Identification and evaluation of vaccine candidate antigens from the poultry red mite (Dermanyssus gallinae)

Kathryn Bartley (Corresponding Author), Harry W. Wright, John F. Huntley, Erin D.T. Manson, Neil F. Inglis, Kevin McLean, Mintu Nath, Yvonne Bartley, Alasdair J. Nisbet

Research output: Contribution to journalArticle

Abstract

An aqueous extract of the haematophagous poultry ectoparasite, Dermanyssus gallinae, was subfractionated using anion exchange chromatography. Six of these subfractions were used to immunise hens and the blood from these hens was fed, in vitro, to poultry red mites. Mite mortality following these feeds was indicative of protective antigens in two of the subfractions, with the risks of mites dying being 3.1 and 3.7 times higher than in the control group (P<0.001). A combination of two-dimensional immunoblotting and immunoaffinity chromatography, using IgY from hens immunised with these subfractions, was used in concert with proteomic analyses to identify the strongest immunogenic proteins in each of these subfractions. Ten of the immunoreactive proteins were selected for assessment as vaccine candidates using the following criteria: intensity of immune recognition; likelihood of exposure of the antigen to the antibodies in a blood meal; proposed function and known vaccine potential of orthologous molecules. Recombinant versions of each of these 10 proteins were produced in Escherichia coli and were used to immunise hens. Subsequent in vitro feeding of mites on blood from these birds indicated that immunisation with Deg-SRP-1 (serpin), Deg-VIT-1 (vitellogenin), Deg-HGP-1 (hemelipoglycoprotein) or Deg-PUF-1 (a protein of unknown function) resulted in significantly increased risk of mite death (1.7-2.8times higher than in mites fed blood from control hens immunised with adjuvant only, P<0.001). The potential for using these antigens in a recombinant vaccine is discussed.

Original languageEnglish
Pages (from-to)819-830
Number of pages12
JournalInternational Journal for Parasitology
Volume45
Issue number13
Early online date19 Aug 2015
DOIs
Publication statusPublished - Nov 2015

Fingerprint

Mites
Poultry
Vaccines
Antigens
Chromatography
Proteins
Serpins
Vitellogenins
Synthetic Vaccines
Immunoblotting
Proteomics
Birds
Anions
Meals
Immunization
Escherichia coli
Control Groups
Mortality
Antibodies

Keywords

  • Dermanyssus gallinae
  • Vaccine
  • Serpin
  • Vitellogenin
  • Hemelipoglycoprotein

Cite this

Bartley, K., Wright, H. W., Huntley, J. F., Manson, E. D. T., Inglis, N. F., McLean, K., ... Nisbet, A. J. (2015). Identification and evaluation of vaccine candidate antigens from the poultry red mite (Dermanyssus gallinae). International Journal for Parasitology, 45(13), 819-830. https://doi.org/10.1016/j.ijpara.2015.07.004

Identification and evaluation of vaccine candidate antigens from the poultry red mite (Dermanyssus gallinae). / Bartley, Kathryn (Corresponding Author); Wright, Harry W.; Huntley, John F.; Manson, Erin D.T.; Inglis, Neil F.; McLean, Kevin; Nath, Mintu; Bartley, Yvonne; Nisbet, Alasdair J.

In: International Journal for Parasitology, Vol. 45, No. 13, 11.2015, p. 819-830.

Research output: Contribution to journalArticle

Bartley, K, Wright, HW, Huntley, JF, Manson, EDT, Inglis, NF, McLean, K, Nath, M, Bartley, Y & Nisbet, AJ 2015, 'Identification and evaluation of vaccine candidate antigens from the poultry red mite (Dermanyssus gallinae)' International Journal for Parasitology, vol. 45, no. 13, pp. 819-830. https://doi.org/10.1016/j.ijpara.2015.07.004
Bartley, Kathryn ; Wright, Harry W. ; Huntley, John F. ; Manson, Erin D.T. ; Inglis, Neil F. ; McLean, Kevin ; Nath, Mintu ; Bartley, Yvonne ; Nisbet, Alasdair J. / Identification and evaluation of vaccine candidate antigens from the poultry red mite (Dermanyssus gallinae). In: International Journal for Parasitology. 2015 ; Vol. 45, No. 13. pp. 819-830.
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abstract = "An aqueous extract of the haematophagous poultry ectoparasite, Dermanyssus gallinae, was subfractionated using anion exchange chromatography. Six of these subfractions were used to immunise hens and the blood from these hens was fed, in vitro, to poultry red mites. Mite mortality following these feeds was indicative of protective antigens in two of the subfractions, with the risks of mites dying being 3.1 and 3.7 times higher than in the control group (P<0.001). A combination of two-dimensional immunoblotting and immunoaffinity chromatography, using IgY from hens immunised with these subfractions, was used in concert with proteomic analyses to identify the strongest immunogenic proteins in each of these subfractions. Ten of the immunoreactive proteins were selected for assessment as vaccine candidates using the following criteria: intensity of immune recognition; likelihood of exposure of the antigen to the antibodies in a blood meal; proposed function and known vaccine potential of orthologous molecules. Recombinant versions of each of these 10 proteins were produced in Escherichia coli and were used to immunise hens. Subsequent in vitro feeding of mites on blood from these birds indicated that immunisation with Deg-SRP-1 (serpin), Deg-VIT-1 (vitellogenin), Deg-HGP-1 (hemelipoglycoprotein) or Deg-PUF-1 (a protein of unknown function) resulted in significantly increased risk of mite death (1.7-2.8times higher than in mites fed blood from control hens immunised with adjuvant only, P<0.001). The potential for using these antigens in a recombinant vaccine is discussed.",
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