Abstract
A polymerase chain reaction (PCR) assay to identify two common abomasal nematodes Marshallagia marshalli and Ostertagia gruehneri of Svalbard reindeer was developed. Species-specific PCR primers were designed from internal transcribed spacer (ITS)-2 sequences of rDNA and validated using morphologically identified adult male and female nematodes. Using the species-specific primers, a 110 bp fragment was amplified from M. marshalli and its minor morph Marshallagia occidentalis and a 149 bp fragment was amplified from Ostertagia gruehneri and its minor morph Ostertagia arctica. No PCR products were amplified from the third rare species, Teladorsagia circumcincta, or DNA from the reindeer host. The assay provides a useful tool to estimate species composition for both sexes in this nematode community. (C) 2000 Australian Society for Parasitology Inc. Published by Elsevier Science Ltd. All rights reserved.
Original language | English |
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Pages (from-to) | 863-866 |
Number of pages | 4 |
Journal | International Journal for Parasitology |
Volume | 30 |
Publication status | Published - 2000 |
Keywords
- Marshallagia marshalli
- Ostertagia gruehneri
- Teladorsagia circumcincta
- nematoda
- species identification
- ribosomal DNA
- internal transcribed spacer-2
- polymerase chain reaction
- GASTROINTESTINAL NEMATODES
- RIBOSOMAL DNA
- WILD RUMINANTS
- SYSTEMATICS
- ITS-2
- RDNA
- EGGS