Abstract
| Original language | English |
|---|---|
| Article number | 103454 |
| Number of pages | 9 |
| Journal | Developmental and Comparative Immunology |
| Volume | 101 |
| Early online date | 19 Jul 2019 |
| DOIs | |
| Publication status | Published - Dec 2019 |
Fingerprint
Keywords
- Asian swamp eel (Monopterus albus)
- CXCL11_L3
- CXCL_F2
- Evolution
- Modulation
- MOLECULAR CHARACTERIZATION
- EXPRESSION ANALYSIS
- CATFISH
- GRASS CARP
- CROAKER LARIMICHTHYS-CROCEA
- INTERFERON REGULATORY FACTOR
- GENE
- SEQUENCE
- SUPERFAMILY
- RECEPTORS
ASJC Scopus subject areas
- Immunology
- Developmental Biology
Cite this
Identification and characterization of three CXC chemokines in Asian swamp eel (Monopterus albus) uncover a third CXCL11_like group in fish. / Yuan, Hanwen; Li, Youshen; Han, Panpan; Tian, Guangming; Zhang, Wenbing; Guo, Huizhi; Xu, Qiaoqing (Corresponding Author); Wang, Tiehui (Corresponding Author).
In: Developmental and Comparative Immunology, Vol. 101, 103454, 12.2019.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification and characterization of three CXC chemokines in Asian swamp eel (Monopterus albus) uncover a third CXCL11_like group in fish
AU - Yuan, Hanwen
AU - Li, Youshen
AU - Han, Panpan
AU - Tian, Guangming
AU - Zhang, Wenbing
AU - Guo, Huizhi
AU - Xu, Qiaoqing
AU - Wang, Tiehui
N1 - Acknowledgment This study was supported by the Foundation of Guangdong Provincial Key Laboratory of Marine Biotechnology (No. GPKLMB201801), the Fund of Key Laboratory of Aquaculture Disease Control, and the Ministry of Agriculture and National Natural Science Foundation of China (31460689).
PY - 2019/12
Y1 - 2019/12
N2 - Chemokines direct cell migration in development and immune defense, and bridge between innate and adaptive immune responses. The chemokine gene family has been rapidly evolving and has undergone species/lineage-specific expansion. Mammals possess inflammatory CXC chemokines CXCL1-8/15 and CXCL9-11 sub-groups, and homeostatic CXCL12-14, 16–17. Orthologues of mammalian CXCL12-14, three chemokines related to CXCL1-8/15 (CXCL8_L1-3), two chemokines related to CXC9-11 (CXCL11_L1-2), and five fish-specific chemokines (CXCL_F1-5) have been described in teleosts. In this study, we reported three novel CXC chemokines in Asian swamp eel Monopterus albus, a commercially important freshwater fish species in China. Two of them belong to the fish-specific CXCL_F2 group, named CXCL_F2a/b, that share 89.5% amino acid identity. The other (CXCL11_L3) belongs to a third CXCL11_L related to the mammalian CXCL9-11 subfamily found only in percomorph fish species, and is the only CXCL9-11 related molecules in this lineage. Mammalian CXCL9-11 attract Th1 cells, and block the migration of Th2 cells in an immune response. This study suggests that all major lineages of teleosts have a CXCL9-11 related chemokine that will aid future functional investigation of CXCL11_L in fish. Cxcl_f2a is highly expressed constitutively in the skin of swamp eels that may attract immune cells to protect the skin in the absence of scales. Cxcl11_l3 and cxcl_f2b are highly expressed in immune tissues/organs and are up-regulated by the viral mimic poly I:C, but not bacterial infection in vivo, suggesting their role in anti-viral defense. The two cxcl_f2 paralogues are differentially expressed and modulated, indicating sub- and/or neo-functionalization.
AB - Chemokines direct cell migration in development and immune defense, and bridge between innate and adaptive immune responses. The chemokine gene family has been rapidly evolving and has undergone species/lineage-specific expansion. Mammals possess inflammatory CXC chemokines CXCL1-8/15 and CXCL9-11 sub-groups, and homeostatic CXCL12-14, 16–17. Orthologues of mammalian CXCL12-14, three chemokines related to CXCL1-8/15 (CXCL8_L1-3), two chemokines related to CXC9-11 (CXCL11_L1-2), and five fish-specific chemokines (CXCL_F1-5) have been described in teleosts. In this study, we reported three novel CXC chemokines in Asian swamp eel Monopterus albus, a commercially important freshwater fish species in China. Two of them belong to the fish-specific CXCL_F2 group, named CXCL_F2a/b, that share 89.5% amino acid identity. The other (CXCL11_L3) belongs to a third CXCL11_L related to the mammalian CXCL9-11 subfamily found only in percomorph fish species, and is the only CXCL9-11 related molecules in this lineage. Mammalian CXCL9-11 attract Th1 cells, and block the migration of Th2 cells in an immune response. This study suggests that all major lineages of teleosts have a CXCL9-11 related chemokine that will aid future functional investigation of CXCL11_L in fish. Cxcl_f2a is highly expressed constitutively in the skin of swamp eels that may attract immune cells to protect the skin in the absence of scales. Cxcl11_l3 and cxcl_f2b are highly expressed in immune tissues/organs and are up-regulated by the viral mimic poly I:C, but not bacterial infection in vivo, suggesting their role in anti-viral defense. The two cxcl_f2 paralogues are differentially expressed and modulated, indicating sub- and/or neo-functionalization.
KW - Asian swamp eel (Monopterus albus)
KW - CXCL11_L3
KW - CXCL_F2
KW - Evolution
KW - Modulation
KW - MOLECULAR CHARACTERIZATION
KW - EXPRESSION ANALYSIS
KW - CATFISH
KW - GRASS CARP
KW - CROAKER LARIMICHTHYS-CROCEA
KW - INTERFERON REGULATORY FACTOR
KW - GENE
KW - SEQUENCE
KW - SUPERFAMILY
KW - RECEPTORS
UR - http://www.scopus.com/inward/record.url?scp=85069582305&partnerID=8YFLogxK
UR - http://www.mendeley.com/research/identification-characterization-three-cxc-chemokines-asian-swamp-eel-monopterus-albus-uncovers-third
U2 - 10.1016/j.dci.2019.103454
DO - 10.1016/j.dci.2019.103454
M3 - Article
C2 - 31326565
VL - 101
JO - Developmental and Comparative Immunology
JF - Developmental and Comparative Immunology
SN - 0145-305X
M1 - 103454
ER -