Identification and pathogenicity of Fusarium species associated with sesame (Sesamum indicum L.) seeds from the Punjab, Pakistan

Brian Gagosh Nayyar; Steve Woodward; Luis A.J. Mur; Abida Akram; Muhammad Arshad; S.M. Saqlan Naqvi; Shaista Akhund

doi:10.1016/j.pmpp.2018.02.001

Identification and pathogenicity of Fusarium species associated with sesame (Sesamum indicum L.) seeds from the Punjab, Pakistan

Brian Gagosh Nayyar, Steve Woodward, Luis A.J. Mur, Abida Akram, Muhammad Arshad, S.M. Saqlan Naqvi, Shaista Akhund

Aberdeen Centre For Environmental Sustainability

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Sesame (Sesamum indicum) is an oil-crop in Asia and Africa and is widely grown in the Punjab region of Pakistan. A total of 105 sesame seed samples were collected from different locations in the Punjab from which 520 isolates of Fusarium spp. were recovered. These isolates were initially grouped and identified based on morphological characteristics. The identities of representatives of the three most frequently isolated groups (strains designated F01, F98, F153) were identified as Fusarium proliferatum, on the basis of the sequencing of ITS of rDNA and translation elongation factor (TEF-1α) gene regions. Phylogenetic trees generated using the maximum likelihood method showed that these three isolates and a F. proliferatum reference sequence grouped in the same clade with F. phyllophilum, the most closely related species. Pathogenicity tests demonstrated that these three isolates caused disease on sesame plants. Disease Incidence (DI) and Disease Severity Index (DSI) data indicated that F01 was the most virulent isolate, with DI and DSI of approximately 70%. Culture filtrates of F01 reduced sesame seed germination (to 40%) and vigor (to 16.5%) of sesame seedlings. This baseline study suggests that F. proliferatum infection of sesame seeds could be a major source of yield loss in the Punjab, Pakistan which requires further attention.

Original language	English
Pages (from-to)	128 - 135
Number of pages	8
Journal	Physiological & Molecular Plant Pathology
Volume	102
Early online date	3 Feb 2018
DOIs	https://doi.org/10.1016/j.pmpp.2018.02.001
Publication status	Published - 30 Apr 2018

Bibliographical note

This work is a part of Ph.D. project of the first author and financially supported by the Higher Education Commission (HEC), Pakistan under the National Research Program for Universities (NRPU) project (No. 20-5166). Moreover, HEC is also acknowledged for providing funds under International Research Support Initiative Program (IRSIP) to the first author (No. 1-8/HEC/HRD/20143411) to visit the Department of Plant and Soil Science, Institute of Biological and Environmental Sciences, University of Aberdeen, Scotland, UK. The first two authors are thankful to the Govt. of Scotland for providing the import license for three Fusarium isolates under Directive 2008/61/EC and Scottish License No. PH/9/2015.

Keywords

Sesame
Blotter paper method
ITS
TEF-1α

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title = "Identification and pathogenicity of Fusarium species associated with sesame (Sesamum indicum L.) seeds from the Punjab, Pakistan",

abstract = "Sesame (Sesamum indicum) is an oil-crop in Asia and Africa and is widely grown in the Punjab region of Pakistan. A total of 105 sesame seed samples were collected from different locations in the Punjab from which 520 isolates of Fusarium spp. were recovered. These isolates were initially grouped and identified based on morphological characteristics. The identities of representatives of the three most frequently isolated groups (strains designated F01, F98, F153) were identified as Fusarium proliferatum, on the basis of the sequencing of ITS of rDNA and translation elongation factor (TEF-1α) gene regions. Phylogenetic trees generated using the maximum likelihood method showed that these three isolates and a F. proliferatum reference sequence grouped in the same clade with F. phyllophilum, the most closely related species. Pathogenicity tests demonstrated that these three isolates caused disease on sesame plants. Disease Incidence (DI) and Disease Severity Index (DSI) data indicated that F01 was the most virulent isolate, with DI and DSI of approximately 70%. Culture filtrates of F01 reduced sesame seed germination (to 40%) and vigor (to 16.5%) of sesame seedlings. This baseline study suggests that F. proliferatum infection of sesame seeds could be a major source of yield loss in the Punjab, Pakistan which requires further attention.",

keywords = "Sesame, Blotter paper method, ITS, TEF-1α ",

author = "Nayyar, {Brian Gagosh} and Steve Woodward and Mur, {Luis A.J.} and Abida Akram and Muhammad Arshad and Naqvi, {S.M. Saqlan} and Shaista Akhund",

note = "This work is a part of Ph.D. project of the first author and financially supported by the Higher Education Commission (HEC), Pakistan under the National Research Program for Universities (NRPU) project (No. 20-5166). Moreover, HEC is also acknowledged for providing funds under International Research Support Initiative Program (IRSIP) to the first author (No. 1-8/HEC/HRD/20143411) to visit the Department of Plant and Soil Science, Institute of Biological and Environmental Sciences, University of Aberdeen, Scotland, UK. The first two authors are thankful to the Govt. of Scotland for providing the import license for three Fusarium isolates under Directive 2008/61/EC and Scottish License No. PH/9/2015.",

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AU - Nayyar, Brian Gagosh

AU - Woodward, Steve

AU - Mur, Luis A.J.

AU - Akram, Abida

AU - Arshad, Muhammad

AU - Naqvi, S.M. Saqlan

AU - Akhund, Shaista

N1 - This work is a part of Ph.D. project of the first author and financially supported by the Higher Education Commission (HEC), Pakistan under the National Research Program for Universities (NRPU) project (No. 20-5166). Moreover, HEC is also acknowledged for providing funds under International Research Support Initiative Program (IRSIP) to the first author (No. 1-8/HEC/HRD/20143411) to visit the Department of Plant and Soil Science, Institute of Biological and Environmental Sciences, University of Aberdeen, Scotland, UK. The first two authors are thankful to the Govt. of Scotland for providing the import license for three Fusarium isolates under Directive 2008/61/EC and Scottish License No. PH/9/2015.

PY - 2018/4/30

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N2 - Sesame (Sesamum indicum) is an oil-crop in Asia and Africa and is widely grown in the Punjab region of Pakistan. A total of 105 sesame seed samples were collected from different locations in the Punjab from which 520 isolates of Fusarium spp. were recovered. These isolates were initially grouped and identified based on morphological characteristics. The identities of representatives of the three most frequently isolated groups (strains designated F01, F98, F153) were identified as Fusarium proliferatum, on the basis of the sequencing of ITS of rDNA and translation elongation factor (TEF-1α) gene regions. Phylogenetic trees generated using the maximum likelihood method showed that these three isolates and a F. proliferatum reference sequence grouped in the same clade with F. phyllophilum, the most closely related species. Pathogenicity tests demonstrated that these three isolates caused disease on sesame plants. Disease Incidence (DI) and Disease Severity Index (DSI) data indicated that F01 was the most virulent isolate, with DI and DSI of approximately 70%. Culture filtrates of F01 reduced sesame seed germination (to 40%) and vigor (to 16.5%) of sesame seedlings. This baseline study suggests that F. proliferatum infection of sesame seeds could be a major source of yield loss in the Punjab, Pakistan which requires further attention.

AB - Sesame (Sesamum indicum) is an oil-crop in Asia and Africa and is widely grown in the Punjab region of Pakistan. A total of 105 sesame seed samples were collected from different locations in the Punjab from which 520 isolates of Fusarium spp. were recovered. These isolates were initially grouped and identified based on morphological characteristics. The identities of representatives of the three most frequently isolated groups (strains designated F01, F98, F153) were identified as Fusarium proliferatum, on the basis of the sequencing of ITS of rDNA and translation elongation factor (TEF-1α) gene regions. Phylogenetic trees generated using the maximum likelihood method showed that these three isolates and a F. proliferatum reference sequence grouped in the same clade with F. phyllophilum, the most closely related species. Pathogenicity tests demonstrated that these three isolates caused disease on sesame plants. Disease Incidence (DI) and Disease Severity Index (DSI) data indicated that F01 was the most virulent isolate, with DI and DSI of approximately 70%. Culture filtrates of F01 reduced sesame seed germination (to 40%) and vigor (to 16.5%) of sesame seedlings. This baseline study suggests that F. proliferatum infection of sesame seeds could be a major source of yield loss in the Punjab, Pakistan which requires further attention.

KW - Sesame

KW - Blotter paper method

KW - ITS

KW - TEF-1α

U2 - 10.1016/j.pmpp.2018.02.001

DO - 10.1016/j.pmpp.2018.02.001

M3 - Article

SN - 0885-5765

VL - 102

SP - 128

EP - 135

JO - Physiological & Molecular Plant Pathology

JF - Physiological & Molecular Plant Pathology

ER -

Identification and pathogenicity of Fusarium species associated with sesame (Sesamum indicum L.) seeds from the Punjab, Pakistan

Abstract

Bibliographical note

Keywords

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