analysis indicated that hypoxia upregulated glut1 and 6-phosphofructo-2-kinase, key proteins involved in regulating glucose transport and glycolysis, and that these changes were induced by Hypoxia-Inducible factor 1 (HIF1) upregulation and/or AMP-activated protein kinase activation.ConclusionsFDG may provide useful information about the oxygenation status of cells in hypoxic regions where HIF1 upregulation is hypoxia-driven.

AB - IntroductionHypoxia can stimulate 18F-fluorodeoxyglucose (FDG) uptake in cultured cells. A better understanding of the underlying molecular mechanism is required to determine the value of FDG for studying tumour hypoxia.MethodsThe effect of hypoxia on FDG uptake, and key proteins involved in glucose transport and glycolysis, was studied in MCF7 and MDA231 breast cancer cell lines.ResultsHypoxia induced a dose- and time-dependent increase in FDG uptake. The FDG increase was transient, suggesting that FDG uptake is only likely to be increased by acute hypoxia (< 24 h). Molecular analysis indicated that hypoxia upregulated glut1 and 6-phosphofructo-2-kinase, key proteins involved in regulating glucose transport and glycolysis, and that these changes were induced by Hypoxia-Inducible factor 1 (HIF1) upregulation and/or AMP-activated protein kinase activation.ConclusionsFDG may provide useful information about the oxygenation status of cells in hypoxic regions where HIF1 upregulation is hypoxia-driven.

KW - AMP-activated protein kinase

KW - fluorodeoxyglucose

KW - hypoxia

KW - hypoxia-inducible factor 1

KW - oncology

KW - positron emission tomography

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