Candida orthopsilosis and Candida metapsilosis, spp. nov., to replace Candida parapsilosis groups II and III

Arianna Tavanti, Amanda Denise Davidson, Neil Andrew Robert Gow, M. C. Maiden, Frank Christopher Odds

Research output: Contribution to journalArticle

392 Citations (Scopus)

Abstract

Two new species, Candida orthopsilosis and C. metapsilosis, are proposed to replace the existing designations of C parapsilosis groups II and III, respectively. The species C. parapsilosis is retained for group I isolates. Attempts to construct a multilocus sequence typing scheme to differentiate individual strains of C. parapsilosis instead revealed fixed DNA sequence differences between pairs of subgroups in four genes: COX3, L1A1, SADH, and SYAL PCR amplicons for sequencing were obtained for these four plus a further seven genes from 21 group I isolates. For nine group II isolates, PCR products were obtained from only 5 of the 11 genes, and for two group III isolates PCR products were obtained from a different set of 5 genes. Three of the PCR products from group II and III isolates differed in size from the group I products. Cluster analysis of sequence polymorphisms from COX3, SADH, and SYA1, which were common to the three groups, consistently separated the isolates into three distinct sets. All of these differences, together with DNA sequence similarities <90% in the ITS1 sequence, suggest the subgroups should be afforded species status. The near absence of DNA sequence variability among isolates of C parapsilosis and relatively high levels of sequence variability among isolates of C. orthopsilosis suggest that the former species may have evolved very recently from the latter.

Original languageEnglish
Pages (from-to)284-292
Number of pages8
JournalJournal of Clinical Microbiology
Volume43
DOIs
Publication statusPublished - 2005

Keywords

  • BLOOD-STREAM INFECTIONS
  • ASCOMYCETOUS YEASTS
  • DNA
  • IDENTIFICATION
  • ALBICANS
  • PATHOGENICITY
  • SURVEILLANCE
  • DUBLINIENSIS
  • PROGRAM
  • GENE

Cite this

Candida orthopsilosis and Candida metapsilosis, spp. nov., to replace Candida parapsilosis groups II and III. / Tavanti, Arianna; Davidson, Amanda Denise; Gow, Neil Andrew Robert; Maiden, M. C.; Odds, Frank Christopher.

In: Journal of Clinical Microbiology, Vol. 43, 2005, p. 284-292.

Research output: Contribution to journalArticle

Tavanti, Arianna ; Davidson, Amanda Denise ; Gow, Neil Andrew Robert ; Maiden, M. C. ; Odds, Frank Christopher. / Candida orthopsilosis and Candida metapsilosis, spp. nov., to replace Candida parapsilosis groups II and III. In: Journal of Clinical Microbiology. 2005 ; Vol. 43. pp. 284-292.
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