Identification, expression analysis, genomic organization and cellular location of a novel protein with a RhoGEF domain

Sze-Wah Tse, Jennifer A Broderick, Mei-Ling Wei, Min-Hua Luo, Deborah Smith, Peter John Andrew McCaffery, Stefan Stamm, Athena Andreadis

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

In this study we describe the identification and characterization of a novel cytosolic protein of the guanine exchange factor (GEF) family. The human cDNA corresponds to predicted human protein FLJ00128/FLJ10357 located on chromosome 14q11.2. The deduced protein sequence contains in its C-terminus a RhoGEF domain followed by a pleckstrin domain. Its N-terminus, central region and RhoGEF/pleckstrin domain are homologous to the recently identified zebrafish Quattro protein, which is involved in morphogenetic movements mediated by the actin cytoskeleton. Based on the homology of our protein's RhoGEF domain to the RhoGEF domains of Trio, Duo and Duet and its homology with Quattro, we named it Solo. The Solo mRNA is ubiquitously expressed but enriched in brain, its expression peaks perinatally and it undergoes extensive alternative splicing. In both myoblasts and neuroblastoma cells, the Solo protein is concentrated around the nucleus.
Original languageEnglish
Pages (from-to)63-72
Number of pages10
JournalGene
Volume359
DOIs
Publication statusPublished - 10 Oct 2005

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Keywords

  • amino acid sequence
  • animals
  • blood proteins
  • blotting, northern
  • blotting, western
  • brain
  • carrier proteins
  • cell line
  • cell line, tumor
  • molecular cloning
  • cytoplasm
  • complementary DNA
  • exons
  • gene expression profiling
  • guanine nucleotide exchange factors
  • humans
  • Immunohistochemistry
  • Immunoprecipitation
  • Introns
  • male
  • mice
  • molecular sequence data
  • phosphoproteins
  • protein binding
  • messenger RNA
  • rats
  • reverse transcriptase polymerase chain reaction
  • Saccharomyces cerevisiae
  • sequence alignment
  • DNA sequence analysis
  • sequence homology, amino acid
  • two-hybrid system techniques
  • tau proteins

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