Identification and characterization of the actinomycin G gene cluster in Streptomyces iakyrus

Xiaoling Wang; Jioji Tabudravu; Mostafa Rateb; Krystal Joan Annand; Zhiwei Qin; Marcel Jaspars; Zixin Deng; Yi  Yu; Hai Deng

doi:10.1039/c3mb70081j

Identification and characterization of the actinomycin G gene cluster in Streptomyces iakyrus

Xiaoling Wang, Jioji Tabudravu, Mostafa Rateb, Krystal Joan Annand, Zhiwei Qin, Marcel Jaspars, Zixin Deng, Yi Yu, Hai Deng

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

The gene cluster directing actinomycin G biosynthesis in Streptomyces iakyrus has been identified and sequenced. It contains one actinomycin synthetase I (ACMS I) gene and two copies of ACMS II and III genes. Genetic analysis demonstrates a unique partnership between the putative hydroxylation and chlorination activities as both acmG8 and acmG9 genes need to be transcribed for the biosynthesis of actinomycin G2-3, respectively.

Original language	English
Pages (from-to)	1286-1289
Number of pages	4
Journal	Molecular BioSystems
Volume	9
Issue number	6
Early online date	25 Mar 2013
DOIs	https://doi.org/10.1039/c3mb70081j
Publication status	Published - Jun 2013

Keywords

anti-bacterial agents
base sequence
DNA, bacterial
dactinomycin
genes, bacterial
halogenation
hydroxylation
molecular sequence data
multienzyme complexes
multigene family
nucleotidyltransferases
peptide synthases
streptomyces

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10.1039/c3mb70081j

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title = "Identification and characterization of the actinomycin G gene cluster in Streptomyces iakyrus",

abstract = "The gene cluster directing actinomycin G biosynthesis in Streptomyces iakyrus has been identified and sequenced. It contains one actinomycin synthetase I (ACMS I) gene and two copies of ACMS II and III genes. Genetic analysis demonstrates a unique partnership between the putative hydroxylation and chlorination activities as both acmG8 and acmG9 genes need to be transcribed for the biosynthesis of actinomycin G2-3, respectively.",

keywords = "anti-bacterial agents, base sequence, DNA, bacterial, dactinomycin, genes, bacterial, halogenation, hydroxylation, molecular sequence data, multienzyme complexes, multigene family, nucleotidyltransferases, peptide synthases, streptomyces",

author = "Xiaoling Wang and Jioji Tabudravu and Mostafa Rateb and Annand, {Krystal Joan} and Zhiwei Qin and Marcel Jaspars and Zixin Deng and Yi Yu and Hai Deng",

year = "2013",

month = jun,

doi = "10.1039/c3mb70081j",

language = "English",

volume = "9",

pages = "1286--1289",

journal = "Molecular BioSystems",

issn = "1742-206X",

publisher = "Royal Society of Chemistry",

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TY - JOUR

T1 - Identification and characterization of the actinomycin G gene cluster in Streptomyces iakyrus

AU - Wang, Xiaoling

AU - Tabudravu, Jioji

AU - Rateb, Mostafa

AU - Annand, Krystal Joan

AU - Qin, Zhiwei

AU - Jaspars, Marcel

AU - Deng, Zixin

AU - Yu, Yi

AU - Deng, Hai

PY - 2013/6

Y1 - 2013/6

N2 - The gene cluster directing actinomycin G biosynthesis in Streptomyces iakyrus has been identified and sequenced. It contains one actinomycin synthetase I (ACMS I) gene and two copies of ACMS II and III genes. Genetic analysis demonstrates a unique partnership between the putative hydroxylation and chlorination activities as both acmG8 and acmG9 genes need to be transcribed for the biosynthesis of actinomycin G2-3, respectively.

AB - The gene cluster directing actinomycin G biosynthesis in Streptomyces iakyrus has been identified and sequenced. It contains one actinomycin synthetase I (ACMS I) gene and two copies of ACMS II and III genes. Genetic analysis demonstrates a unique partnership between the putative hydroxylation and chlorination activities as both acmG8 and acmG9 genes need to be transcribed for the biosynthesis of actinomycin G2-3, respectively.

KW - anti-bacterial agents

KW - base sequence

KW - DNA, bacterial

KW - dactinomycin

KW - genes, bacterial

KW - halogenation

KW - hydroxylation

KW - molecular sequence data

KW - multienzyme complexes

KW - multigene family

KW - nucleotidyltransferases

KW - peptide synthases

KW - streptomyces

U2 - 10.1039/c3mb70081j

DO - 10.1039/c3mb70081j

M3 - Article

C2 - 23567908

SN - 1742-206X

VL - 9

SP - 1286

EP - 1289

JO - Molecular BioSystems

JF - Molecular BioSystems

IS - 6

ER -

Identification and characterization of the actinomycin G gene cluster in Streptomyces iakyrus

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Keywords

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