Identification by polymerase chain reaction (PCR) of Marshallagia marshalli and Ostertagia gruehneri from Svalbard reindeer

J F Dallas, R J Irvine, O Halvorsen, S D Albon

    Research output: Contribution to journalArticle

    20 Citations (Scopus)

    Abstract

    A polymerase chain reaction (PCR) assay to identify two common abomasal nematodes Marshallagia marshalli and Ostertagia gruehneri of Svalbard reindeer was developed. Species-specific PCR primers were designed from internal transcribed spacer (ITS)-2 sequences of rDNA and validated using morphologically identified adult male and female nematodes. Using the species-specific primers, a 110 bp fragment was amplified from M. marshalli and its minor morph Marshallagia occidentalis and a 149 bp fragment was amplified from Ostertagia gruehneri and its minor morph Ostertagia arctica. No PCR products were amplified from the third rare species, Teladorsagia circumcincta, or DNA from the reindeer host. The assay provides a useful tool to estimate species composition for both sexes in this nematode community. (C) 2000 Australian Society for Parasitology Inc. Published by Elsevier Science Ltd. All rights reserved.

    Original languageEnglish
    Pages (from-to)863-866
    Number of pages4
    JournalInternational Journal for Parasitology
    Volume30
    Publication statusPublished - 2000

    Keywords

    • Marshallagia marshalli
    • Ostertagia gruehneri
    • Teladorsagia circumcincta
    • nematoda
    • species identification
    • ribosomal DNA
    • internal transcribed spacer-2
    • polymerase chain reaction
    • GASTROINTESTINAL NEMATODES
    • RIBOSOMAL DNA
    • WILD RUMINANTS
    • SYSTEMATICS
    • ITS-2
    • RDNA
    • EGGS

    Cite this

    Identification by polymerase chain reaction (PCR) of Marshallagia marshalli and Ostertagia gruehneri from Svalbard reindeer. / Dallas, J F ; Irvine, R J ; Halvorsen, O ; Albon, S D .

    In: International Journal for Parasitology, Vol. 30, 2000, p. 863-866.

    Research output: Contribution to journalArticle

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    abstract = "A polymerase chain reaction (PCR) assay to identify two common abomasal nematodes Marshallagia marshalli and Ostertagia gruehneri of Svalbard reindeer was developed. Species-specific PCR primers were designed from internal transcribed spacer (ITS)-2 sequences of rDNA and validated using morphologically identified adult male and female nematodes. Using the species-specific primers, a 110 bp fragment was amplified from M. marshalli and its minor morph Marshallagia occidentalis and a 149 bp fragment was amplified from Ostertagia gruehneri and its minor morph Ostertagia arctica. No PCR products were amplified from the third rare species, Teladorsagia circumcincta, or DNA from the reindeer host. The assay provides a useful tool to estimate species composition for both sexes in this nematode community. (C) 2000 Australian Society for Parasitology Inc. Published by Elsevier Science Ltd. All rights reserved.",
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    AU - Dallas, J F

    AU - Irvine, R J

    AU - Halvorsen, O

    AU - Albon, S D

    PY - 2000

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    AB - A polymerase chain reaction (PCR) assay to identify two common abomasal nematodes Marshallagia marshalli and Ostertagia gruehneri of Svalbard reindeer was developed. Species-specific PCR primers were designed from internal transcribed spacer (ITS)-2 sequences of rDNA and validated using morphologically identified adult male and female nematodes. Using the species-specific primers, a 110 bp fragment was amplified from M. marshalli and its minor morph Marshallagia occidentalis and a 149 bp fragment was amplified from Ostertagia gruehneri and its minor morph Ostertagia arctica. No PCR products were amplified from the third rare species, Teladorsagia circumcincta, or DNA from the reindeer host. The assay provides a useful tool to estimate species composition for both sexes in this nematode community. (C) 2000 Australian Society for Parasitology Inc. Published by Elsevier Science Ltd. All rights reserved.

    KW - Marshallagia marshalli

    KW - Ostertagia gruehneri

    KW - Teladorsagia circumcincta

    KW - nematoda

    KW - species identification

    KW - ribosomal DNA

    KW - internal transcribed spacer-2

    KW - polymerase chain reaction

    KW - GASTROINTESTINAL NEMATODES

    KW - RIBOSOMAL DNA

    KW - WILD RUMINANTS

    KW - SYSTEMATICS

    KW - ITS-2

    KW - RDNA

    KW - EGGS

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    JO - International Journal for Parasitology

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