Cyrodactylus derjavini infection elicits IL-1b expression in rainbow trout skin

Research output: Contribution to journalArticle

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Abstract

Specific gene expression of the pro-inflammatory cytokine IL-1beta and the type II IL-1beta receptor (IL-1RII, 'decoy receptors) was studied in skin of rainbow trout Oncorhynchus mykiss (Walbaum, 1792) fry during infection with the ectoparasitic monogenean Gyrodactylus derjavini Mikailov, 1975. Cytokine expression was studied in both primary and secondary infections using a semi-quantitative RT-PCR technique. Generally, low levels of specific IL-1beta1, IL-1beta2 and IL-1RII gene transcription were found in uninfected hosts. In contrast, a clear induction of both IL-1beta isoforms could be observed during the initial phases of primary G. derjavini infections. A less obvious induction of IL-1beta expression was seen in secondary infections imposed just after recovery from the priming infection. However, challenge infections given one-month post full recovery of the primary infections did not elicit transcription levels above those seen in skin samples from uninfected hosts. Despite this, hosts carrying a secondary infection elicited a stronger response toward G derjavini thus expelling parasites quicker than their naive counterparts. When induced, indications of a more tight regulation of the IL-1beta1 isoform compared to IL-1beta2 could be observed. Increased expression of IL-1RII was seen later during the course of a primary infection and could be involved in the observed down regulation of IL-1, especially the beta1 isoform. The current study represents the first example of cytokine expression in fish induced by an ectoparasitic infection and indicates the importance of localised mucosal immune reactions in responses of fish towards gyroclactylids. (C) 2003 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)107-115
Number of pages8
JournalFish & Shellfish Immunology
Volume15
DOIs
Publication statusPublished - 2003

Keywords

  • Gyrodactylus derjavini
  • rainbow trout (Oncorhynchus mykiss)
  • interleukin-1
  • expression
  • skin
  • RT-PCR
  • ONCORHYNCHUS-MYKISS
  • EPITHELIAL-CELLS
  • INTERLEUKIN-1
  • PLATYHELMINTHES
  • MONOGENEA
  • CLONING
  • GENE
  • IL-1
  • MECHANISMS
  • COMPLEMENT

Cite this

Cyrodactylus derjavini infection elicits IL-1b expression in rainbow trout skin. / Secombes, Christopher John.

In: Fish & Shellfish Immunology, Vol. 15, 2003, p. 107-115.

Research output: Contribution to journalArticle

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abstract = "Specific gene expression of the pro-inflammatory cytokine IL-1beta and the type II IL-1beta receptor (IL-1RII, 'decoy receptors) was studied in skin of rainbow trout Oncorhynchus mykiss (Walbaum, 1792) fry during infection with the ectoparasitic monogenean Gyrodactylus derjavini Mikailov, 1975. Cytokine expression was studied in both primary and secondary infections using a semi-quantitative RT-PCR technique. Generally, low levels of specific IL-1beta1, IL-1beta2 and IL-1RII gene transcription were found in uninfected hosts. In contrast, a clear induction of both IL-1beta isoforms could be observed during the initial phases of primary G. derjavini infections. A less obvious induction of IL-1beta expression was seen in secondary infections imposed just after recovery from the priming infection. However, challenge infections given one-month post full recovery of the primary infections did not elicit transcription levels above those seen in skin samples from uninfected hosts. Despite this, hosts carrying a secondary infection elicited a stronger response toward G derjavini thus expelling parasites quicker than their naive counterparts. When induced, indications of a more tight regulation of the IL-1beta1 isoform compared to IL-1beta2 could be observed. Increased expression of IL-1RII was seen later during the course of a primary infection and could be involved in the observed down regulation of IL-1, especially the beta1 isoform. The current study represents the first example of cytokine expression in fish induced by an ectoparasitic infection and indicates the importance of localised mucosal immune reactions in responses of fish towards gyroclactylids. (C) 2003 Elsevier Ltd. All rights reserved.",
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T1 - Cyrodactylus derjavini infection elicits IL-1b expression in rainbow trout skin

AU - Secombes, Christopher John

PY - 2003

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N2 - Specific gene expression of the pro-inflammatory cytokine IL-1beta and the type II IL-1beta receptor (IL-1RII, 'decoy receptors) was studied in skin of rainbow trout Oncorhynchus mykiss (Walbaum, 1792) fry during infection with the ectoparasitic monogenean Gyrodactylus derjavini Mikailov, 1975. Cytokine expression was studied in both primary and secondary infections using a semi-quantitative RT-PCR technique. Generally, low levels of specific IL-1beta1, IL-1beta2 and IL-1RII gene transcription were found in uninfected hosts. In contrast, a clear induction of both IL-1beta isoforms could be observed during the initial phases of primary G. derjavini infections. A less obvious induction of IL-1beta expression was seen in secondary infections imposed just after recovery from the priming infection. However, challenge infections given one-month post full recovery of the primary infections did not elicit transcription levels above those seen in skin samples from uninfected hosts. Despite this, hosts carrying a secondary infection elicited a stronger response toward G derjavini thus expelling parasites quicker than their naive counterparts. When induced, indications of a more tight regulation of the IL-1beta1 isoform compared to IL-1beta2 could be observed. Increased expression of IL-1RII was seen later during the course of a primary infection and could be involved in the observed down regulation of IL-1, especially the beta1 isoform. The current study represents the first example of cytokine expression in fish induced by an ectoparasitic infection and indicates the importance of localised mucosal immune reactions in responses of fish towards gyroclactylids. (C) 2003 Elsevier Ltd. All rights reserved.

AB - Specific gene expression of the pro-inflammatory cytokine IL-1beta and the type II IL-1beta receptor (IL-1RII, 'decoy receptors) was studied in skin of rainbow trout Oncorhynchus mykiss (Walbaum, 1792) fry during infection with the ectoparasitic monogenean Gyrodactylus derjavini Mikailov, 1975. Cytokine expression was studied in both primary and secondary infections using a semi-quantitative RT-PCR technique. Generally, low levels of specific IL-1beta1, IL-1beta2 and IL-1RII gene transcription were found in uninfected hosts. In contrast, a clear induction of both IL-1beta isoforms could be observed during the initial phases of primary G. derjavini infections. A less obvious induction of IL-1beta expression was seen in secondary infections imposed just after recovery from the priming infection. However, challenge infections given one-month post full recovery of the primary infections did not elicit transcription levels above those seen in skin samples from uninfected hosts. Despite this, hosts carrying a secondary infection elicited a stronger response toward G derjavini thus expelling parasites quicker than their naive counterparts. When induced, indications of a more tight regulation of the IL-1beta1 isoform compared to IL-1beta2 could be observed. Increased expression of IL-1RII was seen later during the course of a primary infection and could be involved in the observed down regulation of IL-1, especially the beta1 isoform. The current study represents the first example of cytokine expression in fish induced by an ectoparasitic infection and indicates the importance of localised mucosal immune reactions in responses of fish towards gyroclactylids. (C) 2003 Elsevier Ltd. All rights reserved.

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KW - EPITHELIAL-CELLS

KW - INTERLEUKIN-1

KW - PLATYHELMINTHES

KW - MONOGENEA

KW - CLONING

KW - GENE

KW - IL-1

KW - MECHANISMS

KW - COMPLEMENT

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DO - 10.1016/S1050-4648(02)00142-0

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VL - 15

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EP - 115

JO - Fish & Shellfish Immunology

JF - Fish & Shellfish Immunology

SN - 1050-4648

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