Identification and analysis of an interleukin 8-like molecule in rainbow trout Oncorhynchus mykiss

Kerry J Laing, Jun J Zou, Tiehui Wang, Niels Bols, Ikuo Hirono, Takashi Aoki, Christopher J Secombes

Research output: Contribution to journalArticle

155 Citations (Scopus)

Abstract

An interleukin 8 (IL-8) homologue has been identified in the rainbow trout Oncorhynchus mykiss. The transcript contains an open reading frame of 294 nucleotides that translates into a 97 amino acid putative peptide, with 5' and 3' untranslated regions (UTR) of 171 and 453 nucleotides, respectively. As with previously sequenced lamprey and flounder genes, the trout amino acid sequence lacks the typical ELR motif upstream of the first pair of cysteines, where DLR is present. The trout IL-8 gene contains four exons divided by three short introns of 341, 247 and 292bp, and occupies 1824bp of genomic DNA. RT-PCR reveals a low level constitutive expression of the IL-8 homologue in many tissues, including spleen, heart, liver, head kidney and gill. Expression was not detectable in the brain. Whilst no apparent affect of lipopolysaccharide (LPS) on IL-8 expression was observed in vivo, stimulation of a trout macrophage cell line (RTS-11) with either LPS or poly I:C did result in clear up-regulation of IL-8 expression, detectable by RT-PCR and Northern blot analysis.
Original languageEnglish
Pages (from-to)433-44
Number of pages12
JournalDevelopmental and Comparative Immunology
Volume26
Issue number5
DOIs
Publication statusPublished - Jun 2002

Keywords

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Cloning, Molecular
  • DNA, Complementary
  • Gene Expression Regulation
  • Gene Library
  • Humans
  • Interleukin-8
  • Molecular Sequence Data
  • Oncorhynchus mykiss
  • Phylogeny
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Homology, Amino Acid

Fingerprint Dive into the research topics of 'Identification and analysis of an interleukin 8-like molecule in rainbow trout Oncorhynchus mykiss'. Together they form a unique fingerprint.

Cite this