Candida glabrata susceptibility to antifungals and phagocytosis is modulated by acetate

Sandra Mota, Rosana Alves, Catarina Carneiro, Sónia Silva, Alistair J. Brown, Fabian Istel, Karl Kuchler, Paula Sampaio, Margarida Casal, Mariana Henriques, Sandra Paiva*

*Corresponding author for this work

Research output: Contribution to journalArticle

14 Citations (Scopus)
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Abstract

Candida glabrata is considered a major opportunistic fungal pathogen of humans. The capacity of this yeast species to cause infections is dependent on the ability to grow within the human host environment and to assimilate the carbon sources available. Previous studies have suggested that C. albicans can encounter glucose-poor microenvironments during infection and that the ability to use alternative non-fermentable carbon sources, such as carboxylic acids, contributes to the virulence of this fungus. Transcriptional studies on C. glabrata cells identified a similar response, upon nutrient deprivation. In this work, we aimed at analyzing biofilm formation, antifungal drug resistance, and phagocytosis of C. glabrata cells grown in the presence of acetic acid as an alternative carbon source. C. glabrata planktonic cells grown in media containing acetic acid were more susceptible to fluconazole and were better phagocytosed and killed by macrophages than when compared to media lacking acetic acid. Growth in acetic acid also affected the ability of C. glabrata to form biofilms. The genes ADY2a, ADY2b, FPS1, FPS2, and ATO3, encoding putative carboxylate transporters, were upregulated in C. glabrata planktonic and biofilm cells in the presence of acetic acid. Phagocytosis assays with fps1 and ady2a mutant strains suggested a potential role of FPS1 and ADY2a in the phagocytosis process. These results highlight how acidic pH niches, associated with the presence of acetic acid, can impact in the treatment of C. glabrata infections, in particular in vaginal candidiasis.

Original languageEnglish
Article number919
JournalFrontiers in Microbiology
Volume6
DOIs
Publication statusPublished - 4 Sep 2015

Fingerprint

Candida glabrata
Phagocytosis
Acetic Acid
Acetates
Biofilms
Carbon
Fungal Drug Resistance
Infection
Fluconazole
Candidiasis
Carboxylic Acids
Virulence
Fungi
Yeasts
Macrophages
Glucose
Food
Growth

Keywords

  • Acetate
  • Antifungal drug resistance
  • Candida glabrata
  • Candidiasis
  • Fluconazole
  • Phagocytosis
  • Transporters

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Mota, S., Alves, R., Carneiro, C., Silva, S., Brown, A. J., Istel, F., ... Paiva, S. (2015). Candida glabrata susceptibility to antifungals and phagocytosis is modulated by acetate. Frontiers in Microbiology, 6, [919]. https://doi.org/10.3389/fmicb.2015.00919

Candida glabrata susceptibility to antifungals and phagocytosis is modulated by acetate. / Mota, Sandra; Alves, Rosana; Carneiro, Catarina; Silva, Sónia; Brown, Alistair J.; Istel, Fabian; Kuchler, Karl; Sampaio, Paula; Casal, Margarida; Henriques, Mariana; Paiva, Sandra.

In: Frontiers in Microbiology, Vol. 6, 919, 04.09.2015.

Research output: Contribution to journalArticle

Mota, S, Alves, R, Carneiro, C, Silva, S, Brown, AJ, Istel, F, Kuchler, K, Sampaio, P, Casal, M, Henriques, M & Paiva, S 2015, 'Candida glabrata susceptibility to antifungals and phagocytosis is modulated by acetate', Frontiers in Microbiology, vol. 6, 919. https://doi.org/10.3389/fmicb.2015.00919
Mota, Sandra ; Alves, Rosana ; Carneiro, Catarina ; Silva, Sónia ; Brown, Alistair J. ; Istel, Fabian ; Kuchler, Karl ; Sampaio, Paula ; Casal, Margarida ; Henriques, Mariana ; Paiva, Sandra. / Candida glabrata susceptibility to antifungals and phagocytosis is modulated by acetate. In: Frontiers in Microbiology. 2015 ; Vol. 6.
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AU - Istel, Fabian

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