Identification and characterisation of 17 polymorphic candidate genes for response to parasitic nematode (Trichostrongylus tenuis) infection in red grouse (Lagopus lagopus scotica)

Marius Alexander Wenzel, Lucy M. I. Webster, Steve Paterson, Stuart B. Piertney

Research output: Contribution to journalArticle

3 Citations (Scopus)
5 Downloads (Pure)

Abstract

The red grouse (Lagopus lagopus scotica) is an economically important game bird species endemic to the upland heather moors of the British Isles, where its conservation status is "amber" due to long-term declines in breeding populations. One major driver of grouse population ecology is chronic infection by the highly prevalent, gastrointestinal parasitic nematode Trichostrongylus tenuis. Here, we outline the identification and characterisation of 17 candidate genes for the physiological response of red grouse to parasite infection, developed de novo from functional and genetic analysis of grouse transcriptomic and genomic resources. These genes capture broad physiological functions, including immune system processes, xenobiotics detoxification, oxidative balance, metabolism and cell cycle regulation. All genes were polymorphic at the landscape scale in north-east Scotland, indicating great utility for characterising the causes and consequences of spatio-temporal genetic variation in relation to parasite-mediated eco-evolutionary processes in red grouse populations.

Original languageEnglish
Pages (from-to)23-28
Number of pages6
JournalConservation Genetics Resources
Volume7
Issue number1
Early online date31 Aug 2014
DOIs
Publication statusPublished - Mar 2015

Keywords

  • red grouse
  • lagopus
  • parasite
  • candidate genes
  • adaptive genetic diversity
  • population genomics
  • population-dynamics

Cite this

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title = "Identification and characterisation of 17 polymorphic candidate genes for response to parasitic nematode (Trichostrongylus tenuis) infection in red grouse (Lagopus lagopus scotica)",
abstract = "The red grouse (Lagopus lagopus scotica) is an economically important game bird species endemic to the upland heather moors of the British Isles, where its conservation status is {"}amber{"} due to long-term declines in breeding populations. One major driver of grouse population ecology is chronic infection by the highly prevalent, gastrointestinal parasitic nematode Trichostrongylus tenuis. Here, we outline the identification and characterisation of 17 candidate genes for the physiological response of red grouse to parasite infection, developed de novo from functional and genetic analysis of grouse transcriptomic and genomic resources. These genes capture broad physiological functions, including immune system processes, xenobiotics detoxification, oxidative balance, metabolism and cell cycle regulation. All genes were polymorphic at the landscape scale in north-east Scotland, indicating great utility for characterising the causes and consequences of spatio-temporal genetic variation in relation to parasite-mediated eco-evolutionary processes in red grouse populations.",
keywords = "red grouse, lagopus, parasite, candidate genes, adaptive genetic diversity, population genomics, population-dynamics",
author = "Wenzel, {Marius Alexander} and Webster, {Lucy M. I.} and Steve Paterson and Piertney, {Stuart B.}",
note = "Acknowledgements This study was funded by a BBSRC studentship (MA Wenzel) and NERC Grants NE/H00775X/1 and NE/D000602/1 (SB Piertney). We are grateful to Jacob Hoglund for providing willow grouse samples, Mario Roder, Keliya Bai, Marianne James, Matt Oliver, Gill Murray-Dickson, Francois Mougeot and Jesus Martınez-Padilla for help with fieldwork, and all grouse estate factors, owners and keepers, most particularly Alistair Mitchell, Shaila Rao, Christopher Murphy, Richard Cooke and Fred Taylor, for providing access to estate game larders.",
year = "2015",
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AU - Paterson, Steve

AU - Piertney, Stuart B.

N1 - Acknowledgements This study was funded by a BBSRC studentship (MA Wenzel) and NERC Grants NE/H00775X/1 and NE/D000602/1 (SB Piertney). We are grateful to Jacob Hoglund for providing willow grouse samples, Mario Roder, Keliya Bai, Marianne James, Matt Oliver, Gill Murray-Dickson, Francois Mougeot and Jesus Martınez-Padilla for help with fieldwork, and all grouse estate factors, owners and keepers, most particularly Alistair Mitchell, Shaila Rao, Christopher Murphy, Richard Cooke and Fred Taylor, for providing access to estate game larders.

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N2 - The red grouse (Lagopus lagopus scotica) is an economically important game bird species endemic to the upland heather moors of the British Isles, where its conservation status is "amber" due to long-term declines in breeding populations. One major driver of grouse population ecology is chronic infection by the highly prevalent, gastrointestinal parasitic nematode Trichostrongylus tenuis. Here, we outline the identification and characterisation of 17 candidate genes for the physiological response of red grouse to parasite infection, developed de novo from functional and genetic analysis of grouse transcriptomic and genomic resources. These genes capture broad physiological functions, including immune system processes, xenobiotics detoxification, oxidative balance, metabolism and cell cycle regulation. All genes were polymorphic at the landscape scale in north-east Scotland, indicating great utility for characterising the causes and consequences of spatio-temporal genetic variation in relation to parasite-mediated eco-evolutionary processes in red grouse populations.

AB - The red grouse (Lagopus lagopus scotica) is an economically important game bird species endemic to the upland heather moors of the British Isles, where its conservation status is "amber" due to long-term declines in breeding populations. One major driver of grouse population ecology is chronic infection by the highly prevalent, gastrointestinal parasitic nematode Trichostrongylus tenuis. Here, we outline the identification and characterisation of 17 candidate genes for the physiological response of red grouse to parasite infection, developed de novo from functional and genetic analysis of grouse transcriptomic and genomic resources. These genes capture broad physiological functions, including immune system processes, xenobiotics detoxification, oxidative balance, metabolism and cell cycle regulation. All genes were polymorphic at the landscape scale in north-east Scotland, indicating great utility for characterising the causes and consequences of spatio-temporal genetic variation in relation to parasite-mediated eco-evolutionary processes in red grouse populations.

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