Identification and expression modulation of a C-type lectin domain family 4 homologue that is highly expressed in monocytes/macrophages in rainbow trout (Oncorhynchus mykiss)

Petronella Johansson; Tiehui Wang; Bertrand Collet; Yolanda Corripio-Miyar; Milena M. Monte; Christopher J. Secombes; Jun Zou

doi:10.1016/j.dci.2015.08.005

Identification and expression modulation of a C-type lectin domain family 4 homologue that is highly expressed in monocytes/macrophages in rainbow trout (Oncorhynchus mykiss)

Petronella Johansson, Tiehui Wang, Bertrand Collet, Yolanda Corripio-Miyar, Milena M. Monte, Christopher J. Secombes, Jun Zou

MARINE SCOTLAND SCIENCE

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Abstract

The C-type lectin domain containing (CLEC) receptors including CD209 are expressed in vivo by monocytes, monocyte-derived macrophages and dendritic cells and by in vitro generated monocyte-derived cells. This paper reports the cloning and sequencing of a lectin molecule, CLEC4T1, in rainbow trout that is a homologue of the CLEC4 family. The expression pattern of the CLEC4T1 was investigated in vivo after infection with a bacterial pathogen and in cultured macrophages after modulation with microbial mimics. Trout CLEC4T1 was highly expressed in spleen and head kidney following infection with Yersinia ruckeri. Expression could also be induced in macrophage cultures by LPS but not by Poly I:C, and suggests that the regulation of CLEC4T1 expression in trout varies according to the nature of the stimulant. A polyclonal CLEC4T1 antibody was generated and validated by Western blotting for use in evaluation of CLEC4T1+ cells by flow cytometry analysis. Freshly isolated adherent trout head kidney cultures, potentially containing macrophages and dendritic cell precursors, showed an increase of CLEC4T1+ cells (assessed by flow cytometry) upon stimulation with recombinant interleukin-4/13A. The results suggest that CLEC4T1 is a useful marker for further characterisation of monocyte derived antigen presenting cells in fish.

Original language	English
Pages (from-to)	55-65
Number of pages	11
Journal	Developmental and Comparative Immunology
Volume	54
Issue number	1
Early online date	13 Aug 2015
DOIs	https://doi.org/10.1016/j.dci.2015.08.005
Publication status	Published - Jan 2016

Bibliographical note

Acknowledgements
PJ was supported by a PhD studentship from the Marine Alliance for Science and Technology for Scotland (MASTS), and TW was supported by a MASTS postdoctoral fellowship.

Keywords

dendritic cell
C-type lectin
CLEC4/CD209
rainbow trout
interleukin-4/13A
innate immunity
macrophage

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Johansson, P., Wang, T., Collet, B., Corripio-Miyar, Y., Monte, M. M., Secombes, C. J., & Zou, J. (2016). Identification and expression modulation of a C-type lectin domain family 4 homologue that is highly expressed in monocytes/macrophages in rainbow trout (Oncorhynchus mykiss). Developmental and Comparative Immunology, 54(1), 55-65. https://doi.org/10.1016/j.dci.2015.08.005

Identification and expression modulation of a C-type lectin domain family 4 homologue that is highly expressed in monocytes/macrophages in rainbow trout (Oncorhynchus mykiss). / Johansson, Petronella; Wang, Tiehui; Collet, Bertrand et al.
In: Developmental and Comparative Immunology, Vol. 54, No. 1, 01.2016, p. 55-65.

Research output: Contribution to journal › Article › peer-review

Johansson, P, Wang, T, Collet, B, Corripio-Miyar, Y, Monte, MM, Secombes, CJ & Zou, J 2016, 'Identification and expression modulation of a C-type lectin domain family 4 homologue that is highly expressed in monocytes/macrophages in rainbow trout (Oncorhynchus mykiss)', Developmental and Comparative Immunology, vol. 54, no. 1, pp. 55-65. https://doi.org/10.1016/j.dci.2015.08.005

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abstract = "The C-type lectin domain containing (CLEC) receptors including CD209 are expressed in vivo by monocytes, monocyte-derived macrophages and dendritic cells and by in vitro generated monocyte-derived cells. This paper reports the cloning and sequencing of a lectin molecule, CLEC4T1, in rainbow trout that is a homologue of the CLEC4 family. The expression pattern of the CLEC4T1 was investigated in vivo after infection with a bacterial pathogen and in cultured macrophages after modulation with microbial mimics. Trout CLEC4T1 was highly expressed in spleen and head kidney following infection with Yersinia ruckeri. Expression could also be induced in macrophage cultures by LPS but not by Poly I:C, and suggests that the regulation of CLEC4T1 expression in trout varies according to the nature of the stimulant. A polyclonal CLEC4T1 antibody was generated and validated by Western blotting for use in evaluation of CLEC4T1+ cells by flow cytometry analysis. Freshly isolated adherent trout head kidney cultures, potentially containing macrophages and dendritic cell precursors, showed an increase of CLEC4T1+ cells (assessed by flow cytometry) upon stimulation with recombinant interleukin-4/13A. The results suggest that CLEC4T1 is a useful marker for further characterisation of monocyte derived antigen presenting cells in fish.",

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author = "Petronella Johansson and Tiehui Wang and Bertrand Collet and Yolanda Corripio-Miyar and Monte, {Milena M.} and Secombes, {Christopher J.} and Jun Zou",

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AU - Corripio-Miyar, Yolanda

AU - Monte, Milena M.

AU - Secombes, Christopher J.

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AB - The C-type lectin domain containing (CLEC) receptors including CD209 are expressed in vivo by monocytes, monocyte-derived macrophages and dendritic cells and by in vitro generated monocyte-derived cells. This paper reports the cloning and sequencing of a lectin molecule, CLEC4T1, in rainbow trout that is a homologue of the CLEC4 family. The expression pattern of the CLEC4T1 was investigated in vivo after infection with a bacterial pathogen and in cultured macrophages after modulation with microbial mimics. Trout CLEC4T1 was highly expressed in spleen and head kidney following infection with Yersinia ruckeri. Expression could also be induced in macrophage cultures by LPS but not by Poly I:C, and suggests that the regulation of CLEC4T1 expression in trout varies according to the nature of the stimulant. A polyclonal CLEC4T1 antibody was generated and validated by Western blotting for use in evaluation of CLEC4T1+ cells by flow cytometry analysis. Freshly isolated adherent trout head kidney cultures, potentially containing macrophages and dendritic cell precursors, showed an increase of CLEC4T1+ cells (assessed by flow cytometry) upon stimulation with recombinant interleukin-4/13A. The results suggest that CLEC4T1 is a useful marker for further characterisation of monocyte derived antigen presenting cells in fish.

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KW - rainbow trout

KW - interleukin-4/13A

KW - innate immunity

KW - macrophage

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Identification and expression modulation of a C-type lectin domain family 4 homologue that is highly expressed in monocytes/macrophages in rainbow trout (Oncorhynchus mykiss)

Abstract

Bibliographical note

Keywords

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